Prostat Kanserinde Selenyumun Proteomik Temelli Bir Yaklaşımla Farklı Etkilerinin Belirlenmesi

Öz

Serum prostat spesifik antijeni (PSA) prostat kanserli hastaların tanımlanmasında kliniğe çok önemli katkı sağlamıştır. Bununla birlikte PSA’nın bazı limitasyonları bulunmaktadır. Bu durum erken tanı ve tedavide yeni biomarkerların tanımlanması ihtiyacını doğurmuştur. Klinik çalışmalara göre, selenyumun ileri ve agresif prostat kanserinde potansiyel önleyici etkisinin olduğu gösterilmiştir. Ancak bu etki mekanizmasının daha çok düşük selenyuma sahip erkeklerde etkili olduğu gösterilmiştir. Bu çalışmanın amacı, yeni tümör markerlarının araştırılması ve selenyumla indüklenen değişikliklerin proteomiks teknolojisiyle saptanmasıdır. Selenyumla muamale edilmiş BPH-1 ve LNCaP hücrelerinin bulunduğu besiyerindeki proteinler iki boyutlu elektroforez yapılmış ve MALDI-TOF-MS ile tanımlanmıştır. MALDI-TOF-MS kullanılarak ve veri tabanı araştırılması yapılarak besiyerinde eksprese olan 9 farklı protein tanımlanmıştır. Proteomikse dayalı bir yöntemle yeni tümör markerlarının araştırılması için uygun ve etkin bir yol olarak prostat kanseri ve benign prostatik hipertrofi hücrelerinin ayırımında 9 aday protein bir kemopreventif ajan olan selenyum kullanımına yanıt vermesine bağlı olarak tanımlanmıştır.

 

 

Anahtar Kelimeler

• Prostat kanseri,proteomik,selenyum

Prostat Kanserinde Selenyumun Proteomik Temelli Bir Yaklaşımla Farklı Etkilerinin Belirlenmesi

Öz

Although the induction of serum prostate specific antigen (PSA) measurements in clinical practice has been considered revolutionary in the treatment of prostate cancer in patients, accepted constraints still remain in the usage of PSA. Hence, it is crucial to identify other prostate cancer biomarkers that could contribute to early detection and prognosis. According to the clinical trials, selenium shows its potential inhibitory effects in advanced-stage or aggressive prostate cancer. But this chemopreventive effect shows inhibitory action only in men with low selenium levels. The aim of this study was to search new tumor markers and selenium induced changes utilizing proteomics technology. After profiling of the proteins found in conditioned medium (CM) of selenium treated BPH-1 and LNCaP cells via two-dimensional electrophoresis, identification was performed by matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF-MS). Nine differentially expressed proteins were identified in the CM of BPH-1 and LNCaP cells using MALDI-TOF-MS and database search. Conclusion: We procured possible and efficient way to search new tumor marker candidates by a proteomics-based analysis and identifed 9 potential candidate proteins in CM of selenium treated BPH-1 and LNCaP cells to differentiate prostate cancer response from benign prostatic hypertrophy response.

 

 

Anahtar Kelimeler

• Prostate cancer,proteomics,selenium

Kaynakça

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