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Öz

treatment arranged factorially with 2 feed form (mash and pellet) and 2 cynarin doses (none, 0.25g/kg). These groups were; mash form (D1), pellet form (D2), mash form+Cynarin (0.25g/kg feed) (D3), and pellet form+Cynarin (D4). The duration o f the trials was 42 days. I n this study, tibia weight, ash rate, tibia Ca and P concentration and some blood parameters were evaluated. The average tibia weight o f the groups differed depending on the form o f the feed (P<0.05), however it was observed that tibia ash ratio and bone mineral content have not changed depending on the diets. On day 21 it was determined that interaction between feed form and cynarin effected on serum urea values (P<0.05). Serum triglyceride value decreased (P<0.001) depending on the Cynarin supplementation in the diets and the form o f the feed had an important role in this effect (P<0.03). On day 42, there were no effects o f applications on selected serum parameters. Cynarin supplementation decreased serum phosphor (iP) (P<0.0001) and calcium (Ca) (P<0.006) values on day 21 and the form o f the feed was also found important i n this changes (P<0.002). During the same period, serum Alkaline phosphatase values also differed depending on the form o f the diet (P<0.036). On day 42, negative effect o f cynarin supplementation on iP concentration continued (P<0.0001). I n conclusion, it was determined that cynarin supplementation decreased serum triglyceride concentration, however, the form o f feed was more effective on the bone structure

Anahtar Kelimeler

• Broiler, cynarin, feed form, tibia, some serum indices

Farklı Yem Formundaki Broyler Diyetlerine Bir Bitki Ekstrakt İçeriği Olan Cynarin İlavesinin Kemik Yapı ve Bazı Kan Parametreleri Üzerine Etkisi

Öz

Bu çalışmada, diyetlerine bir bitki ekstrakt içeriği olan Cynarin ilavesinin broylerlerde kemik yapı ile bazı kan parametreleri üzerine etkileri ve bu etkide yem formunun rolü incelenmiştir. Araştırmada 480 adet günlük broyler civciv (Ross 308) kullanılmıştır. Her grupta 120 hayvan olacak şekilde 4 eşit gruba ayrılan hayvanlarda çalışma 6 tekrarlı yapılmıştır. Hayvanlara verilen diyetler başlangıç, civciv ve piliç dönemleri için ayrı ayrı hazırlanmıştır. Deneme grupları yem formuna göre iki ana gruba ve her ana grup kendi arasında iki alt gruba ayrılmıştır. Bu gruplar; Toz formu (D1), Pelet Formu (D2), Toz formu+Cynarin (0,25 g/kg yem) (D3) ve Pelet Formu+Cynarin (D4)'dir. Deneme 42 gün sürmüştür. Çalışmada, tibia ağırlığı, kül oranı ile kemik kalsiyum (Ca) ve fosfor (P) içerikleri ile birlikte bazı kan parametreleri değerlendirilmiştir. Deneme grupların ortalama tibia ağırlıklarının yem formuna göre değiştiği saptanmış (P < 0,005) ancak tibia kül oranları ve kemik mineral içerikleri üzerine ise uygulamaların bir etkisinin olmadığı bulunmuştur. Çalışmanın 21. gününde serum üre değerleri üzerine yem formu ve Cynarin arasındaki etkileşimin etkili olduğu saptanmıştır (P < 0,05). Serum trigliserit oranında ise diyete Cynarin ilave edilmesine bağlı olarak bir azalış göstermiş (P < 0,001), diyet formunun da bu azalışta önemli olduğu saptanmıştır (P < 0,03). Araştırmanın 42. gününde ise uygulamaların seçilen kan parametreleri üzerine bir etkisi belirlenememiştir. Denemenin 21. gününde diyete Cynarin ilavesi ile serum fosfor (iP) (P < 0,0001) ve Ca değerlerinin düştüğü (P < 0,006) ve bu düşüşte yemin toz formda olmasının da önemli olduğu saptanmıştır (P < 0,002). Aynı dönemde serum Alkalin fosfataz düzeyi de diyet formuna bağlı olarak değişim göstermiştir (P < 0,036). Çalışmanın 42. gününde Cynarin ilavesinin iP konsantrasyonu üzerine olan olumsuz etkisi devam etmiştir (P < 0,0001). Sonuç olarak, yapılan bu çalışmada yeme Cynarin ilavesinin serum trigliserit oranını düşürücü etkisi saptanmış ve kemik yapısı üzerine yem formunun daha etkin olduğu belirlenmiştir.

Anahtar Kelimeler

• Broyler, cynarin, yem formu, tibia, bazı kan parametreleri

Kaynakça

1. Abdo, Z . M . A . , Radvan, N . L . , Selim, N.A., 2007. The effect o f artichocke leaves meal on utilization o f dietary energy for broiler chicks. International Journal o f Poultry Science 6, 973-982.
2. Ahmad, T . , Rasool, M . , Sarwar, M . , H a q , A . , Z i a ul Hasan., 2000. Effect of microbial phytase produced from a fungus Aspergillus niger on bioavailability o f phosphorus and calcium i n broiler chickens. Animal Feed Science and Technology 83, 103-114.
3. Amerah, A . M . , Ravindran, V . , Lentle, R . G . Thomas, D . G . , 2007. Feed particle size: Implications on the digestion and performance o f poultry. World's Poultry Science Journal 63, 439-4
4. A O A C , 1994. Official Methods o f Analysis. Association o f Official Analytical Chemists. Washington DC.
5. Brenes, A . , Viveros, A . , A r i j a , I . , Centeno, C . , Pizarro, M . , Bravo, C . , 2003. The effect o f citric acid and microbial phytase on mineral utilization i n broiler chicks. A n i m a l Feed Science and Technology 110, 201-219.
6. Brickett, K . E . , Dahiya, J . P . , Classen, H . L . , Annet, C . B . , Gomis, S., 2007. The impact o f nutrient density, feed form, and photoperiod on walking ability and skeletal quality o f broiler chickens. Poultry Science 86, 2117-2125.
7. Engberg, R . M . , Hedemann, M . S . , Jensen, B . B . , 200 The influence o f grinding and pelleting o f feed on the microbialcomposition and activity i n the digestive tract o f broiler chickens. British Poultry Science 43, 569-579. Gebhardt, R . , 1997. Antioxidative and protective properties o f extract from leaves o f the artichoke (Cynara scolymus L . ) against hydroperoxide-induced oxidative stress i n cultured rat hepatocytes. Toxicology and Applied Pharmacology 144, 286-297.
8. Jin, S.H., Corless, A . , Sell, J . L . , 1998. Digestive system development i n post-hatch poultry. World's Poultry Science Journal 54, 335-340.

9. Katongole, J . B . D . , March, B . E . , 1980. Fat utilization i n relation to intestinal faty acid binding protein and bile salts i n chicks o f different ages and different genetic sources. Poultry Science 59, 819-8
10. Kilburn, J . , Edwards, H . M . , 2001. The response of broilers to the feeding o f mash or pelleted diets containing maize o f varying particle size. British Poultry Science 42, 484-492.
11. Krogdahl, A . , 1985. Digestion and absorption o f lipid i n poultry. Journal of Nutrition 115, 675-685.
12. Kraft, K . , 1997. Artichoke leaf extract-recent findings reflecting effects on l i p i d metabolism, liver, and gastrointestinal tracts. Phytomedicine 4, 369¬ 3
13. Llorach, R . , Espin, J . C . , Tomas-Barberan, F . A . , Ferreres, F . , 2002. Artichoke (Cynara scolymus L . ) by products as a potential source o f health-promoting antioxidant phenolics. Journal of Agricultural and Food Chemistry 50, 3458-3464.
14. Moglia, A . , Lanteri, S., Comino, C . , Acquadro, A . , De Vos, R . , Beekwilder, J . , 2008. Stressinduced biosynthesis o f dicaffeoylquinic acids i n globe artichoke. Journal of Agricultural and Food Chemistry 56, 8641-8649.
15. Moss, D . W . , 1982. Alkaline phosphatase isoenzymes.
16. Clinical Chemistry 28, 2007-2016.
17. Nir, I . , Twina, Y . , Grossman, E . , Nitsan, Z . , 1994.
18. Quantitative effect of peleting on performance gastrointestinal tract and behavior o f meat type chickens. British Poultry Science 35, 589-602. National Research Council ( N R C ) , 1994. Nutrient
19. Requirements o f Poultry. 9 t h rev. Ed. National Academy Press, Washington DC. Qian, H . , Kornegay, E . T . , Denbow, D . M . , 1996.
20. Phosphorus equivalence o f microbial phytase i n turkey diets as influenced by ca1cium to phosphorus rations and phosporus levels. Poultry Science 75, 69-81. Radwan, N . L . , Abdo, Z . M . A . , Hassan, R . A . , 2007.
21. Effect o f feeding artichocke leaves meal on productive anad reproductive performance o f Mandarah Hens. International Journal o f Poultry Science 6, 826-834. Ravindran, V . , Cabahug, S., Ravindran, G . , Selle, P . H . , Bryden, V . L . , 2000. Respons of broiler chickens to microbial phytase supplementation as influenced by dietary phytic acid and non phytate phosphorus levels. I I . Effects on apparent metabolisable energy, nutrient Farklı Yem Formundaki Broyler Diyetlerine Bir Bitki Ekstrakt içeriği Olan Cynarin ilavesinin Kemik Yapı ve Bazı Kan Parametreleri Üzerine Etkisi 19 digestibility and nutrient retention. British Poultry Science 4 1 , 193-200.
22. Saenz, R . T . , Garcia, G . D . , dela Puerta, V . R . , 2002. Choleretic activity and biliary elimination o f lipids and bile acids induced by an artichoke leaf extract i n rats. Phytomedicine 9, 687-693.
23. Scott, T . A . , 2002. Evaluation o f lighting programs, diet density, and short-therm use o f mash as compared to crumbled starter to reduce incidence of sudden death syndrome i n broiler chicks to 35 days o f age. Canadian Journal o f Animal Science 82, 375-383.
24. Schütz, K . , Kammerer, D., Carle, R . , Schieber, A . , 200 Identification and quantification o f caffeoylquinic acids and flavonoids from artichoke (Cynara scolymus) heads, juice and pomace by H P L C - D A D - E S I / M S . Journal o f Agricultural and Food Chemistry 52, 4090¬ 40
25. Skinner-Noble, D . O . , McKinkey, L . J . , Teeter, R . G . , 200 Predicting effective caloric value o f nonnutritive factors: I I I . Feed form affects broiler performance by modifying behavior patterns. Poultry Science 84, 403-411. Snedecor, G . W . , Cochran, W . G . , 1980. Statistical Methods, 7 t h ed., The Iowa State Univ. Press. Ames., Iowa.
26. Speroni, E . , Cervellati, R . , Govoni, P., Guizzardi, S., Renzulli, C . , G u e r r a , M . C . , 2003. Efficacy o f different Cynara scolymus preparations on liver complaints. Journal o f Ethnopharmacology 86, 203-2
27. SPSS, 1999. SPSS for Windows. Base System User's
28. Guide, Release 10.1, SPSS Inc. Chicago I L , USA, 1999.
29. Stoev, S.D., Djuvinov, D., Mirtchevac, T . , Pavlov, D., Mantlee, P., 2002. Studies on some feed additives giving partial protection against ochratoxin A toxicity i n chicks. Toxicology Letter 135, 33-50.
30. Svihus, B . , Klovstad, K . H . , Perez, V . , Zimonja, O., Sahlstrom, S., Schuller, R . B . , 2004. Physical and nutritional effects o f pelleting o f broiler chicken diets made from wheat ground to different coarsenesses by the use o f roller m i l l and hammermill. A n i m a l Feed Science and Technology 117, 281-293.
31. Uni, Z . , Noy, Y . , Sklan, D . , 1995. Posthach changes i n morphology and function o f the small intestine i n heavy and light strain chicks. Poultry Science 74, 1622-1629.
32. Viveros, A . , Brenes, A . , A r i j a , I . , Centeno, C . , 2002.
33. Effects o f phytase supplementation on mineral utilization and serum enzyme activities i n broiler chicks fed different levels o f phosphorus. Poultry Science 8 1 , 1172-1183.
34. Wisser, L . A . , Heinrichs, B.S., Leach, R . M . , 1990.
35. Effect aluminum on performance and mineral metabolism i n young chicks and laying hens. Journal o f Nutrition 120, 493-498. Z h u , X . , Zhang, H . , L o , R . , 2004. Phenolic compounds from leaf extract o f artichoke
36. (Cynara scolymus) and their antimacrobial activities. Journal o f Agricultural and Food Chemistry 52, 7272-7278.