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Öz

Insulin-like growth factor-I (IGF-I) is one of the most important metabolic factors that plays a critical role in cow fertility. Recently, point mutation of IGF-I gene at nucleotide position 512 (C/T transition) within the 5′-untranslated region (5'-UTR) has attracted the most attention. It has previously been demonstrated that there was an important relationship between this type of mutation and IGF-I concentration in dairy cows serum. Hence, we investigate the relationship between polymorphism within 512 of the 5'-UTR of IGF-I gene and some reproductive parameters in Iranian Holstein dairy cows. Blood samples were collected from 60 multiparous cows with a history of desirable fertility (SPC<2; n=25) and undesirable fertility (SPC 2; n=35) based on the previous lactation numbers. The results showed that higher percentage (65%) of cows with CC genotype had desirable fertility (SPC<2); while a lower percentage of cows with TC (33.3%) and TT genotypes (14.3%) had good fertility (P<0.05). Therefore, the CC genotype was associated (P<0.05) with desirable fertility. In addition, the IGF-I mutation had a significant effect on service per conception and days open in which cows with TT genotype and TC genotype had greater average of services per conception and days open compared to cows with the CC genotypes (P<0.05). It can be concluded that the C/T mutation within this region of IGF-I gene may influence the reproductive parameters in Holstein dairy cows. These data support the hypothesis that the mutation in this site might be considered as a genetic marker for reproductive performance in Holstein dairy cows.

Anahtar Kelimeler

• Insulin-like growth factor-I (IGF-I), polymorphism, fertility, dairy cow

Assessment of Single Nucleotide Polymorphism in the 5'-Flanking Region of Insulin-Like Growth Factor-I (IGF-I) Gene as a Potential Genetic Marker for Fertility in Holstein Dairy Cows

Öz

ABSTRACT

Insulin-like growth factor-I (IGF-I) is one of the most important metabolic factors that plays a critical role in cow fertility. Recently, point mutation of IGF-I gene at nucleotide position 512 (C/T transition) within the 5′-untranslated region (5'-UTR) has attracted the most attention. It has previously been demonstrated that there was an important relationship between this type of mutation and IGF-I concentration in dairy cows serum. Hence, we investigate the relationship between polymorphism within 512 of the 5'-UTR of IGF-I gene and some reproductive parameters in Iranian Holstein dairy cows. Blood samples were collected from 60 multiparous cows with a history of desirable fertility (SPC<2; n=25) and undesirable fertility (SPC³2; n=35) based on the previous lactation numbers. The results showed that higher percentage (65%) of cows with CC genotype had desirable fertility (SPC<2); while a lower percentage of cows with TC (33.3%) and TT genotypes (14.3%) had good fertility (P<0.05). Therefore, the CC genotype was associated (P<0.05) with desirable fertility. In addition, the IGF-I mutation had a significant effect on service per conception and days open in which cows with TT genotype and TC genotype had greater average of services per conception and days open compared to cows with the CC genotypes (P<0.05). It can be concluded that the C/T mutation within this region of IGF-I gene may influence the reproductive parameters in Holstein dairy cows. These data support the hypothesis that the mutation in this site might be considered as a genetic marker for reproductive performance in Holstein dairy cows.

Key Words: Insulin-like growth factor-I (IGF-I), polymorphism, fertility, dairy cow

 

ÖZET

SİYAH ALACA SIĞIRLARINDA İNSÜLİN BENZERİ BÜYÜME FAKTÖRÜ-I (IGF-I) GENİNİN
5'- SINIRDAŞ BÖLGESİNDEKİ TEK NÜKLEOTİD POLİMORFİZMİNİN FERTİLİTE İÇİN POTANSİYEL GENETİK MARKIR OLARAK DEĞERLENDİRİLMESİ

İnsulin benzeri büyüme faktörü-I (IGF-I) inek fertilitesinde kritik bir rol oynayan en önemli metabolik faktörlerden birisidir. Son yıllarda IGF-I geninin 5'- kodlanmayan bölgesinde (5'-UTR) 512. nükleotid pozisyonundaki (C/T dönüşümü) nokta mutasyonu oldukça ilgi çekmiştir. Bu tip mutasyon ile süt ineği serumundaki IGF-I konsantrasyonu arasında önemli bir ilişki bulunduğu bildirilmiştir. Bu nedenle İran Siyah Alaca sütçü ineğine ait bazı reprodüktif parametreler ile IGF-I geninin 5'-UTR bölgesinde 512. nükleotidindeki polimorfizm arasındaki ilişkiyi inceledik. Kan örnekleri, daha önceki laktasyonlarında istenilen düzeyde fertilite gösteren (SPC<2; n = 25) ve göstermeyen (SPC³2; n=35), daha önce buzağılamış toplam 60 adet inekten toplanmıştır. İneklerin yüksek bir yüzdesi (%65) CC genotipine ve istenilen fertilite düzeyine sahip oldukları (SPC<2); ineklerin daha düşük bir yüzdesinin de (%33) TC ve TT (%14,3) genotiplerine ve iyi fertiliteye sahip oldukları (P<0,05) tespit edilmiştir. Bu nedenle CC genotipi istenilen fertilite düzeyi ile bağlantılıdır (P<0,05). Ayrıca, IGF-I mutasyonunun gebelik başına tohumlama sayısı ve servis periyodu süresi üzerine önemli derecede etkili olduğu bulunmuştur. TT ve TC genotipine sahip ineklerin CC genotipine sahip olanlara göre daha yüksek gebelik başına tohumlama sayısı ve servis periyodu süresi ortalamasına sahip oldukları tespit edilmiştir (P < 0,05). Bu çalışmada sonuç olarak IGF-I geninin bu bölgesinde meydana gelen C/T mutasyonu Siyah Alaca ineklerde reprodüktif parametreleri etkileyebileceği sonucuna ulaşılmıştır. Elde edilen sonuçlar, bu bölgedeki mutasyonun Siyah Alaca ineklerde genetik işaretleyici olarak kullanılabileceği hipotezini desteklemektedir.

Key Words: İnsülin benzeri büyüme faktörü-I (IGF-I), polimorfizm, fertilite, süt ineği

 

 

Anahtar Kelimeler

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Kaynakça

1. Becker, K.L., 2001. Principles and Practice of Endocrinology and Metabolism. 3 rd ed. Lippincott Williams and Wilkins Company pp. 1588-1592.
2. Bonakdar, E., Rahmani, H.R., Edriss, M.A., Tabatabaei, B.E.S., 20 IGF-I gene polymorphism, but not its blood concentration, is associated with milk fat and protein in Holstein dairy cows. Genetic and Molecular Research 9, 1726-1734.
3. Cazzola, M., Skoda, R.C., 2000. Translational pathophysiology: a novel molecular mechanism of human disease. Blood 95, 3280-3288.
4. Chase, C.C., Kirby, C.J., Hammond, A.C., Olson, T.A., Lucy, M.C., 1998. Patterns of ovarian growth and development in cattle with a growth hormone receptor deficiency. Journal of Animal Science 76, 212-219.
5. Daftary, S.S., Gore, A.C., 2005. IGF-I in the brain as a regulator of reproductive neuroendocrine function. Experimental Biology Medicine 230, 292-30
6. Ge, W., Davis, M.E., Hines, H.C., Irvin, K.M., Simmen, R.C.M., 2001. Association of a genetic marker with blood serum insulin-like growth factor-I concentration and growth traits in Angus cattle. Journal of Animal Science 79, 1757-1762.
7. Hines, H.C., Ge, W., Zhao, Q., Davis, M.E., 1998. Association of genetic markers in growth hormone and insulin-like growth factor - I loci with lactation traits in Holsteins. Animal Genetic 29 (Suppl 1), 69-74.
8. Kadivar, A., Ahmadi, M.R., Gheisari, H.R., Nazifi, S., 2012. Assessment of IGF-I as a factor influencing postpartum reproductive performance and ovarian condition in dairy cattle. Comparative Clinical Pathology 21, 589-596.

9. Kawashima, C., Fukihara, S., Maeda, M., Kaneko, E., Montoya, C.A., Matsui, M., Shimizu, T., Matsunaga, N., Kida, K., Miyake, Y.I., Schams, D., Miyamoto, A., 2007. Relationship between metabolic hormones and ovulation of dominant follicle during the first follicular wave post-partum in high-producing dairy cows. Reproduction 133, 155-163.
10. Kirkpatrick, B.W., 1992. Identification of a coserved micro satellite site in the porcine and bovine insulin - like growth factor-1 gene 5 flank. Animal Genetic 23, 543-548.
11. Kirkpatrick, B.W., 1993. Dialleic single-strand conformation polymorphism in the bovine insulin-like growth factor-1 third intron. Animal Genetic 24, 144-148.
12. Kozak, M., 1991. An analysis of vertebrate mRNA sequences: intimations of translational control. Journal of Cell Biology 115, 887-903.
13. Lien, S., Karlsen, A., Klemetsdal, G., Vage, D.I., Olsaker, I., Klungland, H., Aasland, M., Heringstad, B., Ruane, J., Gomez-Raya, L., 2000. A primary screen of the bovine genome for quantitative trait loci affecting twinning rate. Mammalian Genome 11, 877-882.
14. Lucy, M.C., 2000. Regulation of ovarian follicular growth by somatotropin and insulin-like growth factors in cattle. Journal of Dairy Science 83, 1635-1647. Maj, A., Snochowski, M., Siadkowska, E., Rowinska, B., Lisowski, P., Robaka-Hyzorek, D., Oprzadek, J., Grochowska, R., Kochman, K., Zwierzchowski, L., 2008. Polymorphism in gene of growth hormon receptor (GHR) and insulin-like growth factor-1(IGF1) and its association with both the IGF1expression in liver and its level in blood in Polish Holstein – Friesian cattle. Neuroendocrinology Letters 29(6), 981-989.
15. Mehmannavaz, Y., Amirinia, C., Bonyadi, M., Torshizi, R.V., 2010. Association of IGF-I gene polymorphism with milk production traits and paternal genetic trends in Iranian Holstein bulls. African Journal of Microbiology and Research 4(1), 110-114.
16. Monget, P., Monniaux, D., 1995. Growth factors and control of folliculogenesis. Journal of Reproduction and Fertility (Suppl.) 49, 321-333.
17. Moyes, T.E., Stockdale, C.R., Humphrys, S., Macmilan, K.L., 2004. Concentrations of IGF1 in plasma of Holstein cows are negatively associated with reproductive performance and milk yield and positively associated with milk composition. Abstracts for the 34 th Annual Conferenes of the Society for Reproductive Biology pp. 21.
18. Reynolds, P.R., 2002. In sickness and in health: the importance of translational regulation. Archives of Disease in Childhood 86, 322-324.
19. Roberts, A.J., Klindt, J., Jenkins, T.G., 2005. Effects of varying energy intake and sire breed on duration of postpartum anestrus, insulin like growth factor-1, and growth hormone in mature crossbred cows. Journal of Animal Science 83, 1705-1714.
20. Ruprechter, G., Carriquiry, M., Ramos, J.M., Pereira, I., Ana, M., 2011. Metabolic and endocrine profiles and reproductive parameters in dairy cows under grazing conditions: effect of polymorphisms in somatotropic axis genes. ACTA Veterinary Scandinavia 53, 35-44.
21. Siadkowska, E., Zwierzchowski, L., Oprzadek, L., Strzalkowska, N., Bagnicka, E., Krzyzewski, J., 2006. Effect of polymorphism in IGF1 gene on production traits in Polish Holstein-Friesian cattle. Animal Science Papers and Reports 24(3), 225-237.
22. Smith, L., 2008. Post-transcriptional regulation of gene expression by alternative 5’-untranslated regions in carcinogenesis. Biochemical Society Transactions 36, 708-711.
23. Spicer, L.J., Tinker, E.D., Wettemann, R.P., Buchanan, D.S., 1993. Relationship between genotype of sire and dam, and plasma insulinlike growth factor-1 concentrations in crossbred cows post partum. Livestock Production Science 33, 355-360.
24. Stefanello, J.R., Barreta, M.E., Porciuncula, P.M., Arruda, J.N., Oliveira, J.F., Oliveira, M.A., Goncalves, P.B., 2006. Effect of angiotensin II with follicle cells and insulin-like growth factor-I or insulin on bovine oocyte maturation and embryo development. Theriogenology 66(9), 2068-2076.
25. Stirling, T.E., Stockdale, C.R., Macmillan, K.L., 200 Heritability of plasma concentrations of IGF1 and its correlation with reproductive performance in Holstein cows in victorian herds. Proc. Proceedings of the 18th Conference Barossa Valley, South Australia, Proceeding Association Advancement Animal Breeding Genetic 18, 648-651. Swali, A., Wathes, D.C., 2006. Influence of the dam and sire on size at birth and subsequent growth, milk production and fertility in dairy heifers. Theriogenology 66, 1173-1184.
26. Tamadon, A., Kafi, M., Saeb, M., Mirzaei, A., Saeb, S., 2011. Relationships between insulin-like growth factor-I, milk yield, body condition score, and postpartum luteal activity in highproducing dairy cows. Tropical Animal Health and Production 43, 29-34.
27. Taylor, V.J., Cheng, Z., Pushpakumara, P.G.A., Beever, D.E., Wathes, D.C., 200 Relationships between the plasma concentrations of insulin-like growth factor-I in dairy cows and their fertility and milk yield. Veterinary Record 155, 583-588. Velazquez, M.A., Newman, M., Christie, M.F., Cripps, P.J., Crowe, M.A., Smith, R.F., Dobson, H., 2005. The usefulness of a single measurement of insulin-like growth factor-1 as a predictor of embryo yield and pregnancy rates in a bovine MOET program. Theriogenology 64, 1977-1994.
28. Velazquez, M.A., Spicer, L.J., Wathes, D.C., 2008. The role of endocrine insulin-like growth factor-I (IGF-I) in female bovine reproduction. Domestic Animal Endocrinology 35, 325-342.
29. Watson, A.J., Westhusin, M.E., Winger, Q.A., 1999. IGF paracrine and autocrine interactions between conceptus and oviduct. Journal of Reproduction and Fertility 54(Suppl.), 303-315. Zulu, V.C., Sawamukai, Y., Nakada, K., Kida, K., Moriyoshi, M., 2002. Relationship among insulin-like growth factor-I, blood metabolites and postpartum ovarian function in dairy cows. Journal of Veterinary Medical Science 64, 8798