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Öz

Dorema aucheri is a plant from the Apiaceae family, which has several compounds such as flavonoids and kumarines. Flavonoids have antitumor, anticancer and estrogenic properties. Antiandrogenic properties of kumarines are also known. The aim of this research is to examine the Dorema aucheri hydroalcoholic extract effects on SOD and GPX activities and serum concentration of vitamins E and C. Sixty adult male Wistar rats weighing about 200 to 220 g were divided into four groups of fifteen. The control group did not receive any drug. The other three experimental groups including low (100 mg/kg BW), medium (200 mg/kg BW) and maximum (400 mg/kg BW) received Dorema aucheri hydroalcoholic extract daily for 28 days orally. After 14 and 28 days all animals in the different groups were weighed and their blood collected, and SOD and GPX activities, and vitamin E and C concentrations were measured by validated standard methods. The results showed that in the short term, SOD and GPX activities, as well as vitamin C concentration increase depending on the dose, but in the long term these parameters decrease proportional to the increase of the dosage. Vitamin E concentration had no significant changes during our study.

Anahtar Kelimeler

• Dorema aucheri hydroalcoholic extract, antioxidant enzymes, antioxidant vitamins

The Effects of Dorema aucheri Hydroalcoholic Extract on Blood Levels of Antioxidant Enzymes (SOD and GPX) and Vitamins (E and C) in vivo

Öz

ABSTRACT

Dorema aucheri is a plant from the Apiaceae family, which has several compounds such as flavonoids and kumarines. Flavonoids have antitumor, anticancer and estrogenic properties. Antiandrogenic properties of kumarines are also known. The aim of this research is to examine the Dorema aucheri hydroalcoholic extract effects on SOD and GPX activities and serum concentration of vitamins E and C. Sixty adult male Wistar rats weighing about 200 to 220 g were divided into four groups of fifteen. The control group did not receive any drug. The other three experimental groups including low (100 mg/kg BW), medium (200 mg/kg BW) and maximum (400 mg/kg BW) received Dorema aucheri hydroalcoholic extract daily for 28 days orally. After 14 and 28 days all animals in the different groups were weighed and their blood collected, and SOD and GPX activities, and vitamin E and C concentrations were measured by validated standard methods. The results showed that in the short term, SOD and GPX activities, as well as vitamin C concentration increase depending on the dose, but in the long term these parameters decrease proportional to the increase of the dosage. Vitamin E concentration had no significant changes during our study.

Key Words: Dorema aucheri hydroalcoholic extract, antioxidant enzymes, antioxidant vitamins

 

ÖZET

DOREMA AUCHERI HİDROALKOL EKSTRAKTININ İN VİVO KAN ANTİOKSİDAN ENZİMLERİ (SOD VE GPX) VE VİTAMİN (E VE C) DÜZEYLERİ ÜZERİNE ETKİSİ

Dorema aucheri, Apiaceae familyasından flavonoid ve kumarinler gibi çeşitli komponentleri içeren bir bitkidir. Flavonoidler antitümöral, antikanserojenik ve östrojenik özelliklerine sahiptir. Kumarinlerin antiandrojenik özellikleri de bilinmektedir. Bu araştırmanın amacı, Dorema aucheri hidroalkolik ekstraktlarının SOD ve GPX aktiviteleri ile E ve C vitaminlerinin serum konsantrasyonları üzerine etkilerini araştırmaktır. 60 adet, 220-220 g ağırlığında ergin Wistar sıçanı 15 adetlik 4 gruba ayrılmıştır. Kontrol grubuna herhangi bir ekstrakt verilmemiştir. Diğer üç deneme grubuna 28 gün boyunca ağız yoluyla, düşük (100 mg/kg CA), orta (200 mg/kg CA) ve yüksek (400 mg/kg CA) düzeyde Dorema aucheri hidroalkolik ekstraktı verilmiştir. Tüm gruplarda bulunan hayvanlar 14 ve 28 gün sonra tartılmış ve alınan kanlarından SOD ve GPX aktiviteleri ile vitamin E ve C konsantrasyonları valide edilmiş standart metodlar ile ölçülmüştür. Elde edilen sonuçlar, kısa vadede SOD ve GPX aktivitelerinin yanısıra vitamin C konsantrasyonunun doza bağlı olarak arttığını, ancak uzun vadede bu parametrelerin doz artışı ile orantılı olarak azaldığını göstermiştir. Çalışma esnasında E vitamini konsantrasyonu düzeylerinde anlamlı değişiklik gözlenmemiştir.

Anahtar Kelimeler: Dorema aucheri hydroalkolik ekstraktı, antioksidan enzimler, antioksidan vitaminler

Anahtar Kelimeler

• Dorema aucheri hydroalkolik ekstraktı, antioksidan enzimler, antioksidan vitaminler

Kaynakça

1. Afshoun, A., Mahdavi, R., Rahimi, A., Sadeghi, H., 20 Investigation of Dorema aucheri hydroalcoholic extract on mammalian tumor in rats. Journal of Armaghan Danesh 15, 224-232. Azarneuoshan, F., Karami, A.M., Gholizadeh, L., Davarik, L., 2010. The effect of dorema aucheri-hydroalcoholic extracts on thyroids hormones in adult male rats. Journal of the Shahrekord University of Medical Sciences 12, 84Blot, W.J., Li, J.Y., Taylor, P.R., 1993. Nutrition intervention trails in linxian China: Supplementation with specific vitamin mineral combination cancer incidence and disease specific mortality in the general population. Journal of the National Cancer Institute 85, 1483-1492.
2. Chan, J.M., Stampfer, M.J., Giovannucci, E.L., 19 What causes prostate cancer? A brief summary of the epidemiology. Seminars in Cancer Biology 8, 263-273. Clark, L.C., Combs, G.F., Turnbull, B.W., 1996. Nutritional prevention of cancer study group. Effect of selenium supplementation for cancer prevention in patients with carcinoma of skin: A randomized controlled trial. The Journal of the American Medical Association 276, 1957-1963. Combs, G.F., Gray, W.P., 1998. Chemopreventive agents: Selenium. Pharmacology and Therapeutics 79, 179-192.
3. Dietrichb, M., Traber, M.G., Jacques, P.F., Cross, C.E., Hu, Y., Block, G., 2006. Does ãtocopherol play a role in the primary prevention of heart disease and cancer? A review. Journal of the American College of Nutrition 25, 2922
4. Duffield-Lillico, A.J., Reid, M.E., Turnbull, B.W., Jr Combs, G.F., Slate, E.H., Fischbach, L.A., Marshall, J.R., Clark, L.C., 2002. Baseline characteristics and the effect of selenium supplementation on cancer incidence in a randomized clinical trial: a summary report of the nutritional prevention of cancer trial. Cancer Epidemiology, Biomarkers and Prevention 11, 630-6
5. Gonzales, R., Auclair, C., Voisin, E., Gautero, H., Dhermy, D., Boivin, P., 1984. Superoxide dismutase, catalase and glutathione peroxydase in red blood cells from patients with malignant diseases. Cancer Research 44, 4137-4139.
6. Hajipour, A., Fzilati, M., Vahabi, M.R., Mostafavi, S.H., 2009. Study the biological activates of Dourema aucheri boiss. 10 th Congress of Biochemistry and 3 th
7. International Congress of Biochemistry and Molecular Biology, TehranIran, pp.124. Halliwell, B., Gutteridge, J.M.C., 1999. Antioxidant defences. In: Halliwell, B., Gutteridge, J.M.C. (Eds.), Free Radicals in Biology and Medicine. 3 rd edition Oxford, Clarendon Press, pp. 2002
8. Heinonen, O.P., Albanes, D., Vitamino, J., 1998. Prostat cancer and supplementation with alphatocopherol and beta-carotene:incidence and mortality in a controlled trial. Journal of the National Cancer Institute 90, 440-446.

9. Holben, D.H., Smith, A.M., 1999. The diverse role of selenium within selenoproteins: A review. Journal of the American Dietetic Association 99, 836-843.
10. Jain, N.C., 1986. Schalm's Veterinary Hematology. 1 st edition, Lea & Febiger, Philadelphia. pp. 25-29. Kamyab Moghadas, B., Safekordi, A.A., Fathi Kaljahi, J., Honarvar, B., Vaziri, S.A., 2010. Experimental and modeling of supercritical extraction of flavonoid compounds from dorema aucheri boss. World Applied Sciences Journal 14, 84-91.
11. Kidd, P.M., 1997. Glutathione: Systemic protectant against oxidative and free radical damage. Alternative Medicine Review 2, 155-176.
12. Knekt, P., Reunanen, A., Jarvinen, R., Seppanen, R., Heliovaara, M., Aromaa, A., 1994. Antioxidant vitamin intake and coronary mortality in a longitudinal population study. American Journal of Epidemiology 139, 118011
13. Lee, I.M., Cook, N.R., Gaziano, J.M., Gordon, D., Ridker, P.M., Manson, J.E., 2005. Vitamin E in the primary prevention of cardiovascular disease and cancer: the Women's Health Study: a randomized controlled trial. Journal of American Medical Association 294, 56-65.
14. Lopaczyski, W., Zeisel, S.H., 2001. Antioxidants, programmed cell death and cancer. Nutrition Research 21, 295-307.
15. Mokhtari, M., Sharifi, A., Parang, A., 2008a. Investigation of Dorema aucheri hydroalcoholic extract on hematologic parameters in rats. Journal of the Zanjan University of Medical Sciences 16, 37-44.
16. Mokhtari, M., Shariati, M., Niknam, H., 2008b. The effect of antinociceptive and anti-inflammatory of hydroalcoholic extract of Dorema aucheri on formalin test and carrageenan model in Rats. Journal of the Rafsanjan University of Medical Sciences 7, 165-172.
17. Norusis, M.Y., 1993. SPSS for Windows Base System User’s Guide Release 6.0. 1 st edition, SPSS Inc Michigan. pp. 281-290.
18. Paglia, D.E., Valentine, W.N., 1967. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. Journal of Laboratory and Clinical Medicine 70, 158-169.
19. Sadeghi, H., Chaitasi, I., Khademzadeh, R., Mehraban, Z., Afshoun, E., 2007a. Evaluation the effect of Dorema aucheri on important biochemical factors in rat. International Comparative Research on Traditional Medicine 66, 17-19.
20. Sadeghi, H., Ghaitasi, I ., Marzooghi, N., Sabzali, S., 2007b. The hepatoprotective effects of Dorema aucheri on carbon tetracholoride induced liver damage in rats. Journal of the Shahrekord University of Medical Sciences 6, 38-43.
21. Salonen, J.T., Alfthan, G., Huttunen, J.K., Pikkarainen, J., Puske, P.P., 19 Association between cardiovascular death and myocardial infarction and serum selenium in a matched-pair longitudinal study. Lancet 2, 1751
22. Sen, C.K., Khanna, S., Roy, S., 2006. Tocotrienols: vitamin E beyond tocopherols. Life Sciences 78, 2088-2098.
23. Singh, S.K., Dua, T.D., Tondon, A., Kumari, S., Ray, G., Batra, S., 1999. Status of lipid peroxidation and antioxidant enzymes in hypoxic ischemic encephalopathy. Indian Pediatrics 26, 659-668.
24. Traber, M.G., Shils, M.E., Shike, M., Ross, A.C., Caballero, B., Cousins, R., 2006. Vitamin E In Modern Nutrition in Health and Disease. 10 th edition Baltimore, MD, Lippincott Williams & Wilkins, pp. 396-411. Traber, M.G., 200 Vitamin E regulatory mechanisms. Annual Review of Nutrition 27, 347-3
25. Verhagen, H., Buijsse, B., Jansen, E., Bueno-deMesquita, B., 2006. The state of antioxidant affairs. Nutrition Today 41, 244-250.
26. Weitberg, A.B., Corvese, D., 1997. Effect of vitamin E and beta-carotene on DNA strand breakage induced by tobacco-specific nitrosamines and stimulated human phagocytes. Journal of Experimental and Clinical Cancer Research 16, 11