-

Öz

Foot and mouth disease virus (FMDV) comes under genus Aphthovirus and family Picornaviridae and it causes a severe vesicular disease in cloven footed animals especially cattle. It has seven different serotypes but only four of them (O, A, Asia I, C) are prevalent in Pakistan. The present study was aimed to evaluate the prevalence of these serotypes in Punjab province, Pakistan. A total of 659 samples were collected from different outbreaks of FMD occurring in Punjab province from 2001 to 2010. The samples were analysed by complement fixation test by using commercially available hyper immune sera of 4 serotypes. Results showed that 234 samples were positive for FMDV out of which 178 (27.01%) samples were positive for O, 4 (0.60%) for A, 48 (7.28%) for Asia I and 4 (0.60%) for C from 659 number of samples

Anahtar Kelimeler

• Foot and mouth disease virus, foot and mouth disease, hyper immune serum, complement fixation test

Distribution of Serotypes of Foot and Mouth Disease Virus Among Different Outbreaks Occurring in Punjab Province, Pakistan (2001-2010)

Öz

ABSTRACT

Foot and mouth disease virus (FMDV) comes under genus Aphthovirus and family Picornaviridae and it causes a severe vesicular disease in cloven footed animals especially cattle. It has seven different serotypes but only four of them (O, A, Asia I, C) are prevalent in Pakistan. The present study was aimed to evaluate the prevalence of these serotypes in Punjab province, Pakistan. A total of 659 samples were collected from different outbreaks of FMD occurring in Punjab province from 2001 to 2010. The samples were analysed by complement fixation test by using commercially available hyper immune sera of 4 serotypes. Results showed that 234 samples were positive for FMDV out of which 178 (27.01%) samples were positive for O, 4 (0.60%) for A, 48 (7.28%) for Asia I and 4 (0.60%) for C from 659 number of samples

Key Words: Foot and mouth disease virus, foot and mouth disease, hyper immune serum, complement fixation test  

ÖZET

PAKİSTAN, PENCAP İLİNDE ORTAYA ÇIKAN FARKLI SALGINLARDA ŞAP HASTALIĞI VİRÜSÜNÜN SEROTİPLERİNİN DAĞILIMI (2001-2010)

Şap hastalığı virüsü Aphthovirus cinsi ve Picornaviridae ailesine ait olup, başta sığır olmak üzere çift tırnaklı hayvanlarda önemli veziküler hastalığın oluşumuna neden olmaktadır. Yedi farklı serotipi olmasına rağmen bunlardan sadece dördü (O, A, Asya I, C) Pakistan'da yaygındır. Bu çalışmada, Pakistan'da Pencap ilinde bu serotiplerin prevalansının belirlemesi amaçlanmıştır. 2001 ile 2010 yılları arasında Pencap ilinde meydana gelen farklı şap hastalığı salgınlarından toplam 659 örnek toplanmıştır. Örnekler, ticari olarak temin edilen 4 serotipe ait hiper immun serumlar kullanılarak, komplement fiksasyon testi ile incelendi. Sonuçlar 659 örneğin 234'ünin şap hastalığı virüsü yönünden pozitif olduğunu, 178'inin (%27,01) O serotipinde, 4'ünün (%0,60) A serotipinde, 48'inin (%7,28) Asya I serotipinde ve 4'ünün (%0,60) C serotipinde olduğunu göstermiştir.

Anahtar Kelimeler: Şap hastalığı virüsü, şap hastalığı, hiper immun serum, komplement fiksasyon testi

 

Anahtar Kelimeler

• Şap hastalığı virüsü, şap hastalığı, hiper immun serum, komplement fiksasyon testi

Kaynakça

1. Anjum, R., Hussain, M., Zahoor, A.B., Irshad, H., Farooq, U., 2006. Epidemiological analyses of foot and mouth disease in Pakistan. International Journal of Agriculture and Biology 8, 648-651.
2. Brooksby, J.B., Roger, J., 1957. In: Methods of Typing and cultivation of Foot and mouth disease viruses (Project 208 of OEEG), Paris, pp.
3. Brooksby, J.B., 1958. The virus of foot and mouth disease. Advances in Virus Research 5, 1-37.
4. Fida, H., Hamid, A., Bhutta, A.I., 1965. Some epidemiological information on FMD in west Pakistan. Proceeding of 17 th Pakistan Scientific Conference 6, pp. 17-18.
5. Forman, A.J., 1974. A study of foot and mouth disease virus strains by complement fixation. Journal of Hygiene 72, 397-405.
6. Iqbal, H., Ahmad, I., Farooq, T., Nayyar, A., Khan, A.R., 2011. Prevalence of foot and mouth disease in catlle and buffaloes in central Punjab, Pakistan. International Journal for Agro Veterinary and Medical Sciences 5, 444-446.
7. Jamal, S.M., Ahmed, S., Hussain, M., Ali, Q., 2010. Status of foot and mouth disease in Pakistan. Archives of Virology 155, 1487-1491.
8. Khan, N.A., Choudhry, S.A., Sattar, A., Hussain, A., Haq, M.Z., Hafeez, S.Q., Ahmad, M.M., 19 Typographical studies of foot and mouth disease virus in Pakistan (1962-1988). Pakistan Journal of Veterinary Research 2, 7-10.

9. King, A.M.Q., Brown, F., Christian, P., Hovi, T., Hyypia, T., Knowles, N.J., Lemon, S.M., Minor, P.D., Palmenberg, A.C., Skern, T., Stanway, G., 2000. Picornaviridae. In: van Regenmortel, M.H.V., Fauquet, C.M., Bishop, D.H.L. (Eds.), Virus Taxonomy: Classification and Nomenclature of Viruses: Seventh Report of the International Committee on Taxonomy of Viruses, Academic Press, London, pp. 657-678.
10. Kitching, R.P., Knowles, N.J., Samuel, A.R., Donaldson, A.I., 1989. Development of foot and mouth disease virus strain characterisation, a review. Tropical Animal Health and Production 21, 153-166.
11. Kitching, R.P., 1998. A recent history of foot and mouth disease. Journal of Comparative Pathology 118, 89-108.
12. Klein, J., Hussain, M., Ahmad, M., Afzal, M., Alexandersen, S., 2008. Epidemiology of foot and mouth disease in Landhi dairy colony, Pakistan, the world largest buffalo colony. Virology Journal 5, 53.
13. Knowles, N.J., Samuel, A.R., 2003. Molecular epidemiology of foot and mouth disease virus. Virus Research 91, 65-80.
14. Newman, J.F., Rowlands, D.J., Brown, F., 1973. A physico-chemical sub-grouping of the mammalian picornaviruses. Journal of General Virology 18, 171-180.
15. Rauf, A.M., Khan, N.A., Ahmad, M., 1981. Typographical study of FMD virus in Pakistan. Pakistan Veterinary Journal 1, 13-14.
16. Sutmoller, P., Barteling, S.S., Casas, O.R., Sumption, K.J., 2003. Control and eradication of foot and mouth disease. Virus Research 91, 101-1
17. Thomson, G.R., Vosloo, W., Bastos, A.D., 2003. Foot and mouth disease in wildlife. Virus Research 91, 145-161.
18. Thomson, G.R., 1994. Foot and mouth disease. In: Coetzer, J.A.W., Thomson, G.R., Tustin, R.C., Kriek, N.P.J. (Eds.), Infectious Diseases of Livestock with Special reference to Southern Africa, Oxford University Press, Cape Town, pp. 825-852.
19. Valee, H., Carre, H., 1922. Sur la pluralite des virus aphteux. Comptes Rendus de Academie des Sciences Paris 174, 1498-1500.
20. Waldmann, O., Trautwein, K., 2003. Experimentelle untersuchungen ueber die pluralitet des maulund klauenseuche virus. Berliner und Munchener tierarztliche Wochenschrift 91, 3-7. Zulfiqar, M., 2003. Draft report for development of national disease control policy for Foot and Mouth Disease in Pakistan under the FAO project “Support for emergency prevention and control of main trans-boundary animal diseases in Pakistan Rinderpest, FMD, PPR.