Effects of Tarantula Cubensis Extract on Electrocardiographic and Trace Element Status in Sheep

Öz

The purpose of this research was to investigate the acute effects of Tarantula cubensis extract (Theranekron, 1:100/D2, Richter Pharma, Austria) on some serum enzymes and trace elements, and the cardiovascular system in sheep. Seven sheep (age 6 months, 35±10 kg) were used in this study. Theranekron was applied in 6 ml doses subcutaneously. It was determined that in the serum Urea, Creatinin, ALT, ALP, G-GT, LDH, Ca and P levels were increased and T. protein, Mg, Cu, Fe, Zn and Se levels were decreased. The important results obtained after the treatment with Theranekron on cardiovascular system were blood pressure increases and some morphological changes in ECG. We concluded the Theranekron treatment can cause adverse effects in sheep and further investigation of the efficacy of Theranekron treatment is warranted.

Anahtar Kelimeler

• Tarantula cubensis ekstraktı, koyun, EKG, iz elementler

Tarantula Cubensis Ekstraktının Koyunlardaki Elektrokardiyografik Durum ve İz Elementler Üzerindeki Etkileri

Öz

Bu çalışmanın amacı Tarantula cubensis ekstratının (Theranekron, 1:100/D2, Richter Pharma, Avusturya) koyunlardaki bazı serum enzimleri, iz elementler ve kardiovasküler sistem üzerine olan etkilerini incelemektir. Bu çalışmada 7 koyun (6 aylık, 35±10 kg) kullanılmıştır. Theranekron 3 ml dozunda subcutan olarak uygulanmıştır. Serum üre, kreatinin, ALT, ALP, G-GT, LDH, Ca ve P seviyelerinin arttığı ve Total Protein, Mg, Cu, Fe, Zn ve Se seviyelerinin azaldığı belirlenmiştir. Threnekronla tedaviden sonra kardiovasküler system üzerine görülen önemli sonuçlar kan basıncının artması ve EKG üzerindeki değişimlerdir. Theranekron tedavisinin koyunlarda yan etkileri olabileceğine ve Theranekronun etkinliği üzerine daha fazla çalışma yapılması gerektiğine karar verilmiştir.

Anahtar Kelimeler

• Tarantula cubensis extract, sheep, ECG, trace elements

Kaynakça

1. Albay, M.K., Şahinduran, Ş., Kale, M., Karakurum, M.Ç., Sezer, K., 2010. Influence of Tarantula cubensis extract on the treatment of the oral lesions in cattle with bluetongue disease. Veteriner Fakültesi Dergisi 16 (4), 593-596. Kafkas Üniversitesi
2. Başoğlu, A., 2002. P-QRS-T’nin morfolojik bozuklukları. In: Veteriner Kardiyoloji. Edit. Başoğlu, A. Çağrı Basın Yayın, Ankara. pp. 90-107.
3. Boedler, C.R., 2002. The brown spider Loxosceles laeta: source of the remedy Tarantula cubensis? Homeopathy 91, 166-170.
4. Çam, Y., Kibar, M., Atasever, A., Atalay, Ö., Beyaz, L., 2007. Efficacy of levamisole and Tarantula cubensis venom for papillomatosis. Veterinary Record 160, 486-488.
5. Carlson, G.P., 1990. Clinical Chemistry Tests. In: Large Animal Internal Medicine. Edit by Smith, B.P. Third ed., Part IV, Mosby Company, USA, pp. 389-412.
6. Cook, C.J., 2002. Glucocorticoid feedback increases the sensitivity of the limbic system to stress. Physiology and Behavior 75, 455-464.
7. Dakka, A.A., Abdel-All, T.H.S., 1992. Studies on minerals picture in the blood sera of egyptian Sheep. Assiut Veterinary Medicine Journal 28, 242-249.
8. Fazio, E., Ferlazzo, A., 2003. Evaluation of stress during transport. Veterinary Research Communications 27, 519-524.

9. Gertsch, M., 2009. Electrolyte imbalance and disturbances, In: The ECG Manual: An Evidence-Based Approach. Springer, pp. 177-190.
10. Gültiken, N., Vural, M.R., 2007. The effect of Tarantula cubensis extract applied in pre and postoperative period of canine mammary tumours. Journal of Investigative Surgery 2, 13-23.
11. Kaçar, C., Zonturlu, A.K., Oral, H., Yıldız, S., Arı, U.Ç., 2007. İneklerde erken puerperal dönemde Theranekron uygulamalarının uterus involusyonu ve vaginal akıntı üzerine etkisi. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 13, 11-15.
12. Lotfollahzadeh, S., Alizadeh, M.R., Mohri, M., Dezfouli, M.R.M., 2012. The therapeutic effect of Tarantula cubensis extract (Therenekron) in foot and mounth disease in cattle: a randomized trial in an endemic setting. Homeopathy 101, 159-164.
13. Miller, M.S., Tilley, L., Smith, F., Fox, P., 1999. Electrocaridography in Textbook of Canine and Feline Cardiology. Edit. Fox, P., Sisson, D., Moise, S. Saunders Comp., USA pp. 67-106.
14. Pellitier, K.R., 2013. Homeopathy: like cures like http://www.drpelletier.com/TBAM/excerpts/084- Homeopathy_Like.html (Accessed 24.04.2014)
15. Sardari, K., Kakhki, E.G., Mohri, M., 2007. Evaluation of wound contraction and epithelialization after subcutaneous administration of Theranekron® in cows. Comparative Clinical Pathology 16, 197-200.
16. Wang, M., Liu, Q., Luo, H., Li, J., Tang, J., Xiao, Y., Liang, S., 2008. Jingzhaotoxin-II, a novel tarantula toxin preferentially targets rat cardiac sodium channel. Biochemical pharmacology 76, 1716-1727.
17. Weisinger, R.S., Blair-West, J.R., Burs, P., Denton, D.A., Purcell, B., Vale, W., Rivier, J., Weisinger, H.S., May, C.N., 2004. Cardiovascular effects of long-term central and peripheral administration of urocortin, corticotropin-releasing factor, and adrenocortico- tropin in sheep. Endocrinology 145, 5598-5604.