Examination of Vancomycin Resistant Enterococci (VRE) Isolated from Canine and Feline Rectal Swabs

Öz

The aim of this study was to determine the occurrence of vancomycin resistant enterococci (VRE) in dogs and cats, examine the antimicrobial resistance profiles phenotypically, and the distribution of the vancomycin resistance associated genes by PCR. For this purpose, rectal swabs from animals were collected and processed for VRE isolation. After the identification of the isolates, antimicrobial susceptibility of the isolates were determined according to the Clinical and Laboratory Standards Institute (CLSI) standards. Finally, distribution of the vancomycin resistance associated genes; vanA, vanB, vanC1 and vanC2/C3 were determined by PCR.  Totally 157 (86 canine, 71 feline) rectal swab samples were examined. VRE were isolated from 12 canine and 8 feline samples. The VRE isolation rate in companion animals was 12.7%. Antimicrobial susceptibility results varied among the isolates; however, enrofloxacin resistance was common in both species. Multidrug resistance was also detected. As a conclusion, screening of distribution of VRE among pet animals would be useful to detect any emerging antimicrobial resistance problem related to public health.

Kedi ve Köpek Rektal svablarından İzole Edilen Vankomisin Dirençli Enterokokların (VRE) Araştırılması

Öz

Bu çalışmanın amacı kedi ve köpeklerde vankomisin dirençli enterokokların (VRE) varlığının araştırılması, fenotipik olarak antimikrobiyal direnç profillerinin incelenmesi ve vankomisin direnci ile ilişkili genlerin dağılımının PCR ile saptanmasıdır. Bu amaçla kedi ve köpeklerden rektal sıvap örnekleri toplandı ve VRE izolasyonu yönünden incelendi. İzolatların identifikasyonunu takiben, Clinical and Laboratory Standards Institute (CLSI) standartlarına göre antimikrobiyal duyarlılıkları araştırıldı. Son olarak, vankomisin dirençle ilişkili genleri; vanA, vanB, vanC1 ve vanC2/C3 gen bölgeleri PCR ile saptandı. Toplam 157 (86 köpek, 71 kedi) sıvap örneği incelendi %12,7’ sinden VRE izole edildi. 12 köpek ve 8 kedi örneğinden VRE izole edildi. Antibiyotik duyarlılık sonuçları izolatlar arasında farklılıklar göstermekle birlikte tüm izolatlar arasında enrofloksasin direnci en sık karşılaşılan dirençti. Aynı zamanda çoklu antibiyotik direnci de saptandı. Sonuç olarak, pet hayvanlarında VRE dağılımının sürekli taranması, antibiyotik direnci ile ilişkili olası halk sağlığı problemlerinin önlenmesi açısından önemi vurgulandı.

Anahtar Kelimeler

• Vankomisin direnci, Enterococcus spp., evcil hayvanlar, rektal sıvap

Kaynakça

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