İstanbul Yöresinde Sağlıklı ve Klinik Belirti Gösteren Köpeklerde Helicobacter pylori Varlığının Kültür, Polimerase Chain Reaction (PCR) ve Restriction Fragment Length Polymorphism (RFLP) ile Saptanması

Yıl 2011, Cilt: 37 Sayı: 2, 149 - 159, 01.11.2011

Sonay Sağnak N. Yakut Özgür

Öz

Günümüzde Helicobacter pylori'nin dünya nüfusunun neredeyse yarısını infekte eden bir patojen olması ve gün geçtikçe yayılması, kedi ve köpeklerin insanlar için infeksiyon kaynağı olabileceğini düşündürmekte, bu konuda araştırmalara yönlenmeye gereksinim oluşturmaktadır. bu araştırmada, köpeklerde de insanlardakine benzer infeksiyona neden olabileceği düşünülen H. pylori'nin varlığı köpek dışkı örneklerinde araştırılmıştır. Bu amaçla İstanbul'da bulunan toplam 96 adet pet ve sokak köpeğine ait dışkı örnekleri toplanmıştır. Dışkı örnekleri Polymerase Chain Reaction (PCR) ile incelenmiş, Helicobacter DNA'sı saptanan örneklerde H. pylori'de bulunan CagA geninin saptanması amacıyla Restriction Fragmenth Length Polymorphism (RFLP) ile H.pylori spesifik bandlar araştırılmıştır. Ayrıca çalışmada PCR-RFLP ile H. pylori saptanan dışkı örneklerinden bakteriyolojik kültür yapılarak moleküler yöntemler ile kültür tekniği de karşılaştırılmıştır. Helicobacter cins spesifik primerler kullanarak yapılan PCR uygulaması sonucu 96 örneğin 65 (% 67,7) inden elde edilen 375 bp band sonuçlarını takiben HinfI enzim kesimi ile 12 (% 18,46) DNA örneğinde H.pylori spesifik bandlar (196 bp, 119 bp, 60 bp) saptanmıştır. H. pylori DNA'sı saptanan 12 köpeğin dışkı örneğinden yapılan bakteriyolojik inceleme sonucunda ise örneklerin hiçbirinden H. pylori izole edilememiştir.

Anahtar Kelimeler: Köpek, Helicobacter pylori, PCR, RFLP, kültür

Anahtar Kelimeler

Köpek, Helicobacter pylori, PCR, RFLP, kültür

Kaynakça

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