VAKUM PAKETLİ SIGIR ETLERİNİN BOZULMA ETKENİ Clostridium Estertheticum YÖNÜNDEN PCR İLE ARAŞTIRILMASI

Yıl 2009, Cilt: 35 Sayı: 1, 1 - 6, 20.11.2009

Aysun Yılmaz Christopher Helps Gozde Kaya Erkan Kırat

Öz

 

 

INVESTIGATION on the PRESENCE of SPOILAGE BACTERIA Clostridium estertheticum in VACUUM-PACKED BEEF by PCR 

 

Abstract

One of the psychrophilic Clostridia , Clostridium estertheticum has been recognized as causative agents of "blown pack" spoilage of vacuum-packed chilled (0-4°C) meats indicative of odor and vacuum pack distension. Therefore, this stuy was planned to investigate the presence of potential spoilage bacteria, Clostridium estertheticum in vacuum-paked beef. For this, DNA samples from 70 vacuum-packed beef from Istanbul, Turkey were analysed for the presence of Clostridium estertheticum DNA by PCR. No targeted Clostridium estertheticum DNA was found in the samples. In conclusion, although no positivity for Clostridium estertheticum found in this study, it should be monitored in meat sectory whether this bacteria causes spoilage in vacuum-packed beef in Turkey or not. In this study, presence of Clostridium estertheticum was investigated by PCR for the first time in Turkey and the results will aid scientific infomation and background to meat sector).

 

Key Words: Clostridium estertheticum, beef, vacuum-packed, PCR

 

 

 

VAKUM PAKETLİ SIGIR ETLERİNİN BOZULMA ETKENİ Clostridium estertheticum YÖNÜNDEN PCR İLE ARAŞTIRILMASI

 

Özet

Psikrofil klostridiumlardan Clostridium estertheticum vakum paketli soğukta (0-4°C) tutulan etlerde bozulmalara ve dolayısıyla koku ve bombaja (blown pack) neden olmaktadır. Bu nedenle bu çalışmada, vakum paketli etlerde potansiyel bozulmaya neden olabilecek Clostridium estertheticum varlığının araştırılması amaçlandı. Bu amaca yönelik olarak, değişik firmalardan temin edilen 70 vakum paketli sığır eti Clostridium estertheticum DNA'si yönünden PCR ile incelendi. İncelenen DNA Örneklerinde hedeflenen Clostridium estertheticum DNA'si saptanmadı. Sonuç olarak çalışmada vakum paketli sığır etlerinde Clostridium estertheticum saptanmamış olsa da etkenin ülkemizde et sektöründe problem oluşturup oluşturmadığı izlenmelidir. Bu çalışma ile etken PCR ile ülkemizde ilk kez araştırılarak sektöre ve araştırıcılara bilimsel katkı sağlanmaya çalışılmıştır.

Anahtar Kelimeler: Clostridium estertheticum, sigir eti, vakum paket, PCR

 

Anahtar Kelimeler

Clostridium estertheticum, sığır eti, vakum paket, PCR

Kaynakça

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• Aysun Y I L M A Z - Christopher HELPS - Gözde K A Y A - Erkan KIRAT
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