ISOLATION OF Mannheimia haemolytica FROM THE UHT MILK

Yıl 2009, Cilt: 35 Sayı: 1, 49 - 53, 20.11.2009

Aysun Yılmaz Gözde Dalkılıç Kaya

Öz

 

ABSTRACT

This study describes the isolation and identification of Mannheimia haemolytica from the UHT milk. This is important for public health since human might get infected with this bacteria. Three package of semi-skimmed UHT drinking milk with cacao was obtained from a commercial company and analysed in a private laboratory (Istanbul, Turkey) bacteriologically. The gross apparence of the milk was not normal. It was thickened and clotted. Milk homogenates were analysed by using PCA and sheep blood agar. After Gram's staining Gram negative coccobasils were observed. Pasteurella pneumotropical Mannheimia haemolytica was identified according to the API 20E.

Key Words: Isolation, Mannheimia haemolytica, UHT milk

 

UHT SÜTTEN Mannheimia haemolytica İZOLASYONU

ÖZET

Bu çalişma ile UHT sütten Mannheimia haemolytica izolasyonu ve identifikasyonu tanımlandı. Etken insanlarda da infeksiyona neden olduğu için önemlidir. Bu calışmada, 2008 yılında ticari bir firmadan temin edilen yarı-yağlı, 3 paket UHT sütün bakteriyel analizi yapıldı. Sütte organoleptik açıdan anormal görünüm ve pıhtı saptandı. Süt homojenize edildikten sonra PCA ve koyun kanlı agara ekimler yapıldı. Gram boyama sonucunda Gram negatif kokobasiller görüldü ve etken API-20E identifikasyon sistemi kullanılarak Pasteurella pneumotropical Mannheimia haemolytica olarak identifiye edildi.

Anahtar Kelimeler: İzolasyon, Mannheimia haemolytica, UHT süt

Anahtar Kelimeler

İzolasyon, Mannheimia haemolytica, UHT süt

Kaynakça

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