HOROZLARDA SEMİNAL PLAZMA LİPİD PEROKSİDASYONU VE EKSTRASELÜLER ANTİOKSİDANLAR İLE BAZI SPERMATOLOJİK ÖZELLİKLER ARASINDAKİ İLİŞKİLER

Yıl 2005, Cilt: 31 Sayı: 1, 67 - 74, 08.03.2013

Pınar Ulutaş İlker Serin Ahmet Ceylan

Öz

ÖZET

Bu çalışmada horozlarda seminal plazmadaki lipid peroksidasyonunun göstergesi olan malondialdehit (MDA), ekstraseluler antioksidanlardan seruloplazmin ve ürik asit düzeyleri ile sperma yoğunluğu, ölü-canlı spermatozoon oranı ve toplam anormal spermatozoon oranları arasındaki ilişkinin saptanması amaçlanmıştır.

Çalışmada toplam 24 sperma örnegi analiz edilmiştir. Bu örnekler 10 horozdan 3 gün arayla 3 kez kloakanın masajı yöntemi ile elde edilmiştir. Sperma örneklerinde yoğunluk, ölü ve canlı spermatozoon oranı ve toplam anormal spermatozoon oranı ile seminal plazmalarda MDA, seruloplazmin ve ürik asit düzeyleri belirlenmiştir. Sonuçlar basit korelasyon analizi ile değerlendirilmiştir. Ortalama seminal plazma seruloplazmin konsantrasyonu ile toplam anormal spermatozoon oranı arasında istatistiksel açıdan onemli (r= 0,543, p<0.05) pozitif korelasyon bulunurken diğer parametreler arasında istatistiksel önemde bir ilişkiye rastlanmamıştır.

Anahtar Kelimeler: Seminal plazma, MDA, seruloplazmin, ürik asit, horoz

 

ABSTRACT

THE RELATIONSHIP BETWEEN SEMINAL PLASMA LIPID PEROXIDATION AND EXTRACELLULER ANTIOXIDANTS WITH SOME SPERMATOLOGICAL FEATURES IN COCK SEMEN.

In this study, we aimed to determine the relationship between MDA levels in seminal plasma that indicates lipid peroxidation and sperm concentrations of extracellular antioxidants such as ceruloplasmin and uric acid, with rates of alive and dead spermatozoa and the spermatozoa morphology in cocks.

24 semen specimens were analysed in this study. These samples were obtained from ten cocks by cloacal stimulation within 15 days period. MDA, ceruloplasmin and uric acid levels was measured in seminal plasma and sperm concentration, rates of alive and dead spermatozoa and morphology in semen specimen. Results were investigated with basic correlation analyses. Statistically significant positive correlation (r= 0.543. p<0.05) was determined between mean seminal plasma ceruloplasmin concentration with spermatozoon morphology; however no significant correlation between other parameters were determined.

Key words: Seminal plasma, MDA, ceruloplasmin, uric acid, cock.

 

Anahtar Kelimeler

Seminal plazma, M D A , seruloplazmin. ürik asit. horoz

Kaynakça

• Agervald, A., R a m a d a n A. S., Saleh, M . D . , Bedaivvy M . A . Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril, 2002: 79 (4) 829-843.
• Aksoy, H . , Aksoy, Y . , Ö z b e y L , A l t u n t a ş , I, A k ç a y F . The relationship between varicole and semen nitric oxide concentrations. Urol Res, 2000; 28:357-359.
• Aitken, R . J . C l a r k s o n J S , Fİshel S. Generation of reactive oxygen species l i p i d peroxidation, and human sperm function. B i o l Reprod. 1989; 40: İ 8 3 - 1 9 7 .
• Aitken, R . J . , West, K . M . Analyses o f the relationship betvveen reactive oxygene species production and leukocyte infiltration in fractions o f human semen separated on percoll gradients. Int J A n d r o l , 1990; 3:433-451.
• Blank, İL, Hofstetter, A. Complement C3. coeruloplasmin and PMN-elastase in the ejaculate in chronic prostato-adnexitis and their diagnostic value. Infection, 1991; 3: 138-140
• Busch,H.V., L ö h l e , K . , Peter, W . Künstliche Besamung bei Nutzicien. 2. Auflage. Gustav Fischer Verlag. Jena-Stutgart 1991. K ü n s ü c h e Besamung beim geflügel. 601-632
• Burtis, C . A . , Ashvvood, E . R . Tietz Textbook of Clinical Chemistry. 3r d Edition. Chapter 20.477-507. W . B . Sounders Company. 1999.
• Chaiching, L . , C h i a C h e r i n g , H . , Mingcheng, C , Jengfang, F L , Hungyi, C , L i n , C . C . , Huang, C . C . , Chen, M . C . , Huang, J . F . A . , Chiou, H . Y . Arsenic toxicity on duck spermatozoa and the ameliorating effect o f L - ascorbic acid. Asian Ausralasian J A n i m Sci, 2002; 15(1): 19-25. Horozlarda Seminal Plazma Lipid Pcroksidasyonu ve Ekstraselülcr Antioksidanlar ile 73
• Ciereszko, A-, D o b r o v s k i , K . , K u c h a r c z y k , D . , Goryczko S., Glogovvski, J . The presence o f uric acid, an antioksidantive substance, in fish seminal plasma. Fish Physiol Biochem. 1999; 2 1 : 313-315.
• G r u y s , E . , Oblovvo, M . J . , Toussaint, J . M . Diagnosis significanse o f majör acute phase proteins in veterinary clinical ehemistry: a review. Vet B u l l , 1994; (64) 11: 1009 - 1015.
• G ü n a y , Ü., Polat, Ü., Soylu, M . K . , K i l , F . Alman Ç o b a n köpeklerinin seminal p l a z m a s ı n d a k i bazı biyokimyasal parametrelerin saptanması. T ü r k J Vet Sci, 2003; 27: 983-986.
• K e s k i n , O . , T e k i n , N., A k ç a y , E . Erbro ırkı horoz spermalarının farklı sulandırıcı ve kryoprotektanlarla d o n d u r u l m a s ı . Lalahan hay A r ş Ens Derg. 1995; 35(3-4): 110-115
• K r u s e , W . E . , Boit, R . , R o h r , G . , Aufenanger, J . , H u n d , M . , Strowitzki, T . Relationship o f seminal plasma interteukin-8 and I L - 6 vvith semen quality. Human Reprod, 2 0 0 1 ; 16 (3). 517- 528.
• Lewis, S . E . M . , Sterling, E . S . L . , Young, I . S . , Thompson, W . Comparison o f indivual antioxidants o f sperm and seminal plasma in fertile and infertile man. Fertik Steril, 1997; 67: 142-147.
• Motchnik, P . A . , F r e i , B . , Ames B . N . Measurements o f human antioxidants in human blood plasma. M e t h E n z y m o l . 1992;234:249-252.
• Nakamura, H . , K i m u r a , T . , Nakajima, A . , Shimoya, K , T a k e m u r a , M . , Hashimoto, K . , Isaka, S., A z u m a , C . , Koyoma, M . , M u r a t a , Y . Detection o f oxidative stres in seminal plasma and fractionated sperm from subfertile male patients. Eur J Obstet Gynecol Reprod, 2002: 155>160.
• O m u , A . E . , Al-Qattan, F . , Al-Abdulhadi, F . M . , Fatinikun, M . T . , Fernandes, S. Seminal immun response in fertile man with leukocytospermia : effect on antioxidant aetivity. Eur J Obstet Gynecol Reprod, 1999; 86:195-202.
• Orlando, C . , Caldini A . L . , B a r n i , T . , Wood, W . G . , Strasburger, C . J . , Natali, A . , M a v e r , A . , Forti, G . , Serio, M . Ceruloplasmin and transferin in human seminal plasma: are they an index o f seminiferous tubuler funetion. Fertil Steril, 1985; 43 (2): 290-294.
• Padron, O . F . , Brackett, N.I., S h a r m a R . K . , K o h n , S., Lynne, C . M . , Thomas, A . J . Seminal reactive oxygene species, sperm motility and morphology in man vvith spinal cord injury. Fertil steril,1997; 67: 1115-1120
• Saleh, R . A . , Agarvval, A . , Nada, E . A . , E l - T o n s y , M . H . , Sharma, R . K . , Nelson D . R . , Thomas, A . J . Negative effect o f inereased sperm D N A damage in relation to seminal oxidative stress in man vvith idiopathic and male factor infertility. Fertil Steril, 2003; 79: 1597-1605.
• Pınar Alkım ULUTAŞ - İlker SERİN - Ahmet CEYLAN
• 1 . Sunderman, F . W . , Nomoto, S. Measurements o f human serum ceruloplasmin by its p-phenylenediamine oxidase activity. C l i n Chem. 1970; 16: 903-910.
• S u r a i , P., Kostjuk, I . , Wishart, G . , Macpherson, A . , Speake, B . , \ o b l e , R . , Ionov, I . , K u t z , E . Effect o f vitamin E and selenium supplementation o f cockerel diets on glutathione peroxidase activity and l i p i d peroxidation susceptibility in sperm, testes. and iiver. B i o l Trace elements, 1998; 64(1-3): 119-132.
• S u r a i P . E . , Blesbois, E . , G r a s e a u , L , C h o l a h , T . , B r ü t a r d J . P . , \ Y i s h a r d , G . J . , Cerolini, S., Sparks, N . H . C . Fatty acid composition, glutathione peroxidase activity and total antioxidant activity o f avian semen. Comp Biochem phvsiol Part B . 1998; 120: 527-533.
• T e k i n , N . Spermanın muayenesi ve d e ğ e r l e n d i r i l m e s i . In: Evcil hayvanlarda R e p r o d ü k s i y o n , Suni tohumlama, d o ğ u m ve İnfertilite. Ed. A l a ç a m . E.. Konya. 1994, 69-79.
• Xue, C . , Y u , G . , Hirata, T . , Sakaguchi, M . , Terao, J . Antioxidative activity o f carp blood plasma on lipid peroxidation. Biosci Biotech Biochem, 3998; 62: 201ı 205.
• Yoshoiko, T . , Kavvada, K . , Shimada, T . L i p i d peroxidation in maternal and cord blood and protective mechanism against active-oxygen toxicity in the blood. A m J Obstet Gynecol, 1979; 135: 372-376.