Effects of Curcumin on Apoptosis in “In Vivo” Solid Ehrlich Ascites Tumor Model in Balb-C Mice

Yıl 2014, Cilt: 40 Sayı: 2, 183 - 190, 22.07.2014

Elif Ikıtımur-armutak Mehmet Gurtekın

Öz

Curcumin is the yellow pigment in turmeric (Curcuma longa L) that is widely used as a spice, food coloring (curry) and preservative. Curcumin has a wide range of pharmacological properties including anti-inflammatory, anti-tumor promoter and anti-oxidant effects. In this study, the development and quantity of apoptosis in tumor tissue of solid Ehrlich Ascites Tumor model developed balb-c mouse by applying 100 mg/kg curcumin in different time periods were investigated. For this aim; 54 Balb-c female mice, constituted in vivo tumor by subcutaneous injection of 3x10⁶ EAT cells, were divided into 3 experimental and 3 control groups. Using gavage method, 100 mg/kg curcumin dissolved in 9% alcohol was applied to experimental groups, 9% alcohol was applied to control groups in different time intervals. Long and short diameters measurement, and Terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin Nick End Labeling (TUNEL) and hematoxylin-eosin staining were applied to the extracted tumors of all groups and findings were supported with electron microscopic (TEM) evaluations. In long and short diameter measurement of acquired tumors, no significant differences were obtained (P>0.05). In microscopic evaluations different results were obtained from cell count of all groups. More apoptotic cells were determined in experimental group 2 (P<0.001) and 3 (P<0.05) than their control groups. According to statistical analysis, there was no significant difference between experimental group 1 that received longest-term curcumin and control 1 (P>0.05). Overall, the long-term 100 mg/kg applications of curcumin do not have any effect on apoptosis.

Key Words: Apoptosis, curcumin, ehrlich ascites tumor, mice

Anahtar Kelimeler

Apoptosis, curcumin, ehrlich ascites tumor, mice

EHRLICH ASCITES TÜMÖRÜ İLE BALB-C FARELERDE OLUŞTURULMUŞ SOLİD TÜMÖR MODELİNDE CURCUMİNİN APOPTOZ ÜZERİNE ETKİLERİ

Öz

Curcumin, turmeric (Curcuma longa L) de bulunan, yaygın olarak baharat, yiyecek reklendirici ve koruyucu olarak kullanılan sarı renkli pigmentdir. Curcumin anti-inflamatuar, anti-tümöral ve anti-oksidan gibi çeşitli farmakolojik özelliklerinden dolayı yaygın bir kullanıma sahiptir. Bu çalışmada, Balb-c farelerde geliştirilen Ehrlich Ascites Tümör modelinde farklı periyotlarda 100 mg/kg curcumin uygulamasının apoptosis miktarı ve gelişimi üzerine etkisi incelendi. Bu amaçla; 54 adet Balb-c dişi fare, in vivo tumor oluşturmak için 3x10⁶ EAT hücresinin subkutan olarak enjeksiyonunu takiben 3 deney ve 3 kontrol grubuna ayrıldı. Gavaj metodu kullanılarak %9 alkol içerisinde çözündürülen 100 mg/kg curcumin deney gruplarına, %9 alkol kontrol gruplarına farklı zaman aralıklarında uygulandı. Tüm gruplardan alınan tümörlerde kısa ve uzun çap ölçümleri, Terminal deoksiribonükleotidil trasferaz-aracılı dUTP-digoksigenin uç işaretleme tekniği (TUNEL) ve hematoksilen-eosin boyama uygulandı. Bulgular ayrıca elektron mikroskobik (TEM) değerlendirmelerle desteklendi. Tümörlerden elde edilen kısa ve uzun çap uzunluklarında herhangi bir farklılık görülmedi (P>0,05). Tüm gruplardan elde edilen hücre sayımlarında mikroskobik değerlendirmelerde farklı sonuçlar gözlendi. Deney Grubu 2 (P<0,001) ve 3 (P<0,05)’de ve Kontrol Gruplarına gore daha fazla apoptotik hücre tespit edildi. İstatistiksel verilere göre, uzun sure curcumin uygulaması yapılan Deney Grubu 1 ve bu gruba ait Kontrol 1 grubu arasında anlamlı bir fark yoktu (P>0,05). Genel olarak, Curcumin’in uzun sure 100 mg/kg uygulamasının apoptoz üzerinde herhangi bir etkisi yoktur.

Anahtar Kelimeler: Apoptoz, curcumin, ehrlich ascites tümör, fare

Anahtar Kelimeler

Apoptoz, curcumin, ehrlich ascites tümör, fare

Kaynakça

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