ANAL KESE BEZİ ADENOKARSİNOMALARININ KANTİTATİF MORFOLOJİ YÖNTEMİ İLE SUBTİPLENDİRİLMESİ

Yıl 2014, Cilt: 40 Sayı: 2, 243 - 248, 22.07.2014

Radostin Sımeonov

Öz

Bu retrospektif çalışmada, köpeklerde histopatolojik olarak doğrulanmış anal kese bezi adenokarsinomalarının nükleer morfometrik parametreleri sitolojik smear yöntemiyle incelenmiş ve neoplastik subtiple karşılaştırılmıştır. Morfometrik parametreler ve histopatolojik subtip arasındaki korrelasyon, önem derecesi P<0,05 düzeyinde Mann-Whitney U testi (Statistica 6,0, StatSoft, USA) kullanılarak belirlenmiştir. Tubüler, rozet ve kistik anal adenokarsinoma çeşitlerinin çalışılan tüm morfometrik parametreleri arasında istatistiki açıdan önemli farklılıklar bulunmuştur. Bu ön çalışmanın sonucu olarak nükleer morfometri yönteminin köpeklerde görülen anal kese bezi adenokarsinomalarının subtiplendirilmesi amacıyla yardımcı bir yöntem olarak kullanılabileceği söylenebilir.

Anahtar Kelimeler: Sitoloji, görüntü analizi, nükleer morfometri, histopatolojik subtipler, kanin anal kese bezi adenokarsinomaları

Anahtar Kelimeler

Sitoloji, görüntü analizi, nükleer morfometri, histopatolojik subtipler, kanin anal kese bezi adenokarsinomaları

Subtyping Canine Anal Sac Gland Adenocarcinomas by Means of Quantitative Morphology

Öz

In this retrospective study, nuclear morphometric parameters of histopathologically confirmed anal sac gland adenocarcinomas were investigated on cytological smears in dogs and compared with the neoplastic subtype. The correlation between morphometric parameters and histopathological subtype was determined using Mann-Whitney U test (Statistica 6.0, StatSoft, USA) at level of significance P<0.05. There were statistically significant differences between all studied morphometric parameters of tubular, rosette and solid anal adenocarcinoma patterns. The results of this preliminary study indicated that nuclear morphometry could be used as an auxiliary method for subtyping canine anal sac gland adenocarcinomas.

Key Words: Cytology, image analysis, nuclear morphometry, histopathological subtypes, canine anal sac gland adenocarcinomas

Anahtar Kelimeler

Cytology, image analysis, nuclear morphometry, histopathological subtypes, canine anal sac gland adenocarcinomas

Kaynakça

• Baak, J., 1991. Morphometric and combined morphometric-DNA Cytometric applications, the female reproductive tract ovary. In: Baak J, (Ed), Manual of quantitative pathology of Cancer Diagnosis and Prognosis, SpringerVerlag, Heidelberg, pp. 345-352.
• Baak, J., Tosi, P., 1991. Historical background of quantitative pathology. In: Baak J, (Ed). Manual of quantitative pathology of Cancer Diagnosis and Prognosis, Springer-Verlag, Heidelberg, pp. 3-6
• Bennet, P., De Nicola, D., Bonney, P., Glickman, N., Knapp, D., 200 Canine anal sac adenocarcinomas: clinical presentation and response to therapy. Journal of Veterinary Internal Medicine 16, 100-104. Bierhoff, E., Appel, K., Appel, T., Buetter, R., Wandemarin, E., 200 Comparative morphometric analysis of primary versus recurrent basal cell carcinoma and of histological subtypes. Significant of morphometry of the nuclei. Anticancer Research 23, 2697-2700.
• Brisson, B., Whiteside, D., Holmberg, D., 2004. Metastatic anal sac adenocarcinoma in a dog presenting to acute paralysis. Canadian Veterinary Journal 45, 678-681.
• Goldschmidt, M., Dunstan, R., Stannard, A., von Tscharner, C., Walder, E., Yager, J., 1998. Histological classification of Tumours of the Skin of Domestic Animals, World Health Organization International Classification of Tumors in Domestic Animals, Second Series, Vol III. Washington D.C.: Armed Forces Institute of Pathology, American Registry of Pathology.
• Goldschmidt, M., Shofer, F., 1993. Skin Tumours and the Dog and Cat: Butterworth Heinemann, Oxford.
• Goldschmidt, M., Zoltowski, C., 1981. Anal sac gland adenocarcinoma in the dog: 14 cases. Journal of Small Animal Practice 22, 119-128.
• Goldshmidt, M., Hendrick, M., 2002. Tumours of the Skin and Soft Tissue. In: Meuten, D., (Ed.), Tumors in Domestic Animals, 4 th ed., Iowa State Press; pp. 74-76.
• Hamilton, P., Allen, D., 1995. Morphometry in histopathology. Journal of Pathology 175, 3693
• Maiolino, P., Cataldi, M., Paciello, O., Restucci, B., De Vico, G. , 2005. Nucleomorphometric analysis of canine cutaneous mast cell tumours. Journal of Comparative Pathology 133, 2092
• Maiolino, P., Restucci, B., Papparella, S., 2002. Nuclear morphometry in squamous cell carcinomas of canine skin. Journal of Comparative Pathology 127, 114-117.
• Meuten, D., Cooper, B., Capen, C., Chew, D., Kociba, G., 1981. Hypercalcemia associated with an adenocarcinoma derived from the apocrine glands of the anal sac. Veterinary Pathology 18, 454-471.
• Ross, J, Scavelli, T., Mathiesen, D., 1991. Adenocarcinoma of the apocrine glands of the anal sac: A review of 32 cases. Journal of the American Animal Hospital Association 27, 3493
• Simeonov, R., 2009. Nuclear morphometry in relation to tumour grade in canine spontaneous cutaneous squamous cell carcinomas. Turkish Journal of Veterinary and Animal Science 33 (5), 439-442. Simeonov, R., Simeonova, G., 200 Nucleomorphometric analysis of feline basal cell carcinomas. Research of Veterinary Science 84, 440-443.
• Simeonov, R., Simeonova, G., 2008. Quantitative analysis in spontaneous canine anal sac gland adenomas and carcinomas. Research of Veterinary Science 85, 559-560.
• Simeonov, R., Simeonova, G., 2009. Nuclear cytomorphometry in feline mammary gland epithelial tumours. Veterinary Journal 179 (2), 296-300.
• Strefezzi, F., Xavier, J., Catao-Dias, J., 2003. Morphometry of canine cutaneous mast cell tumors. Veterinary Pathology 40, 268-275.