BibTex RIS Kaynak Göster

Evaluation of Protective Effect of Penicillamine on Silver Nanoparticles-Induced Oxidative Stress in BALB/c Mice

Yıl 2015, Cilt: 41 Sayı: 2, 205 - 211, 01.07.2015

Öz

Despite the negative effects of silver nanoparticles, their application is growing rapidly, so this study was designed to investigate the adverse effects of nanosilver administration on histopathological changes and biochemical parameters as well as therapeutic efficacy of penicillamine. Four groups of six BALB/c mice were treated intraperitoneally with various dose of nanosilver and the LD50 was calculated. To investigate therapeutic efficacy of penicillamine, forty-two mice were assigned into equal seven groups. The animals of each group were treated intraperitoneally with LD50 of nanosilver and 50 or 200 mg/kg penicillamine concurrently, or 4 hours after nanosilver injection. Histopathological and serum biochemical analyses including total protein, albumin, total globulin, cholesterol, triglyceride, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total antioxidant capacity (TAC) and Malondialdehyde (MDA) were carried out. The LD50 was 1500 PPM. Results showed an increase in the levels of AST and ALT enzymes and the MDA in the mice receiving nanosilver while TAC concentration decreased. These alterations were statistically significant between the mice receiving nanosilver and those receiving nanosilver and 200 mg/kg penicillamine. Also, histopathological examination of the tissue sections of the the mice receiving 1500 PPM nanosilver showed hepatic and renal damage. Whereas, the biochemical parameters and tissue lesions improved in mice receiving 200 mg/kg penicillamine in comparison with the nanosilver exposed mice and it was shown that the extent of oxidative stress was decreased.

Kaynakça

  • Akradi, L., Sohrabi-Haghdoost, I., Djeddi, A., Mortazavi, P., 2012. Histopathologic apoptotic effect of nanosilver in liver of broiler chickens. African Journal of Biotechnology 11, 6207-6211.
  • Beattie, A.D., 1977. The use of D-penicillamine for lead poisoning. Proceedings of the Royal Society of Medicine 70(Suppl 3), 43-45.
  • Burtis, C.A., Ashwood, E.R., 1994. Textbook of Clinical Chemistry, 2nd edn. Saunders, Philadelphia, pp 735- 888.
  • Buzea, C., Pacheco, I.I., Robbie, K., 2007. Nanomaterials and nanoparticles: sources and toxicity. Biointerphases 2(4), MR17-MR71.
  • Choi, J.E., Kim, S., Ahn, J.H., Youn, P., Kang, J.S., Park, K., Yi, J., 2010. Induction of oxidative stress and apoptosis by silver nanoparticles in the liver of adult zebrafish. Aquatic Toxicology 100, 151-159.
  • Choi, S.C., 1990. Interval estimation of the LD50 based on an up-and-down experiment. Biometrics 46(2), 485-92.
  • Hsin, Y.H., Chen, C.F., Huang, S., Shih, T.S., Lai, P.S., Chueh, P.J., 2008. The apoptotic effect of nanosilveris mediated by a ROS- and JNK- dependent mechanism involving the mitochondrial pathway in NIH3T3 cells. Toxicology Letters 179, 130-139.
  • Hussain, S.M., Javorina, A.K., Schrand, A.M., Duhart, H.M., Ali, S.F., Schlager, J.J., 2006. The interaction of manganese nano-particles with PC-12 vells induces dopamine depletion. Toxicological Sciences 92, 456-463.
  • Ji , J.H., Jung, J.H., Kim, S.S., Yoon, J.U., Park, J.D., Choi, B.S., 2007. Twenty-eight-day inhalation toxicity study of silver nanoparticles in Sprague-Dawley rats. Inhalation Toxicology 19, 857-871.
  • Kim, S., Choi, J.E., Choi, J., Chung, K.H., Park, K., Yi, J., Ryu, D.Y., 2009. Oxidative stress-dependent toxicity of silver nanoparticles in human hepatoma cells. Toxicology in Vitro 23, 1076-1084.
  • Kim, W.Y., Kim, J., Park, J.D., Ryu, H.Y., Yu, I.J., 2009. of Histological accumulation of silver nanoparticles in kidneys of Fischer 344 rats. Journal of Toxicology and Environmental Health A 72, 1279-1284. in
  • Klaine, S.J., Alvarez, P.J., Batley, G.E., Fernandes, F., Handy, R.D., Lyon, D.Y., Mahendra, S., Mclaughlin, M.J., Lead, J.R., 2008. Nanomaterials in the environment: behavior, Environmental Toxicology Chemistry 27, 1825-1851. and effects.
  • Luoma, P.V., 2008. Cytochrome P450 and gene activation-from pharmacology to regression of atherosclerosis. European Journal of Clinical Pharmacology 64, 841-850. elimination and
  • Lykkesfeldt, J., 2001. Determination of malondialdehyde as dithiobarbituric acid adduct in biological samples by HPLC with fluorescence detection: comparison with ultraviolet-visible Chemistry 47, 1725-1727. Clinical
  • Machiedo, G.W., Powell, R.J., Rush, Jr.B.F., Swislocki, N.I., Dikdan, G., 1989. The incidence of decreased red blood cell deformability in sepsis and the association with oxygen free radical damage and multiple system organ failure. Archives of Surgery 124, 1386-1389.
  • Manke, A., Wang, L., Rojanasakul, Y., 2013. Mechanisms of Nanoparticle-Induced Oxidative Stress and Toxicity. BioMed Research International 2013, ID 942916, 15 pages.
  • Miura, N., Shinohara, Y., 2009. Cytotoxic effect and apoptosis induction by silver nanoparticles in HeLa cells. Biochemical and Biophysical Research Communications 390, 733-737.
  • Naghsh, N., Valian, S., Madani, H., 2005. Investigation of beta cells specific apoptosis on the plasma free fatty acids level. Iranian Journal of Biochemistry and Molecular Biology 1, 15-16.
  • Napierska, D., Rabolli, V., Thomassen, L.C., Dinsdale, D., Princen, C., Gonzalez, L., Poels, K.L.C., Kirsch-Volders, M., Lison, D., Martens, J.A., Hoet, P.H., 2012. Oxidative stress induced by pure and iron-doped amorphous silica nanoparticles in subtoxic conditions. Chemical Research in Toxicology 25, 828-837.
  • Park, E., Bae, E., Yi, J., Kim, Y., Choid, K., Leed, S.H., Yoond, J., Leed, B.C., Parka, K., 2010. Repeated-dose toxicity and inflammatory responses in mice by oral administration of silver nanoparticles. Environmental Toxicology and Pharmacology 30, 162-168.
  • Park, S.H., Im, J.H., Im, J.W., Chun, B.H., Kim, J.H., 1999. Absorption kinetics of Au and Agnanoparticles on functionalized glass surfaces. Microchemical Journal 63, 71-91.
  • Pilz, J., Meineke, I., Gleiter, C.H., 2000. Measurement of free and bound malondialdehyde in plasma by high- performance liquid chromatography as the 2, 4- dinitrophenylhydrazine Chromatography B: Biomedical Sciences 742, 315-325. derivative. Journal of
  • Rosenman, K.D., Moss, A., Argyria, K.S., 1972. Clinical implications of exposure to silver nitrate and silver oxide. Journal of Occupational and Environmental Medicine 1921, 430-435.
  • Shukla, R.K., Sharma, V., Pandey, A.K., Singh, S., Sultana, S., Dhawan, A, 2011. ROS-mediated genotoxicity induced by titanium dioxide nanoparticles in human epidermal cells. Toxicology in Vitro 25, 231-241.
  • Soroka, N.F., Kostiuk, V.A., Potapovich, A.I., 1992. The mechanism of the antioxidant action of D- penicillamine. Eksperimental'naia i Klinicheskaia Farmakologiia 55, 42-43.
  • Squitti, R., Rossini, P.M., Cassetta, E., Moffa, F., Pasqualetti, P., Cortesi, M., Colloca, A., Rossi, L., Finazzi-Agró, A ., 2002. D-penicillamine reduces serum oxidative stress in Alzheimer’s disease patients. European Journal of Clinical Investigation 32, 51-59.
  • Stebounova, L.V., Adamcakova-Dodd, A., Kim, J.S., Park, H., O’Shaughnessy, P.T., Grassian, V.H., Thorne, P.S., 2011. Nanosilver induces minimal lung toxicity or inflammation in a subacute murine inhalation model. Particle and Fibre Toxicology 8, 5.
  • Suttnar, J., Masova, L., Dyr, E., 2001. Influence of citrate and EDTA anticoagulants on plasma malondialdehyde concentrations estimated by high-performance liquid chromatography. Journal of Chromatography B 751, 193-197.
  • Suttnar, J., Cermak, J., Dyr, J.E., 1997. Solid-phase extraction in analysis. malondialdehyde Biochemistry 249, 20-23. Annals of Clinical
  • Tang, J., Xiong, L., Wang, S., Wang, J., Liu, L., Li, J., Yuan, F., Xi, T., 2009. Distribution, translocation and accumulation of silver nanoparticles in rats. Journal of Nanoscience and Nanotechnology 9, 4924-4932.
  • Turski, M.L., Thiele, D.J., 2009. New roles for copper metabolismin cell proliferation, signaling, and disease. Journal of Biological Chemistry 284, 717-721.
  • Weast, R.C., Spadaro, J.A., Becker, R.O., 1988. Handbook of Chemistry and Physics, 69th ed. Boca Raton, CRC Press Inc: Boca Raton, Florida, USA, pp. 127-128.

BALB/c Farelerinde Oluşan Oksidatif Stres Üzerine Gümüş Nanopartiküllerle İndüklenmiş Penisilaminin Koruyucu Etkisinin Değerlendirilmesi

Yıl 2015, Cilt: 41 Sayı: 2, 205 - 211, 01.07.2015

Öz

Gümüş nanopartiküllerinin olumsuz etkilerine rağmen uygulamaları hızla artmaktadır. Bu çalışma nanogümüş uygulamasının histopatolojik değişiklikler ve biyokimyasal parametreler üzerindeki istenmeyen etkilerini ve aynı zamanda penisilaminin terapötik etkinliğini araştırmak için düzenlenmiştir.  Altışarlı dört grup BALB/c faresine değişik dozlardaki nanogümüş intraperitoneal yoldan uygulandı ve LD50 hesaplandı. Penisilaminin terapötik etkinliğini araştırmak amacıyla 42 fare eşit yedi gruba ayrıldı. Her gruptaki hayvanlara LD50 nanogümüş ve eşzamanlı olarak ya da nanogümüş enjeksiyonundan 4 saat sonra 50 ya da 200 mg/kg penisilinamin  intraperitoneal yoldan uygulandı. Toplam protein, albumin, toplam globulin, kolesterol, trigliserit, aspartat aminotransferaz (ALT), alanin aminotransferaz (ALT), alkalin fosfataz (ALP), toplam antioksidan kapasite (TAC) ve malondialdehit (MDA) de dahil olmak üzere serum biyokimyasal ve histopatolojik analizler yürütüldü. LD50 1500 ppm’di. Sonuçlar nanogümüş alan farelerde AST ve ALT enzimleri ve MDA düzeylerinde artış gösterirken TAC düzeylerinde azalma oluştu. Bu farklılıklar özellikle nanogümüş alan fareler ile nanogümüş ve 200 mg/kg penisilamin alan farelerde istatistiksel olarak anlamlıydı. Ayrıca, 1500 ppm nanogümüş alan farelerin doku bölümlerinin histopatolojik incelenmesinde karaciğer ve böbrek hasarı olduğu görüldü. Öte yandan, nanogümüşe maruz kalmış farelere karşılık 200 mg/kg penisilamin alan farelerde biyokimyasal parametreler ve doku lezyonlarında iyileşme oldu ve oksidatif stres boyutunun azaldığı belirlendi.

Kaynakça

  • Akradi, L., Sohrabi-Haghdoost, I., Djeddi, A., Mortazavi, P., 2012. Histopathologic apoptotic effect of nanosilver in liver of broiler chickens. African Journal of Biotechnology 11, 6207-6211.
  • Beattie, A.D., 1977. The use of D-penicillamine for lead poisoning. Proceedings of the Royal Society of Medicine 70(Suppl 3), 43-45.
  • Burtis, C.A., Ashwood, E.R., 1994. Textbook of Clinical Chemistry, 2nd edn. Saunders, Philadelphia, pp 735- 888.
  • Buzea, C., Pacheco, I.I., Robbie, K., 2007. Nanomaterials and nanoparticles: sources and toxicity. Biointerphases 2(4), MR17-MR71.
  • Choi, J.E., Kim, S., Ahn, J.H., Youn, P., Kang, J.S., Park, K., Yi, J., 2010. Induction of oxidative stress and apoptosis by silver nanoparticles in the liver of adult zebrafish. Aquatic Toxicology 100, 151-159.
  • Choi, S.C., 1990. Interval estimation of the LD50 based on an up-and-down experiment. Biometrics 46(2), 485-92.
  • Hsin, Y.H., Chen, C.F., Huang, S., Shih, T.S., Lai, P.S., Chueh, P.J., 2008. The apoptotic effect of nanosilveris mediated by a ROS- and JNK- dependent mechanism involving the mitochondrial pathway in NIH3T3 cells. Toxicology Letters 179, 130-139.
  • Hussain, S.M., Javorina, A.K., Schrand, A.M., Duhart, H.M., Ali, S.F., Schlager, J.J., 2006. The interaction of manganese nano-particles with PC-12 vells induces dopamine depletion. Toxicological Sciences 92, 456-463.
  • Ji , J.H., Jung, J.H., Kim, S.S., Yoon, J.U., Park, J.D., Choi, B.S., 2007. Twenty-eight-day inhalation toxicity study of silver nanoparticles in Sprague-Dawley rats. Inhalation Toxicology 19, 857-871.
  • Kim, S., Choi, J.E., Choi, J., Chung, K.H., Park, K., Yi, J., Ryu, D.Y., 2009. Oxidative stress-dependent toxicity of silver nanoparticles in human hepatoma cells. Toxicology in Vitro 23, 1076-1084.
  • Kim, W.Y., Kim, J., Park, J.D., Ryu, H.Y., Yu, I.J., 2009. of Histological accumulation of silver nanoparticles in kidneys of Fischer 344 rats. Journal of Toxicology and Environmental Health A 72, 1279-1284. in
  • Klaine, S.J., Alvarez, P.J., Batley, G.E., Fernandes, F., Handy, R.D., Lyon, D.Y., Mahendra, S., Mclaughlin, M.J., Lead, J.R., 2008. Nanomaterials in the environment: behavior, Environmental Toxicology Chemistry 27, 1825-1851. and effects.
  • Luoma, P.V., 2008. Cytochrome P450 and gene activation-from pharmacology to regression of atherosclerosis. European Journal of Clinical Pharmacology 64, 841-850. elimination and
  • Lykkesfeldt, J., 2001. Determination of malondialdehyde as dithiobarbituric acid adduct in biological samples by HPLC with fluorescence detection: comparison with ultraviolet-visible Chemistry 47, 1725-1727. Clinical
  • Machiedo, G.W., Powell, R.J., Rush, Jr.B.F., Swislocki, N.I., Dikdan, G., 1989. The incidence of decreased red blood cell deformability in sepsis and the association with oxygen free radical damage and multiple system organ failure. Archives of Surgery 124, 1386-1389.
  • Manke, A., Wang, L., Rojanasakul, Y., 2013. Mechanisms of Nanoparticle-Induced Oxidative Stress and Toxicity. BioMed Research International 2013, ID 942916, 15 pages.
  • Miura, N., Shinohara, Y., 2009. Cytotoxic effect and apoptosis induction by silver nanoparticles in HeLa cells. Biochemical and Biophysical Research Communications 390, 733-737.
  • Naghsh, N., Valian, S., Madani, H., 2005. Investigation of beta cells specific apoptosis on the plasma free fatty acids level. Iranian Journal of Biochemistry and Molecular Biology 1, 15-16.
  • Napierska, D., Rabolli, V., Thomassen, L.C., Dinsdale, D., Princen, C., Gonzalez, L., Poels, K.L.C., Kirsch-Volders, M., Lison, D., Martens, J.A., Hoet, P.H., 2012. Oxidative stress induced by pure and iron-doped amorphous silica nanoparticles in subtoxic conditions. Chemical Research in Toxicology 25, 828-837.
  • Park, E., Bae, E., Yi, J., Kim, Y., Choid, K., Leed, S.H., Yoond, J., Leed, B.C., Parka, K., 2010. Repeated-dose toxicity and inflammatory responses in mice by oral administration of silver nanoparticles. Environmental Toxicology and Pharmacology 30, 162-168.
  • Park, S.H., Im, J.H., Im, J.W., Chun, B.H., Kim, J.H., 1999. Absorption kinetics of Au and Agnanoparticles on functionalized glass surfaces. Microchemical Journal 63, 71-91.
  • Pilz, J., Meineke, I., Gleiter, C.H., 2000. Measurement of free and bound malondialdehyde in plasma by high- performance liquid chromatography as the 2, 4- dinitrophenylhydrazine Chromatography B: Biomedical Sciences 742, 315-325. derivative. Journal of
  • Rosenman, K.D., Moss, A., Argyria, K.S., 1972. Clinical implications of exposure to silver nitrate and silver oxide. Journal of Occupational and Environmental Medicine 1921, 430-435.
  • Shukla, R.K., Sharma, V., Pandey, A.K., Singh, S., Sultana, S., Dhawan, A, 2011. ROS-mediated genotoxicity induced by titanium dioxide nanoparticles in human epidermal cells. Toxicology in Vitro 25, 231-241.
  • Soroka, N.F., Kostiuk, V.A., Potapovich, A.I., 1992. The mechanism of the antioxidant action of D- penicillamine. Eksperimental'naia i Klinicheskaia Farmakologiia 55, 42-43.
  • Squitti, R., Rossini, P.M., Cassetta, E., Moffa, F., Pasqualetti, P., Cortesi, M., Colloca, A., Rossi, L., Finazzi-Agró, A ., 2002. D-penicillamine reduces serum oxidative stress in Alzheimer’s disease patients. European Journal of Clinical Investigation 32, 51-59.
  • Stebounova, L.V., Adamcakova-Dodd, A., Kim, J.S., Park, H., O’Shaughnessy, P.T., Grassian, V.H., Thorne, P.S., 2011. Nanosilver induces minimal lung toxicity or inflammation in a subacute murine inhalation model. Particle and Fibre Toxicology 8, 5.
  • Suttnar, J., Masova, L., Dyr, E., 2001. Influence of citrate and EDTA anticoagulants on plasma malondialdehyde concentrations estimated by high-performance liquid chromatography. Journal of Chromatography B 751, 193-197.
  • Suttnar, J., Cermak, J., Dyr, J.E., 1997. Solid-phase extraction in analysis. malondialdehyde Biochemistry 249, 20-23. Annals of Clinical
  • Tang, J., Xiong, L., Wang, S., Wang, J., Liu, L., Li, J., Yuan, F., Xi, T., 2009. Distribution, translocation and accumulation of silver nanoparticles in rats. Journal of Nanoscience and Nanotechnology 9, 4924-4932.
  • Turski, M.L., Thiele, D.J., 2009. New roles for copper metabolismin cell proliferation, signaling, and disease. Journal of Biological Chemistry 284, 717-721.
  • Weast, R.C., Spadaro, J.A., Becker, R.O., 1988. Handbook of Chemistry and Physics, 69th ed. Boca Raton, CRC Press Inc: Boca Raton, Florida, USA, pp. 127-128.
Toplam 32 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Araştırma Makalesi
Yazarlar

Fatemeh Namazı Bu kişi benim

Mehdi Fazelı Bu kişi benim

Yalda Zolghadrı Bu kişi benim

Saeed Mohammadınezhad Bu kişi benim

Saeed Nazıfı

Yayımlanma Tarihi 1 Temmuz 2015
Yayımlandığı Sayı Yıl 2015 Cilt: 41 Sayı: 2

Kaynak Göster

APA Namazı, F., Fazelı, M., Zolghadrı, Y., Mohammadınezhad, S., vd. (2015). Evaluation of Protective Effect of Penicillamine on Silver Nanoparticles-Induced Oxidative Stress in BALB/c Mice. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, 41(2), 205-211. https://doi.org/10.16988/iuvfd.2015.93581
AMA Namazı F, Fazelı M, Zolghadrı Y, Mohammadınezhad S, Nazıfı S. Evaluation of Protective Effect of Penicillamine on Silver Nanoparticles-Induced Oxidative Stress in BALB/c Mice. iuvfd. Ağustos 2015;41(2):205-211. doi:10.16988/iuvfd.2015.93581
Chicago Namazı, Fatemeh, Mehdi Fazelı, Yalda Zolghadrı, Saeed Mohammadınezhad, ve Saeed Nazıfı. “Evaluation of Protective Effect of Penicillamine on Silver Nanoparticles-Induced Oxidative Stress in BALB/C Mice”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 41, sy. 2 (Ağustos 2015): 205-11. https://doi.org/10.16988/iuvfd.2015.93581.
EndNote Namazı F, Fazelı M, Zolghadrı Y, Mohammadınezhad S, Nazıfı S (01 Ağustos 2015) Evaluation of Protective Effect of Penicillamine on Silver Nanoparticles-Induced Oxidative Stress in BALB/c Mice. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 41 2 205–211.
IEEE F. Namazı, M. Fazelı, Y. Zolghadrı, S. Mohammadınezhad, ve S. Nazıfı, “Evaluation of Protective Effect of Penicillamine on Silver Nanoparticles-Induced Oxidative Stress in BALB/c Mice”, iuvfd, c. 41, sy. 2, ss. 205–211, 2015, doi: 10.16988/iuvfd.2015.93581.
ISNAD Namazı, Fatemeh vd. “Evaluation of Protective Effect of Penicillamine on Silver Nanoparticles-Induced Oxidative Stress in BALB/C Mice”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 41/2 (Ağustos 2015), 205-211. https://doi.org/10.16988/iuvfd.2015.93581.
JAMA Namazı F, Fazelı M, Zolghadrı Y, Mohammadınezhad S, Nazıfı S. Evaluation of Protective Effect of Penicillamine on Silver Nanoparticles-Induced Oxidative Stress in BALB/c Mice. iuvfd. 2015;41:205–211.
MLA Namazı, Fatemeh vd. “Evaluation of Protective Effect of Penicillamine on Silver Nanoparticles-Induced Oxidative Stress in BALB/C Mice”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, c. 41, sy. 2, 2015, ss. 205-11, doi:10.16988/iuvfd.2015.93581.
Vancouver Namazı F, Fazelı M, Zolghadrı Y, Mohammadınezhad S, Nazıfı S. Evaluation of Protective Effect of Penicillamine on Silver Nanoparticles-Induced Oxidative Stress in BALB/c Mice. iuvfd. 2015;41(2):205-11.