Effect of Different Intrauterine Oxytetracycline Treatment on Reproductive Performance of Dairy Cows with Clinical Endometritis and Determination of Oxytetracycline Residues in Milk

Yıl 2016, Cilt: 42 Sayı: 1, 80 - 88, 11.08.2015

Meysam Makkı Hamid Gheısarı Mohammad Ahmadı

Öz

Main objective of this study was to determine whether intrauterine infusion of oxytetracycline (OTC) is an appropriate method for the treatment of postpartum endometritis in dairy cows or not. To this end, 280 lactating Holstein cows with clinical signs of endometritis were randomly assigned into three treatment groups and 186 cows were sampled for cytology experiment. In group 1 (n=73) cows were treated with intrauterine infusion of 5 g/cow 10% OTC. In group 2 (n=44), before intrauterine OTC treatment, caudal epidural anesthesia was used to eliminate straining. In group 3 (n=49) after intrauterine infusion of  5g/cow 10% OTC, 100 ml of normal saline was injected to reduce the concentration of OTC and 20 untreated cows were assigned into control group (group 4). Clinical treatment rates were 79.4%, 68.1%, 65.3 %and 50% in OTC, OTC and caudal epidural anesthesia (OTC+CEA), OTC+Serum and control groups, respectively (P>0.05). Cytological treatment rate in each experiment group was significantly higher than the cytological treatment rate in control group in the second examination (P<0.05). To find out OTC residues in milk by high performance liquid chromatography (HPLC) method, milk samples of 6 treated cows from each group were collected randomly 12, 24, 48, 72 and 96 hours after treatment. Maximum amounts of OTC residues were found 72 hours after treatment in all treatment groups. To evaluate the reproductive performance, the researchers compared 280 cows of treatment groups were compared with 1088 cows that were clinically healthy without any signs of clinical endometritis. The reproductive performance of the two groups were not significantly different, except days to first service which was lower in cows without clinical endometritis (P<0.05). According to the cytology study and the presence of antibiotic residues in milk samples at different times, the use of intrauterine OTC for treatment of clinical endometritis seems skeptical.

Anahtar Kelimeler

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Kaynakça

• Adams, H.R., 2001. Veterinary Pharmacology and Therapeutics, Iowa State University Press.
• Ahmadi, M., Nazifi, S., Ghaisari, H., 2005b. The effect of intrauterine cephapirin on treatment of endometritis in commercial dairy cattle. Archives of Razi 59, 35-45.
• Ahmadi, M., Tafti, A.K., Nazifi, S., Ghaisari, H., 2005a. The comparative evaluation of uterine and cervical mucosa cytology with endometrial histopathology in cows. Comparative Clinical Pathology 14, 90-94.
• Ahmadi, M.R., Hosseini, A., Gheisari, H.R., Yavari, M., 2014. Preliminary trial in treatment of postpartum endometritis with intrauterine application of hyperimmune serum in dairy cows. Asian Pacific Journal of Tropical Disease 4, S360-S365.
• Brick, T., Schuenemann, G., Bas, S., Daniels, J., Pinto, C., Rings, D., Rajala-Schultz, P., 2012. Effect of intrauterine dextrose or antibiotic therapy on reproductive performance of lactating dairy cows diagnosed with clinical endometritis. Journal of Dairy Science 95, 18941905.
• Cohen, R., Bernstein, M., Ziv, G., 1995. Isolation and antimicrobial susceptibility of Actinomyces pyogenes recovered from the uterus of dairy cows with retained fetal membranes and post parturient endometritis. Theriogenology 43, 1389-1397.
• Drillich, M., Raab, D., Wittke, M., Heuwieser, W., 2005. Treatment of chronic endometritis in dairy cows with an intrauterine application of enzymes: A field trial. Theriogenology 63, 1811-1823.
• Fritz, J.W., Zuo, Y., 2007. Simultaneous determination of tetracycline, oxytetracycline, and 4-epitetracycline in milk by high-performance liquid chromatography. Food Chemistry 105, 1297-1301.
• Kaale, E., Chambuso, M., Kitwala, J., 2008. Analysis of residual oxytetracycline in fresh milk using polymer reversedphase column. Food Chemistry 107, 1289-1293.
• Kaczmarowski, M., Malinowski, E., Markiewicz, H., 2003. Influence of various treatment methods on bacteriological findings in cows with puerperal endometritis. Polish Journal of Veterinary Sciences 7, 171-174.
• Kaneene, J., Coe, P.H., Smith, J.H., Rapnicki, P., Smith, C.L., Gerloff, B., Morrow, D.A., 1986. Drug residues in milk after i.u. injection of oxitetracycline, lincomycinspectinomycin and povidone iodide in cows with metritis. American Journal of Veterinary Research 47, 1363-1365.
• Kroger, M., 1985. Milk sample preservation. Journal of Dairy Science 68, 783-787.
• LeBlanc, S., Duffield, T., Leslie, K., Bateman, K., Keefe, G.P., Walton, J., Johnson, W., 2002. The effect of treatment of clinical endometritis on reproductive performance in dairy cows. Journal of Dairy Science 85, 2237-2249.
• LeBlanc, S.J., 2008. Postpartum uterine disease and dairy herd reproductive performance: A review. The Veterinary Journal 176, 102-114.
• Lu, W., Zabuli, J., Kuroiwa, T., Tanaka, T., Kamomae, H., 2011. Effect of intrauterine infusion with liquid paraffin on phagocytes migrating to mucus of external os of the cervix in cows. Reproduction in Domestic Animals 46, 602-607.
• Naoto, F., 1999. Rapid liquid chromatographic determination of oxyetracycline in milk. Journal of Chromatography A839, 247-251.
• Navratilova, P., Borkovcova, I., Dračková, M., Janštová, B., Vorlova, L., 2009. Occurrence of tetracycline, chlortetracyclin, and oxytetracycline residues in raw cow's milk. Czech Journal of Food Sciences 27, 379385.
• Noakes, P.T., England G.C.W., 2009. Veterinary Reproduction and Obstetrics, Saunders Elsevier, pp. 411-417.
• Overton, M., Fetrow, J., 2008. Economics of postpartum uterine health. Proceedings of the 2008 dairy cattle reproduction council convention, pp. 39-43, Omaha, Nebraska.
• Papadoyannis, I., Samanidou, V., Kovatsi, L., 2000. A rapid high performance liquid chromatographic (HPLC) assay for the determination of oxytetracycline in commercial pharmaceuticals. Journal of Pharmaceutical and Biomedical Analysis 23, 275-280.
• Parasad, S., Jayachandran, C., Singh, M., 1987. Pharmacokinetics of oxytetracycline in dairy cows. Indian Journal of Animal Sciences 57, 106-110.
• Roncada, P., Ermini, L., Schleuning, A., Stracciari, G., Strocchia, A., 2000. Pharmacokinetics and residual behaviour in milk of oxytetracycline in cows following administration of uterine pessaries. Journal of Veterinary Pharmacology and Therapeutics 23, 281285.
• Samanidou, V.F., Nikolaidou, K.I., Papadoyannis, I.N., 2007. Development and validation of an HPLC confirmatory method for the determination of seven tetracycline antibiotics residues in milk according to the European Union Decision 2002/657/EC. Journal of Separation Science 30, 2430-2439.
• Sheldon, I., 2007. Endometritis in cattle: Pathogenesis, consequences for fertility, diagnosis and therapeutic recommendations. Partners in Reproduction 2, 1-5.
• Sheldon, I., Noakes, D., Rycroft, A., Dobson, H., 2004. Effect of intrauterine administration of oestradiol on postpartum uterine bacterial infection in cattle. Animal Reproduction Science 81, 13-23.
• Sheldon, I.M., Lewis, G.S., LeBlanc, S., Gilbert, R.O., 2006. Defining postpartum uterine disease in cattle. Theriogenology 65, 1516-1530.
• Sheldon, I.M., Williams, E.J., Miller, A.N., Nash, D.M., Herath, S., 2008. Uterine diseases in cattle after parturition. The Veterinary Journal 176, 115-121.
• Sokol, J., Matisova, E., 1994. Determination of tetracycline antibiotics in animal tissues of food-producing animals by high-performance liquid chromatography using solid-phase extraction. Journal of Chromatography A 669, 75-80.
• Swelum, A.A.A., 2013. Clinical endometritis in the camel (Camelus dromedarius): A field study. Journal of Animal and Veterinary Advances 12, 527-532.
• Tan, X., Huang, Y. J., Jiang, Y.W., Hu, S.H., 2007. Persistence of oxytetracycline residues in milk after the intrauterine treatment of lactating cows for endometritis. Veterinary Record 161(17), 585-586.
• Tranquilli, W.J., Thurmon, J.C., Grimm, K.A., 2013. Lumb and Jones' Veterinary Anesthesia and Analgesia, John Wiley & Sons.
• Vesal, N., Ahmadi, M., Foroud, M., Imani, H., 2013. Caudal epidural anti‐nociception using lidocaine, bupivacaine or their combination in cows undergoing reproductive procedures. Veterinary Anaesthesia and Analgesia 40, 328-332.
• Vuković, V., Kasalica, A., Vićentijević, M., 2011. Examination of middle concentrations of oxytetracycline residues in cow milk after intrauterine application. Biotechnology in Animal Husbandry 27, 929-934.
• Williams, E.J., Fischer, D.P., Pfeiffer, D.U., England, G.C., Noakes, D.E., Dobson, H., Sheldon, I.M., 2005. Clinical evaluation of postpartum vaginal mucus reflects uterine bacterial infection and the immune response in cattle. Theriogenology 63, 102-117.