Short Period Starvation in Rat: The Effect of Aloe Vera Gel Extract on Oxidative Stress Status Ion

Yıl 2017, Cilt: 43 Sayı: 1, 32 - 38, 01.01.2017

Laleh Shahrakı Mojahed Mehdi Saeb Mohammad Mohsen Mohammadı Saeed Nazıfı

https://doi.org/10.16988/iuvfd.270101

Öz

    The main aim of this experiment was to evaluate the effect of Aloe vera gel extract on oxidative stress status during starvation. For this purpose, twenty-four mature male albino Wistar rats were housed in standard cages. In this study starvation cycle (rats were starved for two days and then were fed for one day) was used. This study was performed during short period (20 days).Animals were divided into four experimental groups (six rats in each group): 1) normal control; 2) starved rats+water/ethanol; 3) starved rats+hydro-alcoholic Aloe vera gel extract (100 mg/kg); 4) starved rats+hydro-alcoholic Aloe vera gel extract (200 mg/kg). Blood samples were obtained using cardiac puncture. In blood samples, antioxidant enzymes including superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT), antioxidant trace elements including copper, zinc and manganese and antioxidant vitamins including vitamin A, vitamin E and vitamin C were measured. Plasma levels of antioxidant enzymes (SOD, GPx and catalase) significantly decreased in starved rats+water/ethanol group (P<0.05). Plasma levels of antioxidant enzymes after treatment with hydro-alcoholic Aloe vera gel extract at doses 100 and 200 mg/kg were significantly increased (P<0.05). Plasma levels of Cu, Zn and Mn in normal control group had no significant difference with starved rats+water/ethanol and starved rats+hydro-alcoholic Aloe vera gel extract in 100 and 200 mg/kg dose groups (P>0.05). Plasma levels of vitamin A and E in normal control group had no significant difference with starved rats+water/ethanol and starved rats+hydro-alcoholic Aloe vera gel extract in the 100 and 200 mg/kg dose groups (P>0.05). Plasma level of vitamin C significantly decreased in starved rats+water/ethanol group (P<0.05). Plasma level of vitamin C after treatment with hydro-alcoholic Aloe vera gel extract at doses 100 and 200 mg/kg were significantly increased (P<0.05). Our results shown that short term starvation caused an increase in oxidative stress via impairing of antioxidant defense and Aloe vera treatment is able to improve antioxidative defense induced by starvation.

Anahtar Kelimeler

Aloe vera gel extract, oxidative stress, rat, starvation

Kaynakça

• Asayama, K., Hayashibe, H., Dobashi, K., Niitsu, T., Miyao, A., Kato, K., 1989. Antioxidant enzyme status and lipid peroxidation in various tissues of diabetic and starved rats. Diabetes Research 12, 85-91.
• Berggren Söderlund, M., Fex, G., Nilsson‐Ehle, P., 2003. Decreasing serum concentrations of all‐trans, 13‐cis retinoic acids and retinol during fasting and caloric restriction. Journal of Internal Medicine 253, 375-380.
• Cock, I.E., Sirdaarta, J., 2011. The toxicity of Aloe barbadensis Miller juice is due to the induction of oxidative stress. Advances in Environmental Biology 288-300.
• Crescimanno, M., Armata, M.G., Rausa, L., Gueli, M.C., Nicotra, C., D'alessandro, N., 1989. Cardiac peroxisomal enzymes and starvation. Free Radical Research Communications 7, 67-72.
• Domenicali, M., Caraceni, P., Vendemiale, G., Grattagliano, I., Nardo, B., Dall’Agata, M., Santoni, B., Trevisani, F., Cavallari, A., Altomare, E., Bernardi, M., 2001. Food deprivation exacerbates mitochondrial oxidative stress in rat liver exposed to ischemia-reperfusion injury. The Journal of Nutrition 131, 105-110.
• Fukuwatari, T., Yoshida, E., Takahashi, K., Shibata, K., 2010. Effect of fasting on the urinary excretion of water-soluble vitamins in humans and rats. Journal of Nutritional Science and Vitaminology 56, 19-26.
• Geiger, S., Kauffmann, M., Le Maho, Y., Robin, J.P., Criscuolo, F., 2012. Of the importance of metabolic phases in the understanding of oxidative stress in prolonged fasting and refeeding. Physiological and Biochemical Zoology 85, 415-420.
• Gîlcă, M., Soian, I., Mohora, M., Petec, C., Muscurel, C., Dinu, V., 2002. The effect of fasting on the parameters of the antioxidant defence system in the blood of vegetarian human subjects. Romanian Journal of Internal Medicine 41, 283-292.
• Godin, D.V., Wohaieb, S.A., 1988. Nutritional deficiency, starvation, and tissue antioxidant status. Free Radical Biology and Medicine 5, 165-176.
• Haritha, K., Ramesh, B., Saralakumari, D., 2014. Effect of Aloe vera gel on antioxidant enzymes in streptozotocin-induced cataractogenesis in male and female Wistar rats. Journal of Acute Medicine 4, 38-44.
• Hupert, J., Cunningham, K.N., Mobarhan, S., Friedman, H.J., Layden, T.J., 1989. Effects of acute starvation on vitamin A status in rats. Journal of the American College of Nutrition 8, 644-649.
• Jain, N.C., 1986. Schalm’s Veterinary Hematology. 4th Edition. Lea and Febiger.
• Johnson-Davis, K.L., Moore, S.J., Owen, W.E., Cutler, J.M., Frank, E.L., 2009. A rapid HPLC method used to establish pediatric reference intervals for vitamins A and E. Clinica Chimica Acta 405, 35-38.
• Kono, Y., Fridovich, I., 1982. Superoxide radical inhibits catalase. Journal of Biological Chemistry 257, 5751-5754.
• Limón-Pacheco, J., Gonsebatt, M.E., 2009. The role of antioxidants and antioxidant-related enzymes in protective responses to environmentally induced oxidative stress. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 674, 137-147.
• Malik, I., Zarnigar, H.N., 2013. Aloe vera-A Review of its Clinical Effectiveness. International Research Journal of Pharmacy 4, 75-79.
• Marczuk-Krynicka, D., Hryniewiecki, T., Piątek, J., Paluszak, J., 2003. The effect of brief food withdrawal on the level of free radicals and other parameters of oxidative status in the liver. Medical Science Monitor Basic Research 9, BR131-BR135.
• Mohamed, E.A.K., 2011. Antidiabetic, antihypercholestermic and antioxidative effect of Aloe vera gel extract in alloxan induced diabetic rats. Australian Journal of Basic and Applied Sciences 5, 1321-1327.
• Nahar, T., Uddin, B., Hossain, S., Sikder, A.M., Ahmed, S., 2013. Aloe vera gel protects liver from oxidative stress-induced damage in experimental rat model. Journal of Complementary and Integrative Medicine 10, 47-53.
• Orellana, M., Fuentes, O., Rosenbluth, H., Lara, M., Valdés, E., 1992. Modulation of rat liver peroxisomal and microsomal fatty acid oxidation by starvation. FEBS Letters 310, 193-196.
• Paglia, D.E., Valentine, W.N., 1967. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. The Journal of Laboratory and Clinical Medicine 70, 158-169.
• Puls, R., 1994. Mineral levels in animal health. Diagnostic data. Sherpa International. British Columbia, Canada.
• Radha, M.H., Laxmipriya, N.P., 2015. Evaluation of biological properties and clinical effectiveness of Aloe vera: A systematic review. Journal of Traditional and Complementary Medicine 5, 21-26.
• Ramachandraiahgari, R.M.Y., Somesula, S.R., Adi, P.J., Mannur, I.S., Enamala, M., Matcha, B., 2012. Protective role of ethanolic extract of Aloe vera antioxidant properties on liver and kidney of streptozotocin-induced diabetic rats. Digest Journal of Nanomaterials and Biostructures 7, 175-184.
• Robinson, M.K., Rustum, R.R., Chambers, E.A., Rounds, J.D., Wilmore, D.W., Jacobs, D.O., 1997. Starvation enhances hepatic free radical release following endotoxemia. Journal of Surgical Research 69, 325-330.
• Slaughter, M.R., O’Brien, P.J., 2000. Fully-automated spectrophotometric method for measurement of antioxidant activity of catalase. Clinical Biochemistry 33, 525-534.
• Sorensen, M., Sanz, A., Gomez, J., Pamplona, R., Portero-Otin, M., Gredilla, R., Barja, G., 2006. Effects of fasting on oxidative stress in rat liver mitochondria. Free Radical Research 40, 339-347.
• Stavric, B., 1994. Role of chemopreventers in human diet. Clinical Biochemistry 27, 319-332.
• Stirpe, F., Comporti, M., 1965. Regulation of ascorbic acid and of xylulose synthesis in rat-liver extracts. The effect of starvation on the enzymes of the glucuronic acid pathway. Biochemical Journal 95, 354-362.
• Trivedi, N., Rishi, P., Soni, S.K., 2015. Protective role of aloe vera wine against oxidative stress induced by Salmonella infection in a murine model. International Journal of Food and Nutritional Sciences 4, 64-76.
• Valko, M., Leibfritz, D., Moncol, J., Cronin, M.T.D., Mazur, M., Telser, J., 2007. Free radicals and antioxidants in normal physiological functions and human disease. The International Journal of Biochemistry and Cell Biology 39, 44-84.
• Wasselin, T., Zahn, S., Maho, Y.L., Dorsselaer, A.V., Raclot, T., Bertile, F., 2014. Exacerbated oxidative stress in the fasting liver according to fuel partitioning. Proteomics 14, 1905-1921.