TEKRARLAYAN GEBELİK KAYIPLARINDA SİTOGENETİK ANALİZ

Öz

Retrospektif olarak gerçekleştirdiğimiz çalışmamızda, örnek grubumuz 2008-2012 Şifa Üniversitesi Tıp Fakültesi Genetik Tanı Merkezi’ne Kadın Hastalıkları ve Doğum Kliniği’nden tekrarlayan gebelik kaybı nedeniyle gönderilen düşük materyallerinden oluşmuştur. 96 adet düşük materyalinden uzun süreli hücre kültürü yapılmıştır. 84 örnekte hücre kültürü başarılı, 12 örnekte başarılı olmamıştır. 84 örneğin sitogenetik analizi sonucunda örneklerin 56’sında %67 normal kromozom kuruluşu, 24’ünde %29 anormal kromozom kuruluşu, 2‘sinde %2 mozaik kromozom kuruluşu ve 2 ‘sinde %2 polimorfizm saptanmıştır. Anormal kromozom kuruluşunun % 96’sında sayısal anomali, %4’ünde yapısal anomali saptanmıştır. Sayısal anomaliler içinde %82.5 anöploidi ve anöploidiler içinde en sık trizomi 16 ‘ya %16 rastlanılmıştır. Normal karyotip saptanan olgularda %84 oranında 46,XX kromozom kuruluşu, %16 oranında 46,XY kromozom kuruluşu saptanmıştır

Anahtar Kelimeler

• Tekrarlayan gebelik kaybı,sitogenetik analiz

CYTOGENETIC ANALYSIS IN RECURRENT PREGNANCY LOSS

Öz

Our study was done retrospective, sample group has consisted abort materials that have received a diagnosis of recurrent pregnancy loss sent from Şifa University Faculty of Medicine, Department of Obstetrics and Gynecology between 2008 and 2012. The long term cell culture has been done on 96 abort materials. The cell culture has became succesful in 84 materials, culture has became failure in 12 materials. The result of cytogenetic analysis on 84 materials has been presented that in 56 of them 67% normal karyotype, in 24 29% anormal karyotype, in 2 2% mosaic karyotype and in 2 2% polymorphism. It has been determined 96% numerical abnormality, 4% structural abnormality. It has been observed 82.5% aneuploidy in numerical abnormalities and so most common trisomy 16 16% in aneuploides. In the cases where normal kayotype havebeen determined 84% of them are 46,XX karyotype and 16% of them are 46,XY karyotype

Anahtar Kelimeler

• Recurrent pregnancy loss,cytogenetic analysis

Kaynakça

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