Bitki Fungal Hastalıkları ile Biyolojik Savaşımda Yeni Yaklaşımlar: Fungal Virüsler

Yıl 2019, Cilt: 2 Sayı: 1, 33 - 48, 30.04.2019

Çiğdem Özkan Kahraman Figen Yıldız

Öz

Fungal
virüsler diğer adıyla mikovirüsler bitki patojeni funguslarda yaygın olarak
bulunan obligat parazitlerdir. Mikovirüslerin çoğunluğu dsRNA genomuna sahip
olmakla birlikte, DNA genomuna sahip mikovirüsler ile ilgili çalışmalar da
bulunmaktadır. Mikovirüsler, fungal hücre içerisinde hücre birleşmesi
(anastomosis), hücre bölünmesi veya eşeyli-eşeysiz fungal yapılar sayesinde
yayılış göstermektedir. Enfeksiyon için hücre dışı taşınmadan yoksun olan
mikovirüsler konukçusunda fenotipik değişikliklere neden olmakta, virulensliği
arttırmakta ya da azaltmaktadır. Özellikle virulensliği azaltma (hipovirulenslik)
etkisi nedeniyle, mikovirüsleri biyolojik mücadelede kullanmak mümkün
olabilmektedir. Ülkemizde ve dünyada yapılan çalışmalarda bazı önemli fungal
patojenlerde pek çok mikovirüs saptanmış ve biyolojik mücadelede
kullanılmıştır. Yapılacak çalışmalarda mikovirüslerin yapısal analizleri,
virüsün fonksiyonunun ve evriminin daha iyi anlaşılmasını sağlayacaktır.
Mikovirüsler ve bitki patojeni funguslar arasındaki etkileşimi anlamak, onların
bitki hastalıklarında biyolojik mücadelede kullanım olanaklarını
geliştirebilmek için kaçınılmazdır.

Anahtar Kelimeler

Mikovirüs, dsRNA, hipovirulenslik

Kaynakça

• Akıllı, S., Ulubaş-Serçe, Ç., Katırcıoğlu, Y.Z., Maden, S., Rigling, D. (2012). Characterization of hypovirulent isolates of the chestnut blight fungus, Cryphonectria parasitica from the Marmara and Black Sea regions of Turkey. European Journal of Plant Pathology, 135(2),323-334.Allemann, C., Hoegger, P., Heiniger, U., Rigling, D. (1999). Genetic variation of Cryphonectria hypoviruses (CHV1) in Europe, assessed using restriction fragment length polymorphism (RFLP) markers. Molecular Ecology, 8(5), 843-854.Cai, G., Hillman, B. I. (2013). Phytophthora viruses. Advances in virus research, 86,327-350.Cañizares, M. C., Pérez-Artés, E., García-Pedrajas, M. D. (2014). The complete nucleotide sequence of a novel partitivirus isolated from the plant pathogenic fungus Verticillium alboatrum. Archives of Virology, 159, 3141–3144.Cao, Y.-F., Zhu, X.-W., Xiang, Y., Li, D.-Q., Yang, J.-R., Mao, Q.-Z., Chen, J. S. (2011). Genomic characterization of a novel dsRNA virus detected in the phytopathogenic fungus Verticillium dahliae Kleb. Virus Research, 159,73–78. Castro, M., Kramer, K., Valdivia, L., Ortiz, S., Castillo, A. (2003). A double-stranded RNA mycovirus confers hypovirulence-associated traits to Botrytis cinerea. FEMS Microbiology Letters, 228(1),87-91.Chen, B., Nuss, D. L. (1999). Infectious cDNA clone of hypovirus CHV1-Euro7: a comparative virology approach to investigate virus-mediated hypovirulence of the chestnut blight fungus Cryphonectria parasitica. Journal of Virology, 73(2), 985-992.Cho, W. K., Lee, K. M., Yu, J., Son, M., Kim, K. H. (2013). Insight into mycoviruses infecting Fusarium species. Advances in Virus Research, 86, 273-288.Cho, W. K., Yu, J., Lee, K. M., Son, M., Min, K., Lee, Y. W., Kim, K. H. (2012). Genome-wide expression profiling shows transcriptional reprogramming in Fusarium graminearum by Fusarium graminearum virus 1-DK21 infection. Bmc Genomics, 13(1),173.Choi, G. H., Nuss, D. L. (1992). Hypovirulence of chestnut blight fungus conferred by an infectious viral cDNA. Science, 257(5071),800-803.Chun, S. J., Lee, Y. H. (1997). Inheritance of dsRNAs in the rice blast fungus, Magnaporthe grisea. FEMS microbiology letters, 148(2),159-162.Darissa, O., Adam, G., Schäfer, W. (2012). A dsRNA mycovirus causes hypovirulence of Fusarium graminearum to wheat and maize. European Journal of Plant Pathology, 134(1),181-189.Deng, F., Allen, T. D., Hillman, B. I., Nuss, D. L. (2007). Comparative analysis of alterations in host phenotype and transcript accumulation following hypovirus and mycoreovirus infections of the chestnut blight fungus Cryphonectria parasitica. Eukaryotic Cell, 6(8), 1286-1298.Ghabrial, S. A., Dunn, S. E., Li, H., Xie, J., Baker, T. S. (2013). Viruses of Helminthosporium (Cochlioblus) victoriae. Advances in Virus Research, 86, 289-325.Ghabrial, S. A., Castón, J. R., Jiang, D., Nibert, M. L., Suzuki, N. (2015). 50-plus years of fungalviruses. Virology, 479–480, 356–368.Göker, M., Scheuner, C., Klenk, H. P., Stielow, J. B., Menzel, W. (2011). Codivergence of mycoviruses with their hosts. PLoS One, 6(7), e22252.Griffin, G. J., Robbins, N., Hogan, E. P., Farias‐Santopietro, G. (2004). Nucleotide sequence identification of Cryphonectria hypovirus 1 infecting Cryphonectria parasitica on grafted American chestnut trees 12–18 years after inoculation with a hypovirulent strain mixture. Forest Pathology, 34(1), 33-46.Hillman, B.I., Suzuki, N. (2004). Viruses of the chestnut blight fungus, Cryphonectria parasitica. Adv. Virus Res, 63, 423-472.Hollings, M. (1962). Viruses associated with a die-back disease of cultivated mushroom. Nature, 196(4858), 962.Howitt, R. L., Beever, R. E., Pearson, M. N., Forster, R. L. (1995). Presence of double-stranded RNA and virus-like particles in Botrytis cinerea. Mycological Research, 99(12),1472-1478.ICTV (2014). International committee on taxonomy of viruses. Erişim adresi: http://ictvonline.org/index.asp Erişim tarihi: 10.12.2018Ikeda, K., Inoue, K., Kida, C., Uwamori, T., Sasaki, A., Kanematsu, S., Park, P. (2013). Potentiation of mycovirus transmission by zinc compounds via attenuation of heterogenic incompatibility in Rosellinia necatrix. Applied and Environmental Microbiology, 79(12), 3684-3691.Jiang, D., Ghabrial, S. A. (2004). Molecular characterization of Penicillium chrysogenum virus: reconsideration of the taxonomy of the genus Chrysovirus. Journal of General Virology, 85(7),2111-2121.Khalifa, M. E., Pearson, M. N. (2013). Molecular characterization of three mitoviruses co-infecting a hypovirulent isolate of Sclerotinia sclerotiorum fungus. Virology, 441(1), 22-30.Kwon, S. J., Cho, S. Y., Lee, K. M., Yu, J., Son, M., Kim, K. H. (2009). Proteomic analysis of fungal host factors differentially expressed by Fusarium graminearum infected with Fusarium graminearum virus-DK21. Virus Research, 144(1-2),96-106.Lampson, G. P., Tytell, A. A., Field, A. K., Nemes, M. M., Hilleman, M. R. (1967). Inducers of interferon and host resistance. I. Double-stranded RNA from extracts of Penicillium funiculosum. Proceedings of the National Academy of Sciences, 58(2),782-789.Li, H., Fu, Y., Jiang, D., Li, G., Ghabrial, S. A., Yi, X. (2008). Down-regulation of Sclerotinia sclerotiorum gene expression in response to infection with Sclerotinia sclerotiorum debilitation-associated RNA virus. Virus Research, 135(1), 95-106.Marvelli, R. A., Hobbs, H. A., Li, S., McCoppin, N. K., Domier, L. L., Hartman, G. L., Eastburn, D. M. (2014). Identification of novel double-stranded RNA mycoviruses of Fusarium virguliforme and evidence of their effects on virulence. Archives of Virology, 159(2), 349-352.Mersin, E., Açıkgöz, S., Erincik, Ö. (2018). Cryphonectria parasitica’nın hipovirülent strainlerinin fenol ve kloroform içermeyen dsRNA analiz yöntemi ile belirlenmesi ve Cryphonectria hypovirus 1’in RT-PCR İle Tanılanması. Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi, 15(2), 25-32.Muñoz-Adalia, E. J., Fernández, M. M., Diez, J. J. (2016). The use of mycoviruses in the control of forest diseases. Biocontrol Science and Technology, 26(5), 577-604.Nuss, D.L. (2011). Mycoviruses, RNA silencing, and viral RNA recombination. Adv. Virus Res, 80, 25-48.Pearson, M. N., Beever, R. E., Boine, B., Arthur, K. (2009). Mycoviruses of filamentous fungi and their relevance to plant pathology. Molecular Plant Pathology, 10(1),115-128.Peever, T. L., Liu, Y. C., Cortesi, P., Milgroom, M. G. (2000). Variation in tolerance and virulence in the chestnut blight fungus-hypovirus interaction. Applied and Environmental Microbiology, 66(11), 4863-4869.Rogers, H. J., Buck, K. W., Brasier, C. M. (1987). A mitochondrial target for double-stranded RNA in diseased isolates of the fungus that causes Dutch elm disease. Nature, 329(6139), 558.Shapira, R., Choi, G. H., Nuss, D. L. (1991). Virus‐like genetic organization and expression strategy for a double‐stranded RNA genetic element associated with biological control of chestnut blight. The EMBO Journal, 10(4),731-739.Smart, C. D., Yuan, W., Foglia, R., Nuss, D. L., Fulbright, D. W., Hillman, B. I. (1999). Cryphonectria hypovirus 3, a virus species in the family Hypoviridae with a single open reading frame. Virology, 265(1), 66-73.Sutherland, M. L., Brasier, C. M. (1995). Effect of d-factors on in vitro cerato-ulmin production by the Dutch elm disease pathogen Ophiostoma novo-ulmi. Mycological Research, 99(10),1211-1217.Wang, L., Jiang, J., Wang, Y., Hong, N., Zhang, F., Xu, W., Wang, G. (2014). Hypovirulence of the phytopathogenic fungus Botryosphaeria dothidea: association with a co-infecting chrysovirus and a partitivirus. Journal of Virology, JVI-00538.Wang, S., Kondo, H., Liu, L., Guo, L., Qiu, D. (2013). A novel virus in the family Hypoviridae from the plant pathogenic fungus Fusarium graminearum. Virus Research, 174(1-2), 69-77.Wang, S., Ongena, M., Qiu, D., Guo, L. (2017). Fungal viruses: Promising fundamental research and biological control agents of fungi. SM Virol, 2(1), 1011.Wu, M., Jin, F., Zhang, J., Yang, L., Jiang, D., Li, G. (2012). Characterization of a novel bipartite double-stranded RNA mycovirus conferring hypovirulence in the phytopathogenic fungus Botrytis porri. Journal of Virology, JVI-00292.Xie, J., Ghabrial, S. A. (2012). Molecular characterizations of two mitoviruses co-infecting a hyovirulent isolate of the plant pathogenic fungus Sclerotinia sclerotiorum. Virology, 428(2), 77-85.Xie, J., Jiang, D. (2014). New insights into mycoviruses and exploration for the biological control of crop fungal diseases. Annual Review of Phytopathology, 52, 45-68.Xie, J., Xiao, X., Fu, Y., Liu, H., Cheng, J., Ghabrial, S. A., Li, G., Jiang, D. (2011). A novel mycovirus closely related to hypoviruses that infects the plant pathogenic fungus Sclerotinia sclerotiorum. Virology, 418(1), 49-56.Yaegashi, H., Nakamura, H., Sawahata, T., Sasaki, A., Iwanami, Y., Ito, T., Kanematsu, S. (2012). Appearance of mycovirus-like double-stranded RNAs in the white root rot fungus, Rosellinia necatrix, in an apple orchard. FEMS Microbiology Ecology, 83(1),49-62.Yaegashi, H., Yoshikawa, N., Ito, T., Kanematsu, S. (2013). A mycoreovirus suppresses RNA silencing in the white root rot fungus, Rosellinia necatrix. Virology, 444(1-2), 409-416.Yu, X., Li, B., Fu, Y., Jiang, D., Ghabrial, S. A., Li, G., Peng, Y., Xie, J., Cheng, J., Huang, J., Yi, X. (2010). A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus. Proceedings of the National Academy of Sciences, 107(18), 8387-8392.Yu, X., Li, B., Fu, Y., Xie, J., Cheng, J., Ghabrial, S. A., Li, G., Yi, X., Jiang, D. (2013). Extracellular transmission of a DNA mycovirus and its use as a natural fungicide. Proceedings of the National Academy of Sciences, 110(4), 1452-1457. Zheng, L., Zhang, M., Chen, Q., Zhu, M., Zhou, E. (2014). A novel mycovirus closely related to viruses in the genus Alphapartitivirus confers hypovirulence in the phytopathogenic fungus Rhizoctonia solani. Virology, 456, 220-226.Zhu, W., Wei, W., Fu, Y., Cheng, J., Xie, J., Li, G., Yi, X., Kang, Z., Dickman, M. B., Jiang, D. (2013). A secretory protein of necrotrophic fungus Sclerotinia sclerotiorum that suppresses host resistance. PLoS One, 8(1), e53901.