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Yıl 2009, Cilt: 3 Sayı: 3, 71 - 75, 01.11.2009

Öz

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Partial characterization of proteases produced by three fungal isolates from the rhizosphere of wild yam Dioscorea wallichii

Yıl 2009, Cilt: 3 Sayı: 3, 71 - 75, 01.11.2009

Öz

Among the twenty different fungal members isolated from the rhizosphere of wild yam, Dioscorea wallichii, three fungal isolates were found to be the best protease producers and identified as Aspergillus sp., Penicillium sp. and Trichoderma sp. The protease production studies of these three isolates were conducted up to 12 days. After nine days there was no significant increase in the enzyme production. Maximum protease activity was observed in the range of pH 6 and temperature 30˚C. Protease from all the three isolates was stable up to 2 h in dettol, tween 20, tween 80 and soap solution. Among the different immobilization techniques used agar block was the most effective. The molecular weight, zymogram analysis and destaining activity of the protease enzyme were also studied

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Ayrıntılar

Diğer ID JA39KE57JC
Bölüm Araştırma Makalesi
Yazarlar

Nallusamy Sıvakumar Bu kişi benim

Rajendran Remya Bu kişi benim

Al Bahry Saıf Bu kişi benim

Yayımlanma Tarihi 1 Kasım 2009
Yayımlandığı Sayı Yıl 2009 Cilt: 3 Sayı: 3

Kaynak Göster

APA Sıvakumar, N., Remya, R., & Saıf, A. B. (2009). Partial characterization of proteases produced by three fungal isolates from the rhizosphere of wild yam Dioscorea wallichii. Journal of Applied Biological Sciences, 3(3), 71-75.
AMA Sıvakumar N, Remya R, Saıf AB. Partial characterization of proteases produced by three fungal isolates from the rhizosphere of wild yam Dioscorea wallichii. J.appl.biol.sci. Kasım 2009;3(3):71-75.
Chicago Sıvakumar, Nallusamy, Rajendran Remya, ve Al Bahry Saıf. “Partial Characterization of Proteases Produced by Three Fungal Isolates from the Rhizosphere of Wild Yam Dioscorea Wallichii”. Journal of Applied Biological Sciences 3, sy. 3 (Kasım 2009): 71-75.
EndNote Sıvakumar N, Remya R, Saıf AB (01 Kasım 2009) Partial characterization of proteases produced by three fungal isolates from the rhizosphere of wild yam Dioscorea wallichii. Journal of Applied Biological Sciences 3 3 71–75.
IEEE N. Sıvakumar, R. Remya, ve A. B. Saıf, “Partial characterization of proteases produced by three fungal isolates from the rhizosphere of wild yam Dioscorea wallichii”, J.appl.biol.sci., c. 3, sy. 3, ss. 71–75, 2009.
ISNAD Sıvakumar, Nallusamy vd. “Partial Characterization of Proteases Produced by Three Fungal Isolates from the Rhizosphere of Wild Yam Dioscorea Wallichii”. Journal of Applied Biological Sciences 3/3 (Kasım 2009), 71-75.
JAMA Sıvakumar N, Remya R, Saıf AB. Partial characterization of proteases produced by three fungal isolates from the rhizosphere of wild yam Dioscorea wallichii. J.appl.biol.sci. 2009;3:71–75.
MLA Sıvakumar, Nallusamy vd. “Partial Characterization of Proteases Produced by Three Fungal Isolates from the Rhizosphere of Wild Yam Dioscorea Wallichii”. Journal of Applied Biological Sciences, c. 3, sy. 3, 2009, ss. 71-75.
Vancouver Sıvakumar N, Remya R, Saıf AB. Partial characterization of proteases produced by three fungal isolates from the rhizosphere of wild yam Dioscorea wallichii. J.appl.biol.sci. 2009;3(3):71-5.