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Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation

Yıl 2011, Cilt: 5 Sayı: 1, 55 - 58, 01.02.2011

Özge Karakaş Cüneyt Uçarlı Nermin Gözükırmızı Filiz Gürel

Öz

This study describes a protocol for Agrobacterium-mediated transformation of barley mature embryos. Cultured embryos were co-cultivated by EHA 105 strain carrying pCambia2301 binary vector and assayed for transient GUS expression. After 3 days of transformation, GUS expressing spots have been observed over 20.3% of total embryos. Among the tested several pre-treatments, infiltration in a vacuum chamber (400 mm Hg for 20 min) and infection by AGL-1 strain instead of EHA 105 increased the numbers of embryos with GUS staining significantly (p<0.05). PCR and Southern analyses showed that transformation occured in some of the T0 plants which were also tolerant to selective conditions

Anahtar Kelimeler

Hordeum vulgare L. Agrobacterium-mediated transformation mature embryo

Yıl 2011, Cilt: 5 Sayı: 1, 55 - 58, 01.02.2011

Özge Karakaş Cüneyt Uçarlı Nermin Gözükırmızı Filiz Gürel

Öz

Toplam 0 adet kaynakça vardır.

Ayrıntılar

Diğer ID JA67VK79MR
Bölüm Araştırma Makalesi
Yazarlar

Özge Karakaş Bu kişi benim

Cüneyt Uçarlı Bu kişi benim

Nermin Gözükırmızı Bu kişi benim

Filiz Gürel Bu kişi benim

Yayımlanma Tarihi 1 Şubat 2011
Yayımlandığı Sayı Yıl 2011 Cilt: 5 Sayı: 1

Kaynak Göster

APA Karakaş, Ö., Uçarlı, C., Gözükırmızı, N., Gürel, F. (2011). Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation. Journal of Applied Biological Sciences, 5(1), 55-58.
AMA Karakaş Ö, Uçarlı C, Gözükırmızı N, Gürel F. Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation. J.appl.biol.sci. Şubat 2011;5(1):55-58.
Chicago Karakaş, Özge, Cüneyt Uçarlı, Nermin Gözükırmızı, ve Filiz Gürel. “Assessment of Barley (Hordeum Vulgare L.) Mature Embryos for Agrobacterium-Mediated Transformation”. Journal of Applied Biological Sciences 5, sy. 1 (Şubat 2011): 55-58.
EndNote Karakaş Ö, Uçarlı C, Gözükırmızı N, Gürel F (01 Şubat 2011) Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation. Journal of Applied Biological Sciences 5 1 55–58.
IEEE Ö. Karakaş, C. Uçarlı, N. Gözükırmızı, ve F. Gürel, “Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation”, J.appl.biol.sci., c. 5, sy. 1, ss. 55–58, 2011.
ISNAD Karakaş, Özge vd. “Assessment of Barley (Hordeum Vulgare L.) Mature Embryos for Agrobacterium-Mediated Transformation”. Journal of Applied Biological Sciences 5/1 (Şubat 2011), 55-58.
JAMA Karakaş Ö, Uçarlı C, Gözükırmızı N, Gürel F. Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation. J.appl.biol.sci. 2011;5:55–58.
MLA Karakaş, Özge vd. “Assessment of Barley (Hordeum Vulgare L.) Mature Embryos for Agrobacterium-Mediated Transformation”. Journal of Applied Biological Sciences, c. 5, sy. 1, 2011, ss. 55-58.
Vancouver Karakaş Ö, Uçarlı C, Gözükırmızı N, Gürel F. Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation. J.appl.biol.sci. 2011;5(1):55-8.