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Yıl 2011, Cilt: 5 Sayı: 2, 33 - 39, 01.07.2011

Öz

Application of Immobilized Ipomoea batata β Amylase in the Saccharification of Starch

Yıl 2011, Cilt: 5 Sayı: 2, 33 - 39, 01.07.2011

Öz

Present study demonstrates the immobilization of acetone fractionated Ipomoea batata (sweet potato) β amylase on an inorganic support, Celite-545 by simple adsorption mechanism. The adsorbed enzyme exhibited an activity yield of 244 U g-1 of the matrix with effectiveness factor ‘η’ 0.83. Interaction between Celite-545 and enzyme was confirmed by fourier transform infrared spectroscopy and atomic force microscopy. The binding efficiency of enzyme to the support was analyzed by eluting it with 1.0 M NaCl. Both soluble and immobilized β amylase exhibited same pH optima while temperature optimum of immobilized enzyme was shifted from 50oC to 60oC. The immobilized β amylase preparation was superior to the free enzyme in hydrolyzing starch in a batch process: it hydrolyzed starch to 88% and 96% at 40oC and 50oC, respectively whereas soluble enzyme hydrolyzed only 83% and 80% of starch under similar experimental conditions. The immobilized β amylase retained 84% of its original activity after 30 days storage at 4oC, while the soluble enzyme showed only 41% of the initial activity under identical conditions. Immobilized β amylase retained 79% activity even after its 7th repeated use

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Ayrıntılar

Diğer ID JA94PR28FE
Bölüm Araştırma Makalesi
Yazarlar

Mohammad Jahir Khan Bu kişi benim

Fahim Halim Khan Bu kişi benim

Qayyum Husaın Bu kişi benim

Yayımlanma Tarihi 1 Temmuz 2011
Yayımlandığı Sayı Yıl 2011 Cilt: 5 Sayı: 2

Kaynak Göster

APA Khan, M. J., Khan, F. H., & Husaın, Q. (2011). Application of Immobilized Ipomoea batata β Amylase in the Saccharification of Starch. Journal of Applied Biological Sciences, 5(2), 33-39.
AMA Khan MJ, Khan FH, Husaın Q. Application of Immobilized Ipomoea batata β Amylase in the Saccharification of Starch. J.appl.biol.sci. Temmuz 2011;5(2):33-39.
Chicago Khan, Mohammad Jahir, Fahim Halim Khan, ve Qayyum Husaın. “Application of Immobilized Ipomoea Batata β Amylase in the Saccharification of Starch”. Journal of Applied Biological Sciences 5, sy. 2 (Temmuz 2011): 33-39.
EndNote Khan MJ, Khan FH, Husaın Q (01 Temmuz 2011) Application of Immobilized Ipomoea batata β Amylase in the Saccharification of Starch. Journal of Applied Biological Sciences 5 2 33–39.
IEEE M. J. Khan, F. H. Khan, ve Q. Husaın, “Application of Immobilized Ipomoea batata β Amylase in the Saccharification of Starch”, J.appl.biol.sci., c. 5, sy. 2, ss. 33–39, 2011.
ISNAD Khan, Mohammad Jahir vd. “Application of Immobilized Ipomoea Batata β Amylase in the Saccharification of Starch”. Journal of Applied Biological Sciences 5/2 (Temmuz 2011), 33-39.
JAMA Khan MJ, Khan FH, Husaın Q. Application of Immobilized Ipomoea batata β Amylase in the Saccharification of Starch. J.appl.biol.sci. 2011;5:33–39.
MLA Khan, Mohammad Jahir vd. “Application of Immobilized Ipomoea Batata β Amylase in the Saccharification of Starch”. Journal of Applied Biological Sciences, c. 5, sy. 2, 2011, ss. 33-39.
Vancouver Khan MJ, Khan FH, Husaın Q. Application of Immobilized Ipomoea batata β Amylase in the Saccharification of Starch. J.appl.biol.sci. 2011;5(2):33-9.