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Isolation and Molecular Identification of New Kluyveromyces lactis Strains Producing High Levels of Lactase and Invertase Enzymes

Yıl 2013, Cilt: 7 Sayı: 3, 51 - 55, 01.11.2013

Sezai Türkel Nükhet Kayakent

Öz

Six different new Kluyveromyces lactis strains were isolated from different dairy products from Bursa province, Turkey. Lactase and invertase enzyme activities of new K. lactis strains were determined under different growth conditions. Our results indicate that both lactase and invertase enzyme activities of new K. lactis strains are much higher than the standard K. lactis (ATCC8585). Lactase and invertase enzymes are expressed at high levels under glucose repressed growth conditions in some of these K. lactis strains. Invertase activities of new K. lactis strains are 10-fold higher than the standard K. lactis (ATCC8585) strain. Additionally, it was shown that the two of the new K. lactis strains have 25% shorter duplication times than the normal K. lactis (ATCC8585). Our results indicate that new K. lactis strains isolated from the local dairy products of the Bursa region of Turkey have distinct metabolic features with respect to the regulation of GAL/LAC regulon of K. lactis

Anahtar Kelimeler

Kluyveromyces lactis Lactase Invertase Yeast identification PCR-RFLP

Yıl 2013, Cilt: 7 Sayı: 3, 51 - 55, 01.11.2013

Sezai Türkel Nükhet Kayakent

Öz

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Ayrıntılar

Diğer ID JA32ZN53NK
Bölüm Araştırma Makalesi
Yazarlar

Sezai Türkel Bu kişi benim

Nükhet Kayakent Bu kişi benim

Yayımlanma Tarihi 1 Kasım 2013
Yayımlandığı Sayı Yıl 2013 Cilt: 7 Sayı: 3

Kaynak Göster

APA Türkel, S., & Kayakent, N. (2013). Isolation and Molecular Identification of New Kluyveromyces lactis Strains Producing High Levels of Lactase and Invertase Enzymes. Journal of Applied Biological Sciences, 7(3), 51-55.
AMA Türkel S, Kayakent N. Isolation and Molecular Identification of New Kluyveromyces lactis Strains Producing High Levels of Lactase and Invertase Enzymes. J.appl.biol.sci. Kasım 2013;7(3):51-55.
Chicago Türkel, Sezai, ve Nükhet Kayakent. “Isolation and Molecular Identification of New Kluyveromyces Lactis Strains Producing High Levels of Lactase and Invertase Enzymes”. Journal of Applied Biological Sciences 7, sy. 3 (Kasım 2013): 51-55.
EndNote Türkel S, Kayakent N (01 Kasım 2013) Isolation and Molecular Identification of New Kluyveromyces lactis Strains Producing High Levels of Lactase and Invertase Enzymes. Journal of Applied Biological Sciences 7 3 51–55.
IEEE S. Türkel ve N. Kayakent, “Isolation and Molecular Identification of New Kluyveromyces lactis Strains Producing High Levels of Lactase and Invertase Enzymes”, J.appl.biol.sci., c. 7, sy. 3, ss. 51–55, 2013.
ISNAD Türkel, Sezai - Kayakent, Nükhet. “Isolation and Molecular Identification of New Kluyveromyces Lactis Strains Producing High Levels of Lactase and Invertase Enzymes”. Journal of Applied Biological Sciences 7/3 (Kasım 2013), 51-55.
JAMA Türkel S, Kayakent N. Isolation and Molecular Identification of New Kluyveromyces lactis Strains Producing High Levels of Lactase and Invertase Enzymes. J.appl.biol.sci. 2013;7:51–55.
MLA Türkel, Sezai ve Nükhet Kayakent. “Isolation and Molecular Identification of New Kluyveromyces Lactis Strains Producing High Levels of Lactase and Invertase Enzymes”. Journal of Applied Biological Sciences, c. 7, sy. 3, 2013, ss. 51-55.
Vancouver Türkel S, Kayakent N. Isolation and Molecular Identification of New Kluyveromyces lactis Strains Producing High Levels of Lactase and Invertase Enzymes. J.appl.biol.sci. 2013;7(3):51-5.