SOME VARIETIES OF PATHOLOGICAL CHANGES IN EXPERIMENTAL INFECTION OF CARPS (Cyprinus carpio) WITH Aeromonas hydrophila

Abstract

The main clinical and pathomorphological changes in Aeromonas hydrophila infection in carp provoked by different field strains of this bacterium were studied. The strongest histopathological damages were seen in the functional epithelium of liver and kidneys, fol­lowed by intestine and hearth damages in addition to various haemorrhages in interstitial tissues of visceral organs and some skin haemorrhages on the ventral sur­face of the body and the anal region. As a whole, pathological damages consisted of degenerative changes as cloudy swelling, granular and/or hyaline droplet degeneration or vacuolation in functional epi­thelium in the respective internal organs in addition to some target vascular disturbances, which appeared to be good biomarkers for field assessment of that dis­ease. Pathological damages in internal organs and haemorrhages were stronger in experimental fishes exposed to higher levels of the respective pathogens, especially those isolated from dead anaconda or the referent strain, and less pronounced damages were seen in fishes infected with of Aeromonas hydrophila isolated from a silver carp with marked signs of septi­caemia.

Keywords

• Carp,Infection,Pathology,Pathomorpho¬logical changes,Aeromonas hydrophila

Kaynakça

1. Afifi, S.H., Al-Thobiati, S., Hazaa, M.S. (2000): Bacteriological and histopathological studies on Aeromonas hydrophila infection of Nile tilapia (Oreochromis niloticus) from fish farms in Saudi Arabia. Assiut Veterinary Medical Journal, 84: 195-205.
2. Alagappan, K.M., Deivasigamani, B., Kumaran, S., Sakthivel, M. (2009): Histopathological alterations in Estuarine Catfish (Arius macu¬latus; Thunberg, 1792) due to Aeronomas hydrophila infection. World Journal of Fish and Marine Sciences, 1(3): 185-189.
3. Alsaphar, S.A.A., Al-Faragi, J.K.H. (2012): De¬tection and study of the experimental infec¬tion of Aeromonas strain in the common carp (Cyprinus carpio L.), The Iraqi Journal of Veterinary Medicine, 36(2): 222– 230.
4. Angka, S.L. (1990): The pathology of the walk¬ing catfish, Clarias batrachus (L.), infected intraperitoneally with Aeromonas hydrophi¬la. Asian Fisheries Science, 3: 343-351.
5. Austin, B. and Austin, D. (1993): Bacterial fish pathogens. In: Disease in “Farmed and Wild Fish”, 2nd edition. Ellis Horwood, Chiches¬ter, United Kingdom, pp. 1-552.
6. Azad, I.S., Rajendran, K.V., Rajan, J.J.S., Vi¬jayan, K.K., Santiago T.C. (2001): Virulence and histopathology of Aeromonas hydrophi¬la (Sah 93) in experimentally infected tilap¬ia, Oreochromis mossambicus (L.). Journal of Aquaculture in the Tropics, 16(3): 265-275.
7. Dooley, J.S.G., Lallier, R., Trust, T.J. (1986): Surface antigens of virulent strains of Aer¬omonas hydrophila. Veterinary Immunology and Immunopathology, 12: 339-344.
8. Erer, H. (1981): The investigation of the patho¬logic findings of experimental Aeromonas hydrophila infection (Bacterial Haemorrhag¬ic Septicaernia) in carp, PhD thesis, Ankara University.

9. Hazen, T.C., Fliermans, C.B., Hirsch, R.P., Esch, G.W. (1978): Prevalence and distribution of Aeromonas hydrophila in the USA. Applied and Environmental Microbiology, 36: 731-738.
10. Horne, M.T., Baxendale, A. (1983): The adhe¬sion of Vibrio anguillarum to host tissues and its role in pathogenesis. Journal of Fish Disease, 6: 461-471.
11. Hoshina, R. (1962): Studies on red disease of Japanese eel. Journal of the Tokyo Universi¬ty of Fisheries, 6(1): 1-104.
12. Huizinga, H.W., Esch, G.W., Hazen, T.C. (1979): Histopathology of red-sore disease (Aer-omonas hydrophila) in naturally and exper¬imentally infected largemouth bass Microp¬terus salmoides (Lacépéde). Journal of Fish Disease, 2: 263-277.
13. Kanai, K., Wakabayashi, H. (1984): Purification and some properties of protease ftom Aer-omonas hydrophila. Bulletin of the Japanese Society for the Science of Fish, 50(8): 1367-1374.
14. Kanno, T., Nakai, T., Muroga, K. (1989). Mode of transmission of vibriosis among Ayu, Plecoglossus altivelis. Journal of Aquatic Animal Health, 1: 2-6.
15. Khalil, A.H., Mansour, E.H. (1997): Toxicity of crude extracellular products of Aeromonas hydrophila in tilapia, Tilapia nilotica. Let¬ters in Applied Microbiology, 25: 269-273.
16. Lallier, R., Daigneault, P. (1984): Antigenic dif¬ferentiation of pili from non-virulent and fish pathogenic strains of Aeromonas hy¬drophila. Journal of Fish Diseases 7: 509-572.
17. Miyazaki, T., Kubota, S.S. (1977): Histological studies of tissue and organs of fish and the application to fish pathologies, I. Vascular system of Japanese eel (Anguilla japonica). Bulletin of the Faculty of Fisheries Mie Uni¬versity, 4: 81-82.
18. Miyazaki, T., Jo, Y. (1985): A histophatological study of motile aeromonad disease in Ayu. Fish Pathology, 20(1): 55-59.
19. Miyazaki, T., Kaige, N. (1985): A histopatholog¬ical study on motile Aeromonad disease of Crucian carp. Fish Pathology, 21(3): 181-185.
20. Nemetz, T.G., Shotts, E.B. (1993): Zoonotic dis¬eases. In: Stoskopf, M.K. Saunders, W.B. (Eds.), Fish Medicine, Philadelphia, pp. 214-220.
21. Nieto, T.P., Santos, Y., Rodriguez, L.A., Ellis, A.E. (1991): An extracellular acetyl choline estrase produced by Aeromonas hydrophila is a major lethal toxin for fish. Microbial Pathogenesis, 11: 101-110.
22. Olivera, S.R., Souza, R.T.Y., Brasil, E.M., An¬drade, J.I.A., Nuness, É.S.S., Ono, E.A., Af¬fonso, E.G. (2011): LD50 of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus, Acta Amazonica, 41(2): 321 - 326
23. Palumbo, S., Abeyta, C., Stelma, G., Wesley, I.W., Wei, Ch., Koberger, J.A., Franklin, Sh.K., Schoeder-Tucker, L., Murano, E.A. (2001): Aeromonas, Acrobacter, and Plesi¬omonas. In: Downes, F.P., Ito, K.A. (Eds.), Compendium of methods for the microbio¬logical examination of foods. Fourth edition, American Public Health Association, Wash¬ington DC, pp. 283-300.
24. Rehulka, J. (2002): Aeromonas Causes Severe Skin Lesions in Rainbow Trout (Oncorhyn¬chus mykiss): Clinical Pathology, Haematol¬ogy and Biochemistry. Acta Veterinaria Brno, 71: 351-360.
25. Roberts, R.J. (1978): Fish Pathology. Billier Tin¬dall, London, pp. 318.
26. Rodriguez, L.A., Ellis, A.E., Nieto, T.P. (1992): Effects of acetylcholinesterase toxin of Aer-omonas hydrophila on the central nervous system of fish. Microbial Pathogenesis, 14: 411-415.
27. Thampuran, N., Surendran, P.K. (1995): Inci¬dence of motile aeromonads in marine envi-ronment, fishes and processed fishery prod¬ucts. Proceedings of the National Symposi¬um on Technological Advancements in Fisheries and its Impact on Rural Develop¬ment. School of Industrial Fisheries, Cochin University of Science and Technology, 5-7 Dec 1995, pp. 352-358.
28. Trust, T.J., Bull, M.L., Currie, B.R., Buckley, J.T. (1974): Obligate anaerobic bacteria in the gastrointestinal microflora of the grass carp (Ctenopharyngodon idella), goldfish (Carassius auratus), and rainbow trout (Salmo gairdneri). Journal of the Fisheries Research Board of Canada, 36: 1174-1179.
29. Ventura, M.T., Grizzle, J.M. (1988): Lesions as¬sociated with natural and experimental infections of Aeromonas hydrophila in channel catfish Ictalurus punctatus (Raf¬inesque). Journal of Fish Diseases, 11: 397-407.
30. Wakabayashi, H., Kanai, K., Hsu, I., Ecusa, S. (1981): Pathogenic activities of Aeromonas hydrophila biovar hydrophila (Chester) Popoff and Veron, 1976 to fishes. Fish Pa¬thology, 15(3/4): 319-325.
31. Yambot, A.V., Inglis, V. (1994): Aeromonas hy¬drophila isolated from Nile tilapia (Oreochromis niloticus) with "Eye Disease". In¬ternational Symposium on Aquatic Animal Health. University of California, School of Veterinary Medicine, Davis, CA, pp. 103.
32. Yardimci, B., Aydin, Y. (2011): Pathological findings of experimental Aeromonas hy¬drophila infection in Nile tilapia (Oreochromis niloticus). Ankara Üniversitesi Veteriner Fakultesi Dergisi. Veterinary Journal of Ankara University, 58: 47-54.