KÜLTÜR GÖKKUŞAĞI ALABALIĞI (Oncorhynchus mykiss)’NDAN İZOLE EDİLEN GRAM-NEGATİF PATOJENLERİN LİPOPOLİSAKKARİT PROFİLLERİ

Öz

Bu çalışma, Türkiye’nin farklı bölgelerindeki kültür gökkuşağı alabalıkları (Oncorhynchus mykiss)’ndan izole edilen patojenik Gram-negatif bakterilerin lipo­polisakkarit (LPS) profillerinin belirlenmesi amacıyla yürütülmüştür. Bu amaçla, hasta balık örneklerinden izole edilen Vibrio anguillarum, Aeromonas hydrophi­la, A. schubertii, Pseudomonas fluorescens ve Yersi­nia ruckeri izolatlarından LPS numuneleri elde edil­miştir. LPS profilleri, Sodyum Dodesil Sülfat-Poliakrilamid Jel Elektroforezi (SDS-PAGE) ve gümüş-nitrat boyama metodu ile belirlenmiştir. Bu çalışmadan elde edilen sonuçlar, LPS analizi çalışma­larının bu bakterilerin karakterizasyonunda ve değişik coğrafik bölgelerden izole edilen suşlar arasındaki farklılıkların tespit edilmesinde kullanılabilecek fay­dalı araçlar olduğunu göstermiştir.

Anahtar Kelimeler

• Gökkuşağı alabalığı,O. mykiss,SDS-PAGE,LPS

LIPOPOLYSACCHARIDE PROFILES OF GRAM-NEGATIVE PATHOGENS RECOVERED FROM CULTURED RAINBOW TROUT (Oncorhynchus mykiss)

Abstract

The aim of this study was to determine the lipopoly­saccharide (LPS) profiles of pathogenic Gram-negative bacteria recovered from cultured rainbow trout (Oncorhynchus mykiss) in different regions of Turkey. For this purpose, LPS samples were obtained from 10 bacterial isolates of Vibrio anguillarum, Aer­omonas hydrophila, A. schubertii, Pseudomonas fluo­rescens and Yersinia ruckeri recovered from diseased fish. LPS profiles were determined by using Sodium Dodecyl Sulphate Polyacrilamide Gel Electrophoresis (SDS-PAGE) and silver-nitrate staining method. Re­sults of the study demonstrated that LPS profiling is a useful tool for characterization of these bacteria and geographic discrimination of their isolates.

Keywords

• Rainbow trout,O. mykiss,SDS-PAGE,LPS

Kaynakça

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