PELİN OTU (Artemisia vulgaris L)’ NUN GÖKKUŞAĞI ALABALIKLARINDA (Oncorhynchus mykiss, Walbaum) HASTALIKLARA KARŞI DİRENÇ VE SPESİFİK OLMAYAN BAĞIŞIKLIK SİSTEMİ ÜZERİNE ETKİSİ

Öz

Bu araştırmada pelin otu (Artemisia vulgaris L)’ nun gökkuşağı alabalıklarında (Oncorhynchus mykiss, Walbaum) bağışıklık ve Vibrio anguillarum‘ a karşı direnç sağlayan uygun dozlarının tespiti amaçlanmıştır. Pelin otu, ortalama ağırlığı 20,48 ± 0,19--20,81 ±0,04 g olan balıkların yemlerine toz (%0, %0,1, %0,5, %1, %2) ve etanol ekstraktı (250 ve 1000 mg/kg) olarak ilave edilmiştir. Bazı hematolojik (hematokrit, eritrosit ve lökosit değerleri) ve immunolojik (lizozim, fagositik ve NBT aktivite) özellikler incelenmiştir. NBT, lizozim ve fagositik aktivitenin kontrol grubuna göre arttığı belirlenmiştir (p<0,05). Gökkuşağı alabalıkları, 45. günde V. anguillarum ile deneysel yolla enfekte edilmiştir. Araştırma sonuçlarımızda pelin otunun yemlere ilavesi ile balıkların, immun sistemi ve V. anguillarum’ a karşı direncinin arttığı belirlenmiştir. Pelin otunun balıklarda immunostimulant olarak kullanılabileceği sonucuna varılmıştır.

Anahtar Kelimeler

• Artemisia vulgaris,Vibrio anguillarum,Bağışıklık,Oncorhynchus mykiss,Lizozim,Fagositik aktivite

EFFECT OF WORMWOOD (Artemisia vulgaris L) ON RESISTANCE AGAINST DISEASES AND NONSPECIFIC IMMUNE SYSTEM IN RAİNBOW TROUT (Oncorhynchus mykiss, Walbaum)

Abstract

The present study was aimed at determining the effects of wormwood (Artemisia vulgaris L) on the immune response and disease resistance against Vibrio anguillarum in rainbow trout (Oncorhynchus mykiss Walbaum). Wormwood was incorporated into the diets as powder (0%, 0.1%, 0.5%, 1%, 2%) and ethanol extracts 250 and 1000 mg kg^-1 of rainbow trout O. mykiss (20.48 ± 0.19--20.81 ±0.04 g). Some haematological (WBC, RBC, level) and immunological (NBTassay, lysozyme and phagocytic activity) parameters were determined in fish blood. In NBTassay, lysozyme and phagocytic activity were enhanced in wormwood treated groups compared with the control group (p<0.05). After 45 days,  fish were challenged with V. anguillarum and mortality (%) was recorded up to day 10 post-challenge. The best survival (100%) was in the 1%  and 2% wormwood diet groups. These results indicate that wormwood supplementation significantly increased the immunity and makes O. mykiss more resistant to infection by V. anguillarum. It could be concluded that wormwood could be used as immunostimulant for rainbow trout.

Keywords

• Artemisia vulgaris,Vibrio anguillarum,Immunity,Oncorhynchus mykiss,Lysozyme,Phagocytic activity

Kaynakça

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