AKIM SİTOMETRİ VE SU ÜRÜNLERİ UYGULAMALARI

Yıl 2016, Cilt: 2 Sayı: 4, 159 - 175, 21.06.2016

Metin Yazıcı , Remziye Eda Yardımcı

https://doi.org/10.3153/JAEFR16018

Öz

Akım sitometri (AS), 0.2–150
µm büyüklüğündeki partiküllerin genellikle de hücrelerin tek tek, bir ışık demetinin önünden, bir sıvı
içinde geçerken, anlık olarak ölçümlerini yapan daha sonra da çoklu
fiziksel özelliklerini analiz eden bir teknolojidir. Akım sitometri, hücre biyolojisini
birçok açıdan araştırmak ve istenilen
hücreleri izole etmek için kullanılır. Akım sitometri çok sayıda tek hücrenin birçok karakteristiklerini hızlı bir şekilde
ölçtüğünden hücre tabanlı
analizde altın standart olarak
kabul edilmiştir. Diğer tekniklerle karşılaştırıldığında,
Akım sitometri’nin hızlı veri eldesini ve çok parametreli analizi kolaylaştırması popülaritesinin artmasına ve uygulama alanlarının genişlemesine yol
açmıştır. Bu derlemede, Akım sitometri’nin
su ürünleri alanındaki mevcut durumu ve uygulamaları hakkında özet bir şekilde
bilgi verilmiştir.

Anahtar Kelimeler

Akım Sitometri, Balıklarda fagositik aktivite, Hücre taban-lı analiz, Akuatik toksikoloji, Plankton, Hemosit

.FLOW CYTOMETRY AND ITS APPLICATIONS IN AQUTIC SCIENCES

Öz

Flow
cytometry (FCM) is a technology that simultaneously measures and then analyzes
multiple physical characteristics of 0.2–150 µm sized particles, usually cells,
as they flow one by one in a fluid stream through a beam of light. Flow cytometry
is used for investigating many aspects of cell biology and for isolating the
cells desired. Since Flow cytometry measures the multiple characteristics of
large numbers of individual cells rapidly, it has been accepted as the gold
Standard in cell based analysis. Since Flow cytometry facilitates rapid data
acquisition and eases multiparameter analysis, leading to increased popularity
and widespread applications as compared to other analyzing techniques. In this
review, the current status and utilization of Flow cytometry in aquatic sciences
are briefly presented.

Anahtar Kelimeler

Flow Cytometry, Phagositic activiy of fish, Cell-based analysis, Aquatic toxicology, Plankton, Haemocyte

Kaynakça

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