KÜLTÜR GÖKKUŞAĞI ALABALIĞI (Oncorhynchus mykiss)’NDAN İZOLE EDİLEN GRAM-NEGATİF PATOJENLERİN LİPOPOLİSAKKARİT PROFİLLERİ

Yıl 2015, Cilt: 1 Sayı: 2, 80 - 89, 02.02.2015

Tülay Akaylı Özgür Çanak Çiğdem Ürkü

https://doi.org/10.3153/JAEFR15008

Öz

Bu çalışma, Türkiye’nin farklı bölgelerindeki kültür gökkuşağı alabalıkları
(Oncorhynchus mykiss)’ndan izole
edilen patojenik Gram-negatif bakterilerin lipo­polisakkarit (LPS) profillerinin
belirlenmesi amacıyla yürütülmüştür. Bu amaçla, hasta balık örneklerinden izole
edilen Vibrio anguillarum, Aeromonas hydrophi­la, A. schubertii,
Pseudomonas fluorescens ve Yersi­nia
ruckeri izolatlarından LPS numuneleri elde edil­miştir. LPS profilleri, Sodyum
Dodesil Sülfat-Poliakrilamid Jel Elektroforezi (SDS-PAGE) ve gümüş-nitrat
boyama metodu ile belirlenmiştir. Bu çalışmadan elde edilen sonuçlar, LPS
analizi çalışma­larının bu bakterilerin karakterizasyonunda ve değişik coğrafik
bölgelerden izole edilen suşlar arasındaki farklılıkların tespit edilmesinde
kullanılabilecek fay­dalı araçlar olduğunu göstermiştir.

Anahtar Kelimeler

Gökkuşağı alabalığı, O. mykiss, SDS-PAGE, LPS

LIPOPOLYSACCHARIDE PROFILES OF GRAM-NEGATIVE PATHOGENS RECOVERED FROM CULTURED RAINBOW TROUT (Oncorhynchus mykiss)

Öz

The aim of this
study was to determine the lipopoly­saccharide (LPS) profiles of pathogenic
Gram-negative bacteria recovered from cultured rainbow trout (Oncorhynchus mykiss) in different
regions of Turkey. For this purpose, LPS samples were obtained from 10
bacterial isolates of Vibrio anguillarum, Aer­omonas hydrophila, A.
schubertii, Pseudomonas fluo­rescens
and Yersinia ruckeri recovered from
diseased fish. LPS profiles were determined by using Sodium Dodecyl Sulphate
Polyacrilamide Gel Electrophoresis (SDS-PAGE) and silver-nitrate staining
method. Re­sults of the study demonstrated that LPS profiling is a useful tool
for characterization of these bacteria and geographic discrimination of their
isolates.

Anahtar Kelimeler

Rainbow trout, O. mykiss, SDS-PAGE, LPS

Kaynakça

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