Biotechnological potential of apple pomace for value-added products

Yıl 2023, , 408 - 416, 29.06.2023

Sıla Sözgen Serpil Takaç

https://doi.org/10.31015/jaefs.2023.2.19

Öz

Agri-food processing waste and by-products are important to be valued in an integral unit to the main process. This study focused on showing the potential valorization of apple pomace as substrate towards valuable products by a biotechnological mean. Apple pomace was fermented by B.subtilis at 37 °C, 150 rpm, and 72 h. Reducing sugars, total phenol content and α-amylase activity were followed throughout the fermentation. The results showed that B.subtilis assimilated apple pomace sugars and stimulated the release of sugars into the medium during fermentation. α-amylase activity detected in the medium also indicated the degradation of pomace by B. subtilis. However, the total phenol content was found to be low. The α-amylase activity at 24th h was 29.6% higher when the fermentation initiated with a former fermentation medium than that of started with the inoculum based on agar and liquid incubation media. Overall results showed –for the first time- that apple pomace can be valued towards α-amylase activity, reducing sugar and total phenol content by the activity of B.subtilis cells.

Anahtar Kelimeler

Apple pomace valorization, Fermentation, Bacillus subtilis, α-amylase enzyme

Teşekkür

The first author was awarded a Scholarship by The Scientific and Technological Research Council of Turkey (TÜBİTAK) 2210-D National Scholarship Programme for MSc Students.

Kaynakça

• Ajila, C.M., Brar, S.K., Verma, M., Tyagi, R.D., Valéro, J.R., 2011. Solid-state fermentation of apple pomace using Phanerocheate chrysosporium – Liberation and extraction of phenolic antioxidants. Food Chem. 126, 1071–1080. https://doi.org/10.1016/j.foodchem.2010.11.129
• Ajila, C.M., Sarma, S.J., Brar, S.K., Godbout, S., Cote, M., Guay, F., Verma, M., Valéro, J.R., 2015. Fermented Apple Pomace as a Feed Additive to Enhance Growth Performance of Growing Pigs and Its Effects on Emissions. Agriculture 5, 313–329. https://doi.org/10.3390/agriculture5020313
• Bialkowska, A.M., Jędrzejczak-Krzepkowska, M., Gromek, E., Krysiak, J., Sikora, B., Kalinowska, H., Kubik, C., Schütt, F., Turkiewicz, M., 2016. Effects of genetic modifications and fermentation conditions on 2 , 3-butanediol production by alkaliphilic Bacillus subtilis. Appl Microbiol Biotechnol 2663–2676. https://doi.org/10.1007/s00253-015-7164-2
• Dash, B.K., Rahman, M.M., Sarker, P.K., 2015. Molecular identification of a newly isolated bacillus subtilis BI19 and optimization of production conditions for enhanced production of extracellular amylase. Biomed Res. Int. 2015, 9 pages. https://doi.org/10.1155/2015/859805
• Enzymatic Assay of α-Amylase (EC 3.2.1.1) [WWW Document], n.d. URL https://www.s_gmaaldr_ch.com/techn_cal-documents/protocols/b_ology/enzymat_c-assay-of-a-amylase.html
• Gassara, F., Ajila, C.M., Brar, S.K., Verma, M., Tyagi, R.D., Valero, J.R., 2012. Liquid state fermentation of apple pomace sludge for the production of ligninolytic enzymes and liberation of polyphenolic compounds. Process Biochem. 47, 999–1004. https://doi.org/https://doi.org/10.1016/j.procbio.2012.03.001
• Keskin-Šašić, I., Tahirović, I., Topčagić, A., Klepo, L., Salihović, M., Ibragić, S., Toromanović, J., Ajanović, A., Velispahić, E., 2012. Total Phenolic Content and Antioxidant Capacity of Fruit Juices. Bull. Chem. Technol. Bosnia Herzegovina 39, 25–28.
• Kosseva, M., 2011. Management and Processing of Food Wastes, Second edi. ed, Comprehensive Biotechnology. Reference Module in Earth Systems and Environmental Sciences. https://doi.org/https://doi.org/10.1016/B978-0-08-088504-9.00393-7
• Kumar, P., Ray, S., Kalia, V.C., 2016. Production of co-polymers of polyhydroxyalkanoates by regulating the hydrolysis of biowastes. Bioresour. Technol. 200, 413–419. https://doi.org/http://dx.doi.org/10.1016/j.biortech.2015.10.045
• Kuvvet, C., Uzuner, S., Cekmecelioglu, D., 2017. Improvement of Pectinase Production by Co-culture of Bacillus spp. Using Apple Pomace as a Carbon Source. Waste and Biomass Valorization 0, 1–9. https://doi.org/10.1007/s12649-017-0142-4
• Lyu, F., Luiz, S.F., Azeredo, D.R.P., Cruz, A.G., Ajlouni, S., Ranadheera, C.S., 2020. Apple pomace as a functional and healthy ingredient in food products: A review. Processes 8, 1–15. https://doi.org/10.3390/pr8030319
• Miller, G.L., 1959. Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar. Anal. Chem. 31, 426–428. https://doi.org/10.1021/ac60147a030
• Rana, N., Verma, N., Vaidya, D., Dipta, B., 2017. Production of Amylase from Bacillus thuringiensis J2 Using Apple Pomace as Substrate in Solid State Fermentation. Int. J. Curr. Microbiol. Appl. Sci. 6, 3465–3474. https://doi.org/https://doi.org/10.20546/ijcmas.2017.608.415
• Sadasivam S., M.A., 2008. Biochemical Methods, Third Edit. ed. New Age International Publishers, New Delhi. Sanger, F., Nicklen, S., Coulson, A.., 1977. DNA sequencing with chain-terminating. Proc Natl Acad Sci USA 74, 5463–5467.
• Savatovic, S., Tepic, A., Sumic, Z., Nikolic, M., 2009. Antioxidant activity of polyphenol-enriched apple juice. Acta Period. Technol. 220, 95–102. https://doi.org/10.2298/apt0940095s
• Škerget, M., Kotnik, P., Hadolin, M., Hraš, A.R., Simonič, M., Knez, Ž., 2005. Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities. Food Chem. 89, 191–198. https://doi.org/10.1016/j.foodchem.2004.02.025
• Su, Y., Liu, C., Fang, H., Zhang, D., 2020. Bacillus subtilis: A universal cell factory for industry, agriculture, biomaterials and medicine. Microb. Cell Fact. 19, 1–12. https://doi.org/10.1186/s12934-020-01436-8
• Sulewska, H., Adamczyk, J., Gygert, H., Rogacki, J., Szymanska, G., Smiatacz, K., Panasiewicz, K., Tomaszyk, K., 2014. A comparison of controlled self-pollination and open pollination results based on maize grain quality. Spanish J. Agric. Res. 12, 492–500. https://doi.org/10.5424/sjar/2014122-4970
• Sun, H., Ge, X., Hao, Z., Peng, M., 2010. Cellulase production by Trichoderma sp . on apple pomace under solid state fermentation. African J. Biotechnol. 9, 163–166.
• Venkata, E., Raju, N., Goli, D., 2014. Effect Of Physiochemical Parameters On Fibrinolytic Protease Production By Solid State Fermentation. Mater. Sci. 3, 1937–1954.
• Yen, G., Chen, H., 1995. Antioxidant Activity of Various Tea Extracts in Relation to Their Antimut agenicity. J. Agric. Food Chem. 27–32.