Determination of sugars and organic acids in diverse carob genotypes using HPLC techniques
Abstract
Carob fruit is widely known for their abundance of health-boosting compounds like polyphenols, ascorbic acid, organic acids, and micronutrients. These compounds offer various benefits, including antioxidative, antimicrobial, antidiabetic, liver-protective, anti-inflammatory, anticancer, and heart-protective effects. In this research, High-Performance Liquid Chromatography (HPLC) methods were employed to assess sugar and organic acid levels in mature fruit pods of three distinct carob genotypes from the Kahramanmaras region in Turkey. The findings revealed that genotype-2 had the highest concentrations of glucose (1301 mg/100g) and fructose (875 mg/100g), genotype-3 exhibited the highest level of xylose (1548 mg/100g), while genotype-1 displayed the highest levels of sucrose (9183 mg/100g) and total sugars (12457 mg/100g). Regarding organic acids, genotype-1 exhibited the highest levels of oxalic acid (17.62 mg/100g), citric acid (612.50 mg/100g), and fumaric acid (8.0 mg/100g), while genotype-3 showed the highest levels of malic acid (234.92 mg/100g) and succinic acid (1089.76 mg/100g); however, genotype-2 had the highest amount of ascorbic acid (8.17 mg/100g). In conclusion, genotype-1 demonstrated the most favorable performance in terms of having the highest levels of total sugar and organic acids compared to the other two genotypes.
Keywords
Supporting Institution
Çukurova University
References
- Ashoor, S. H., & Knox, M. J. (1984). Determination of organic acids in foods by high-performance liquid chromatography: citric acid. Journal of Chromatography A, 299, 288-292. https://doi.org/10.1016/S0021-9673(01)97843-4
- Avallone, R., Plessi, M., Baraldi, M., & Monzani, A. (1997). Determination of chemical composition of carob (Ceratonia siliqua): protein, fat, carbohydrates, and tannins. Journal of food composition and analysis, 10(2), 166-172. https://doi.org/10.1006/jfca.1997.0528
- Ayaz, F. A., Torun, H., Ayaz, S. E. M. A., Correia, P. J., Alaiz, M., Sanz, C., ... & Strnad, M. (2007). Determination of chemical composition of anatolian carob pod (Ceratonia siliqua L.): sugars, amino and organic acids, minerals and phenolic compounds. Journal of food quality, 30(6), 1040-1055. https://doi.org/10.1111/j.1745-4557.2007.00176.x
- Bates, S. H., Jones, R. B., & Bailey, C. J. (2000). Insulin‐like effect of pinitol. British journal of pharmacology, 130(8), 1944-1948. ,https://doi.org/10.1038/sj.bjp.0703523
- Battle, I., & Tous, J. (1997). Carob Tree (Ceratonia siliqua L.), International Plant Genetic Resources Institute. Via delle Sette Chiese, 142, 00145. https://hdl.handle.net/10568/104277
- Biner, B., Gubbuk, H. A. M. İ. D. E., Karhan, M. U. S. T. A. F. A., Aksu, M., & Pekmezci, M. (2007). Sugar profiles of the pods of cultivated and wild types of carob bean (Ceratonia siliqua L.) in Turkey. Food chemistry, 100(4), 1453-1455. https://doi.org/10.1016/j.foodchem.2005.11.037
- Bozan, B., Başer, K. H. C., & Kara, S. (1997). Quantitative determination of naphthaquinones of Arnebia densiflora (Nordm.) Ledeb. by an improved high-performance liquid chromatographic method. Journal of Chromatography A, 782(1), 133-136. https://doi.org/10.1016/S0021-9673(97)00460-3
- Brassesco, M. E., Brandao, T. R., Silva, C. L., & Pintado, M. (2021). Carob bean (Ceratonia siliqua L.): A new perspective for functional food. Trends in Food Science & Technology, 114, 310-322. https://doi.org/10.1016/j.tifs.2021.05.037
- Cheng, G. W., & Crisosto, C. H. (1997). Iron—polyphenol complex formation and skin discoloration in peaches and nectarines. Journal of the American Society for Horticultural Science, 122(1), 95-99. https://doi.org/10.21273/JASHS.122.1.95
- Correia, P. J., & Martins-Loução, M. A. (2005). The use of macronutrients and water in marginal Mediterranean areas: the case of carob-tree. Field Crops Research, 91(1), 1-6. https://doi.org/10.1016/j.fcr.2004.05.004
