Effects of different explant and growth regulator combinations on in vitro propagation of Poinsettia (Euphorbia pulcherrima Willd.)
Abstract
The aim of the research is to develop a suitable micropropagation protocol for the Poinsettia (Euphorbia pulcherrima Willd.) plant, which is used as an ornamental plant. In the sterilization step, mercuric chloride together with sodium hypochlorite is approved as the best option. Thus, contamination of explants is eliminated. At the shoot propagation stage, BA is absolutely necessary as all doses of BA increased shoot propagation compared to the control. The highest number of shoots per explant (2.62 shoots/explant) and the longest shoots (2.16 cm) were obtained from 1.0 mg.L-1 BA medium. The highest number of leaves (20.41 leaves/explant) was obtained from 2.0 mg.L-1 BA medium. Kinetin was also effective when adding different concentrations to the culture medium compared to the control. In general, BA is more effective than Kinetin in these parameters. Kinetin, on the other hand, performed much better than BA in terms of shoot number. On the other hand, the highest rooting percentage (58.2%) was obtained from the addition of 0.3 mg.L-1 NAA. The highest root number (5.10 roots/explant) was obtained by adding 0.1 mg.L-1 NAA. Good performance was found in the acclimatization phase with plantlets transferred to the soil with a high survival rate reaching 100%. Most of the plantlets started growing well. The plantlets grew well and did not show morphological abnormalities. In addition, a successful plant regeneration was achieved by adding 1.0 mg.L-1 BA and 0.5 mg.L-1 NAA on the callus produced in leaf disc explants and a very good organogenesis was determined in terms of roots and shoots.
Keywords
Poinsettia, Euphorbia pulcherrima Willd., in vitro, Micropropagation, rRgeneration
References
- Babaoğlu, M., Gürel, E. & Özcan S. (2001). Bitki Biyoteknolojisi I. Doku Kültürü Uygulamaları. Selçuk Basımevi, 374.
- Bidarigh, S. & Azarpour, E. (2013). Study effect of MS medium and shoot tip, Biotechnology,17(2), 137-147.
- Castellanos, M., Power, J.B. & Davey, M.R. (2010). Micropropagation of poinsettia by organogenesis. In Protocols for in vitro propagation of ornamental plants (pp. 67-75). Humana Press. https://doi.org/10.1007/978-1-60327-114-1_7
- Clarke, J.L., Spetz, C., Hauglien, S., Xıng, S., Dees, M.W., Moe, R. & Blystad, D.R. (2008). Agrobacterium tumefaciens-mediated transformation of poinsettia,Euphorbia pulcherrima, with virus-derived hairpin RNA constructs confersresistance to Poinsettia mosaic virus. Plant Cell Reports,27(6), 1027-1038. https://doi.org/10.1007/s00299-008-0526-9
- George, M.W. (2008). The elements of library investigation. Princeton University Press.
- Mwine, T.J. & Van Damme, P. (2011). Evaluation of pesticidal properties ofEuphorbia tirucalli L.(Euphorbiaceae) against selected pests. Afrika Focus, 24(1), 119-121. https://doi.org/10.1163/2031356X-02401014
- Ogras, T.T., Tahtasakal, E. & Ozturk, S. (2022). In vitro production of tropane alkaloids from Brugmansia suaveolens. Int. J. Sec. Metabolite, Vol. 9, No. X, (2022) pp. 1-13. https://doi.org/10.21448/ijsm.934222
- Perera, D. & Trader, B.W. (2008). Poinsettia (Euphorbia pulcherrima) in vitro propagation. In Combined Proceedings International Plant PropagatorsSociety (Vol. 58, pp. 578-582).
- Perera, D. & Trader, B.W. (2010). Poinsettia ‘Prestige™ Red’(Euphorbiapulcherrima) in vitro propagation. Hort. Science, 45(7), 1126-1128. https://doi.org/10.21273/HORTSCI.45.7.1126
- Pickens, K.A., Cheng, Z.M. & Trigiano, R.N. (2005). Axillary bud proliferation and organogenesis of Euphorbia pulcherrima Winter Rose TM. İn vitro Cell DevBio-Plant 41: 770–774. https://doi.org/10.1079/IVP2005706
