Antagonistic activities of mycoparasitic Pythium species against Fusarium oxysporum f. sp. lycopersici and Botrytis cinerea on tomatoes

Meryem Ateş; Gürsel Hatat Karaca

doi:10.31015/jaefs.2024.1.18

EN

Antagonistic activities of mycoparasitic Pythium species against Fusarium oxysporum f. sp. lycopersici and Botrytis cinerea on tomatoes

Abstract

In this study, antagonistic effects of Pythium acanthophoron, P. lycopersicum, P. oligandrum and P. paroecandrum against Fusarium oxysporum f. sp. lycopersici and Botrytis cinerea were investigated by in vitro and in vivo trials. In vitro mycoparasitic activities of Pythium species were determined by dual culture, inverted plate culture and agar diffusion tests. As a result of dual culture tests, suppressive effects of all mycoparasites were over 70% against mycelial growth of the pathogens. Inverted plate tests showed that antagonistic effects of mycoparasites regarding their volatile compounds were rather low. In the agar diffusion test, all mycoparasites showed antibiosis effect, however P. lycopersicum had the highest suppressive effect on both pathogens. In pot trials, mycoparasites were effective to protect tomato seedlings when pathogens were seperately inoculated, and suppressed the symptoms. When two pathogens were inoculated together, P. paroecandrum was ineffective against B. cinerea, but decreased the severity of wilt symptoms, while other mycoparasites totally inhibited both diseases. Chromatographic analyses made by using leaf samples taken 12, 24, 48 and 72 hours after pathogen inoculation showed meaningful increase on chlorogenic acid, caffeic acid and epicatechine, in the samples taken 48 hours after inoculation. Analyses after the inoculations of tomatoes with the mycoparasites and/or pathogens showed that mycoparasites also caused increase in the amounts of phenolics. This indicated that the mycoparasites could be effective to induce defense mechanisms of tomato plants against pathogens. Among them, P. oligandrum can be mentioned as the most effective mycoparasite regarding the induction of phenolics.

Keywords

Solanum lycopersicum, Fusarium wilt, Gray mold, Biocontrol, Pythium spp.

Supporting Institution

Süleyman Demirel University

Project Number

2078-D-09

Ethical Statement

Ethics committee approval is not required since the article does not contain studies with human or animals.

Thanks

The authors are grateful to the Scientific Research Projects Coordination Unite of Süleyman Demirel University, for the financial support.

References

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Details

Primary Language

English

Subjects

Phytopathology

Journal Section

Research Article

Authors

Meryem Ateş
0000-0003-1556-4207
Türkiye

Gürsel Hatat Karaca ^*
0000-0002-5159-2734
Türkiye

Publication Date

March 25, 2024

Submission Date

January 22, 2024

Acceptance Date

March 6, 2024

Published in Issue

Year 2024 Volume: 8 Number: 1

DOI

https://doi.org/10.31015/jaefs.2024.1.18

IZ

https://izlik.org/JA26HG76AC

APA

Ateş, M., & Hatat Karaca, G. (2024). Antagonistic activities of mycoparasitic Pythium species against Fusarium oxysporum f. sp. lycopersici and Botrytis cinerea on tomatoes. International Journal of Agriculture Environment and Food Sciences, 8(1), 176-185. https://doi.org/10.31015/jaefs.2024.1.18

AMA

1.Ateş M, Hatat Karaca G. Antagonistic activities of mycoparasitic Pythium species against Fusarium oxysporum f. sp. lycopersici and Botrytis cinerea on tomatoes. int. j. agric. environ. food sci. 2024;8(1):176-185. doi:10.31015/jaefs.2024.1.18

Chicago

Ateş, Meryem, and Gürsel Hatat Karaca. 2024. “Antagonistic Activities of Mycoparasitic Pythium Species Against Fusarium Oxysporum F. Sp. Lycopersici and Botrytis Cinerea on Tomatoes”. International Journal of Agriculture Environment and Food Sciences 8 (1): 176-85. https://doi.org/10.31015/jaefs.2024.1.18.

EndNote

Ateş M, Hatat Karaca G (March 1, 2024) Antagonistic activities of mycoparasitic Pythium species against Fusarium oxysporum f. sp. lycopersici and Botrytis cinerea on tomatoes. International Journal of Agriculture Environment and Food Sciences 8 1 176–185.

IEEE

[1]M. Ateş and G. Hatat Karaca, “Antagonistic activities of mycoparasitic Pythium species against Fusarium oxysporum f. sp. lycopersici and Botrytis cinerea on tomatoes”, int. j. agric. environ. food sci., vol. 8, no. 1, pp. 176–185, Mar. 2024, doi: 10.31015/jaefs.2024.1.18.

ISNAD

Ateş, Meryem - Hatat Karaca, Gürsel. “Antagonistic Activities of Mycoparasitic Pythium Species Against Fusarium Oxysporum F. Sp. Lycopersici and Botrytis Cinerea on Tomatoes”. International Journal of Agriculture Environment and Food Sciences 8/1 (March 1, 2024): 176-185. https://doi.org/10.31015/jaefs.2024.1.18.

JAMA

1.Ateş M, Hatat Karaca G. Antagonistic activities of mycoparasitic Pythium species against Fusarium oxysporum f. sp. lycopersici and Botrytis cinerea on tomatoes. int. j. agric. environ. food sci. 2024;8:176–185.

MLA

Ateş, Meryem, and Gürsel Hatat Karaca. “Antagonistic Activities of Mycoparasitic Pythium Species Against Fusarium Oxysporum F. Sp. Lycopersici and Botrytis Cinerea on Tomatoes”. International Journal of Agriculture Environment and Food Sciences, vol. 8, no. 1, Mar. 2024, pp. 176-85, doi:10.31015/jaefs.2024.1.18.

Vancouver

1.Meryem Ateş, Gürsel Hatat Karaca. Antagonistic activities of mycoparasitic Pythium species against Fusarium oxysporum f. sp. lycopersici and Botrytis cinerea on tomatoes. int. j. agric. environ. food sci. 2024 Mar. 1;8(1):176-85. doi:10.31015/jaefs.2024.1.18

Cited By

In vitro assessment of the biocontrol activities of some Trichoderma species in suppressing Fusarium oxysporum f. sp. lycopersici

Frontiers in Life Sciences and Related Technologies

https://doi.org/10.51753/flsrt.1809615

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Abstract

Project Number

Antagonistic activities of mycoparasitic Pythium species against Fusarium oxysporum f. sp. lycopersici and Botrytis cinerea on tomatoes

Abstract

Keywords

Supporting Institution

Project Number

Ethical Statement

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

In vitro assessment of the biocontrol activities of some Trichoderma species in suppressing Fusarium oxysporum f. sp. lycopersici

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