Molecular characterization of Turkish hazelnut cultivars and genotypes using SSR markers
Abstract
This study defines the genetic characterization of 16 hazelnut varieties and 64 genotypes. SSR method was used in molecular characterization studies. 18 SSR primers were used. In the SSR method, 118 bands were obtained, and 115 were polymorphic. The similarity coefficient in overall genotypes were between 0.12 and 0.98 in SSR andthe polymorphism rate of the primer pairs used was calculated as 98.0%. The lowest polymorphism information content value (0.542) was obtained from the CAC- C 028 locus, and the highest polymorphism information content value (0.987) was obtained from the CAC- B 113 locus. The average polymorphism information content value was 0.786. The total discrimination power values of SSR loci were determined as 17.98; the lowest (0.748) discrimination power value was obtained from the CAC- B 020 locus, while the highest (1.404) discrimination power value was obtained from the CAC- A 024 b locus. The genotypes’clustering positions across the dendograms were essentially correlated with their geographic origins. This result shows that genetic origin plays an important role in terms of classification of genotypes in hazelnut.
Keywords
References
- Ayfer, M., Uzun, A. ve Baş, F., (1986). Türk Fındık Çeşitleri. Karadeniz Bölgesi Fındık İhracatçılar Birliği Yayınları, Ankara, 95 s(in Turkish).
- Bassil, N.V., Botta, R., Mehlenbacher, S.A. (2003). Microsatellite Markers Of The European Hazelnut. Hortscience. 38(5):Abstract P.740-741.
- Boccaccı, P., Akkak, A., Botta, R., (2006). DNA Typing and Genetic Relations Among European Hazelnut (Corylus avellena) Cultivars Using Microsatellite Markers. Genome. 49. 598.
- Doyle, J.J., Doyle, J.J., (1990). Isolation of Plant DNA from Fresh Tissue. Focus. 12: 13 – 15.
- Fiore, M C., Marchese A., Mauceri A., Digangl I., Scialabba, A.(2022). Diversity Assessment and DNA-Based Fingerprinting of Sicilian Hazelnut (Corylus avellana L.) Germplasm. Plants 2022, 11, 631. https://doi.org/10.3390
- Gökırmak, T., Mahlenbacher, S.A., Bassil, N.V., (2008). Characterization of European Hazelnut ( Corylus avellena) Cultivars Using SSR Markers. Gent Resour Crop Evol. ( doi:10.1007/s10722- 088-9352-8)
- HRI, (2024). Hazelnut Research Institute. https://arastirma.tarimorman.gov.tr/findik/Sayfalar/EN/AnaSayfa.aspx (accessed date 07.01.2024)
- İslam, A., ve Özgüven, A.I. (1997).Türkiye’de Fındık Yetiştiriciliği. Çukurova Üniversitesi Ziraat Fakültesi Dergisi, 12 (4): 165 – 174 (in Turkish).
- İslam, A., (2000). Clonal selection in Turkish hazelnut cultivars grown in Ordu. PhD Thesis, Çukurova University, Graduate School of Natural and Applied Sciences, Department of Horticulture, Adana, Türkiye 192 s.
- Kaçar Aka, Y. (2001). Classification of some sweet (p. avium L. ) and sourcherry (p. cerasus L. ) varieties and types grown in Turkey by using DNA fingerprinting. PhD Thesis, Çukurova University, Graduate School of Natural and Applied Sciences, Department of Horticulture, Adana, Türkiye 192 s.
