Research Article

Shoot tip culture of Bilecik İrikarası, Sarı Üzüm, Kartal Çavuş and Razakı grape varieties grown in Bilecik province

Volume: 8 Number: 3 September 29, 2024
Seda Özdemir Memiş , Hayri Sağlam *
EN

Shoot tip culture of Bilecik İrikarası, Sarı Üzüm, Kartal Çavuş and Razakı grape varieties grown in Bilecik province

Abstract

The aim of this study was to determine the propagation potential of some local varieties using the in vitro shoot-tip culture method. Bilecik İrikarası, Sarı Üzüm, Kartal Çavuş and Razakı were used as materials. As a result of the study, the values of rooting rate, number of roots, length of roots, number of shoots, length of shoots, number of leaves and number of nodes of the grape varieties were determined. When the cultivars were compared in terms of rooting characteristics, the highest rooting rate was 54.7%, the highest root number was 3.71 per plant, and the highest average root length value was 17.93 cm from Bilecik İrikarası. When the cultivars were evaluated in terms of shoot length, the highest shoot length value was determined in Razaki variety with 5.72 cm. Similarly, considering the number of leaves and nodes in the shoots were determined, with the highest leaf number value was of 8.71, the highest node number value was of 6.71 in Razakı variety. As a result of the study, local varieties that used as material in this study showed positive results in in vitro propagation.

Keywords

Bilecik, Grape, Tissue Culture, Local Variety, Shoot Tip Culture

Supporting Institution

This study was supported by Scientific Research Projects Coordination Office of Bilecik Şeyh Edebali University.

Project Number

2019-01.BŞEÜ.06-01

Thanks

We thank to Scientific Research Projects Coordination Office of Bilecik Şeyh Edebali University.

References

  1. Aazami M.A., 2010. Effect of some growth regulators on “in vitro” culture of two Vitis vinifera L. Cultivars. Romanian Biotechnological Letters, Vol. 15, No.3; 5229-5232, 2010
  2. Adıyaman F., 1998. Vitis Vinifera L.Cv. Alphonse’ un Gövdelerini, Çiçek Durumlarının Ve Lateral Tomurcuklarının Proliferasyon Kapasitelerinin, Yılın Değişik Zamanlarına Göre İn Vitro Şartlarda Karşılaştırılması Araştırmaları. Yüksek Lisans Tezi (Basılmamış) Dicle Üniversitesi Fen Bilimleri Enstitüsü, 66 s.
  3. Anonymus, 2024. İllere Ait Mevsim Normalleri (1991-2020). https://www.mgm.gov.tr/veridegerlendirme/il-ve-ilceler-istatistik.aspx?k=H&m=BILECIK
  4. Ayman A. D., Khalil S. M. & Roba M. I., 2011. Regeneration and Micropropagation of Grapevine (Vitis vinifera L.) Through Shoot Tips and Axillary Buds. International Journal of Advanced Biotechnology and Research ISSN 0976-2612, Vol 2, Issue 4, 2011, pp 484-491
  5. Balı, E. A., Dardeniz, A., Baytekin, G., Şahin, E. & Türkmen, O. S., 2020. Bazı Üzüm Çeşitlerinin Doku Kültürü Yöntemiyle Mikroçoğaltımı Üzerine Bir Araştırma. Cilt 1 Sayı 2, 30-35
  6. Batukaev A., Sobralieva E. & Palaeva D., 2021. Optimization Studies of Culture Media for In-Vitro Clonal Micropropagation of New Grape Varieties. in International research conference on Challenges and Advances in Farming, Food Manufacturing, Agricultural Research and Education, KnE Life Sciences, pages 757–764. DOI 10.18502/kls.v0i0.9013
  7. Baydar, N. G., 2000. Asmada (Vitis spp.) Yapraklardan adventif sürgün oluşumu üzerine bir araştırma. Turkish Journal of Biology, 24(3), 645-656.
  8. Beza, K. (2010). Multiple shoot regeneration study on three varieties of grape vine (Vitis vinifera L.) from shoot tip and nodal culture (Doctoral dissertation, Addis Ababa University).
  9. Ekbiç, H. B. & Yılmaz, G. (2018). Kokulu Kara Üzümün (Vitis labrusca L.) Mikro Çelik Kültürü ile Mikro Çoğaltımı. Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 28(1), 86-91.
  10. Ekbiç, H. B., Yılmaz, G. Ş. & Ciğerli, S., 2015. Isabella (Vitis labrusca) üzüm çeşidinin in vitro sürgün ucu kültürü ile çoğaltılması. Akademik Ziraat Dergisi, 4(2), 65-70.
APA
Özdemir Memiş, S., & Sağlam, H. (2024). Shoot tip culture of Bilecik İrikarası, Sarı Üzüm, Kartal Çavuş and Razakı grape varieties grown in Bilecik province. International Journal of Agriculture Environment and Food Sciences, 8(3), 729-735. https://doi.org/10.31015/jaefs.2024.3.26
AMA
1.Özdemir Memiş S, Sağlam H. Shoot tip culture of Bilecik İrikarası, Sarı Üzüm, Kartal Çavuş and Razakı grape varieties grown in Bilecik province. int. j. agric. environ. food sci. 2024;8(3):729-735. doi:10.31015/jaefs.2024.3.26
Chicago
Özdemir Memiş, Seda, and Hayri Sağlam. 2024. “Shoot Tip Culture of Bilecik İrikarası, Sarı Üzüm, Kartal Çavuş and Razakı Grape Varieties Grown in Bilecik Province”. International Journal of Agriculture Environment and Food Sciences 8 (3): 729-35. https://doi.org/10.31015/jaefs.2024.3.26.
EndNote
Özdemir Memiş S, Sağlam H (September 1, 2024) Shoot tip culture of Bilecik İrikarası, Sarı Üzüm, Kartal Çavuş and Razakı grape varieties grown in Bilecik province. International Journal of Agriculture Environment and Food Sciences 8 3 729–735.
IEEE
[1]S. Özdemir Memiş and H. Sağlam, “Shoot tip culture of Bilecik İrikarası, Sarı Üzüm, Kartal Çavuş and Razakı grape varieties grown in Bilecik province”, int. j. agric. environ. food sci., vol. 8, no. 3, pp. 729–735, Sept. 2024, doi: 10.31015/jaefs.2024.3.26.
ISNAD
Özdemir Memiş, Seda - Sağlam, Hayri. “Shoot Tip Culture of Bilecik İrikarası, Sarı Üzüm, Kartal Çavuş and Razakı Grape Varieties Grown in Bilecik Province”. International Journal of Agriculture Environment and Food Sciences 8/3 (September 1, 2024): 729-735. https://doi.org/10.31015/jaefs.2024.3.26.
JAMA
1.Özdemir Memiş S, Sağlam H. Shoot tip culture of Bilecik İrikarası, Sarı Üzüm, Kartal Çavuş and Razakı grape varieties grown in Bilecik province. int. j. agric. environ. food sci. 2024;8:729–735.
MLA
Özdemir Memiş, Seda, and Hayri Sağlam. “Shoot Tip Culture of Bilecik İrikarası, Sarı Üzüm, Kartal Çavuş and Razakı Grape Varieties Grown in Bilecik Province”. International Journal of Agriculture Environment and Food Sciences, vol. 8, no. 3, Sept. 2024, pp. 729-35, doi:10.31015/jaefs.2024.3.26.
Vancouver
1.Seda Özdemir Memiş, Hayri Sağlam. Shoot tip culture of Bilecik İrikarası, Sarı Üzüm, Kartal Çavuş and Razakı grape varieties grown in Bilecik province. int. j. agric. environ. food sci. 2024 Sep. 1;8(3):729-35. doi:10.31015/jaefs.2024.3.26