Occurrence of Antimicrobial Resistance in Salmonella spp. Isolated from Poultry Farms and Chicken Meat Preparations
Abstract
The aim of the study was to assess the prevalence, serotypes, and antimicrobial resistance profiles of Salmonella spp. isolated from poultry, poultry meat preparations in the Republic of Moldova, providing baseline data for future surveillance and control efforts. A total of 165 Salmonella isolates collected between January 2023 and April 2025 from layer farms, broiler farms, meat preparations and animal feed were identified, serotyped and tested for antimicrobial susceptibility using disc diffusion and broth microdilution methods, following EUCAST and EU regulatory standards. S. Enteritidis was the predominant serotype in layers, while S. Infantis was the most prevalent in broilers and meat preparations. Antimicrobial resistance was significantly higher in isolates from meat preparations compared to those from feces. High resistance to ciprofloxacin (up to 86.4%), nalidixic acid, tetracycline and sulfamethoxazole was observed, especially in isolates from meat preparations. Multidrug-resistant (MDR) strains were identified, with 10.4% of isolates from meat preparations and 3% from feces showing resistance to three or more antimicrobial classes. The study reveals concerning levels of antimicrobial resistance (AMR) to critically important antibiotics such as ciprofloxacin. These findings underscore the need for strengthened surveillance, regulation of antimicrobial use in agriculture and coordinated “One Health” strategies to mitigate AMR dissemination.
Keywords
Salmonella spp., Poultry, Serotyping, Antimicrobial resistance, One heal
References
- Abou Elez, R. M. M., Elsohaby, I., El-Gazzar, N., et al. (2021). Antimicrobial resistance of Salmonella enteritidis and Salmonella typhimurium from laying hens, table eggs and humans. Animals, 11(12), 3554. https://doi.org/10.3390/ani11123554
- Ball, T., Monte, D., Aidara-Kane, A., et al. (2020). International lineages of Salmonella enterica serovars isolated from chicken farms, Wakiso District, Uganda. PLoS ONE, 15(1), e0220484. https://doi.org/10.1371/journal.pone.0220484
- Balasubramanian, R., Im, J., Lee, J. S., et al. (2019). The global burden and epidemiology of invasive non-typhoidal Salmonella infections. Human Vaccines and Immunotherapeutics, 15(6), 1421–1426. https://doi.org/10.1080/21645515.2019.1581557
- Brenner, F. W., Villar, R. G., Angulo, F. J., Tauxe, R., & Swaminathan, B. (2000). Salmonella nomenclature. Journal of Clinical Microbiology, 38(7), 2465–2467. https://doi.org/10.1128/JCM.38.7.2465-2467.2000
- Castro-Vargas, R. E., Herrera-Sánchez, M. P., Rodríguez-Hernández, R., & Rondón-Barragán, I. S. (2020). Antibiotic resistance in Salmonella spp. isolated from poultry: A global overview. Veterinary World, 13(10), 2070–2084. https://doi.org/10.14202/vetworld.2020.2070-2084
- Chen, J., Huang, L., An, H., et al. (2024). One Health approach probes zoonotic non-typhoidal Salmonella in China: A systematic review. Journal of Global Health, 14, 04256. https://doi.org/10.7189/jogh.14.04256
- Cornelius, A. J., Carr, S. D., Bakker, S. N., Haysom, I. W., & Dyet, K. H. (2024). Antimicrobial resistance in selected bacteria from food animals in New Zealand 2018–2022. Journal of Food Protection, 87(4), 100245. https://doi.org/10.1016/j.jfp.2023.100245
- Dlamini, S. B., Mlambo, V., Mnisi, C. M., & Ateba, C. N. (2024). Virulence, MDR and biofilm formation in Salmonella species from poultry. PLoS ONE, 19(10), e0310010. https://doi.org/10.1371/journal.pone.0310010
- European Centre for Disease Prevention and Control. (2016). EU protocol for harmonised monitoring of antimicrobial resistance in human Salmonella and Campylobacter isolates. https://www.ecdc.europa.eu/en/publications-data/eu-protocol-harmonised-monitoring-antimicrobial-resistance-human-salmonella-and-0
- European Centre for Disease Prevention and Control, & European Food Safety Authority. (2024). The European Union summary report on antimicrobial resistance in zoonotic and indicator bacteria (2021–2022). EFSA Journal, 22(12), e08583. https://doi.org/10.2903/j.efsa.2024.8583
