Development and Evaluation of Functional Kefir-Enriched Ice Cream Development and Evaluation of Functional Ice Cream Enriched with Kefir

Year 2025, Volume: 9 Issue: 4, 1309 - 1315, 26.12.2025

Özge Köroğlu , Kenan Sinan Dayısoylu

https://doi.org/10.31015/2025.4.31

Abstract

This study aimed to produce ice cream with functional properties through the addition of kefir and to evaluate the resulting products in terms of chemical, physical, microbiological, and sensorial characteristics. Kefir, a fermented dairy product known for its complex microbial composition and potential health-promoting effects, was incorporated into ice cream to explore its functionality within a frozen matrix. The ice creams were prepared in two formulations: a kefir-free control and another containing 3% kefir. Both formulations were analyzed for physicochemical, microbiological, and sensory characteristics on days 0, 1, 15, and 30 of storage to monitor changes throughout the frozen shelf life. The findings indicated that kefir addition increased dry matter content (p < 0.01), viscosity (p < 0.01), and titratable acidity (p < 0.01), while significantly decreasing pH values (p < 0.01). These results reflect the impact of fermentation-derived metabolites on mix structure and acidity balance. Sensory evaluation revealed that the kefir-enriched group received significantly higher scores in texture and consistency (p < 0.01), as well as taste and aroma (p < 0.05), demonstrating improved consumer-relevant attributes compared to the control group. Microbiologically, a gradual decrease in the viability of Lactobacillus spp. and yeast-mold counts was observed during storage; however, a certain level of microbial viability was maintained throughout the study period, supporting the potential of ice cream as a carrier for probiotic or fermentation-derived functional components. In conclusion, kefir addition enhanced both the nutritional and sensory quality of ice cream, suggesting its potential as an effective strategy to promote kefir consumption and broaden consumer acceptance.

Keywords

Fermented dairy product , Functional food , Ice cream , Kefir , Probiotic

References

• Al, M., Zeren, F. E., Göçer, E. M. Ç., Demir, M., Küçükçetin, A. (2025). Effects of different processing methods on physicochemical and microbiological properties of kefir ice cream. International Dairy Journal, 160, 106111. https://doi.org/10.1016/j.idairyj.2024.106111
• Amiri, S., Kohneshahri, S. R. A., Nabizadeh, F. (2022). The effect of unit operation and adjunct probiotic culture on physicochemical, biochemical, and textural properties of Dutch Edam cheese. LWT, 155, 112859. https://doi.org/10.1016/j.lwt.2021.112859
• Arifan, F., Rizqiati, H., Hintono, A., Nurwantoro, N., Susanti, S., Sulistiyani, L. N. (2021). Effect of sugar substitution with dates puree (Phoenix dactylifera L.) on the physical and organoleptic characteristics of kefir ice cream. Jurnal Ilmu dan Teknologi Hasil Ternak, 16(1), 21–31. https://doi.org/10.21776/ub.jitek.2021.016.01.3
• Ayar, A., Sicramaz, H., Öztürk, S., Öztürk Yilmaz, S. (2018). Probiotic properties of ice creams produced with dietary fibres from by‐products of the food industry. International Journal of Dairy Technology, 71(1), 174–182. https://doi.org/10.1111/1471-0307.12387
• Avkan, F. (2020). Usage of pea protein isolate instead of milk powder in ice cream production. PhD Thesis, Harran University, Institute of Natural Sciences, Şanlıurfa.
• Azizi, N. F., Kumar, M. R., Yeap, S. K., Abdullah, J. O., Khalid, M., Omar, A. R., Osman, M. A., Mortadza, S. A. S., Alitheen, N. B. (2021). Kefir and its biological activities. Foods, 10(6), 1210. https://doi.org/10.3390/foods10061210
• Beegum, P. S., Nair, J. P., Manikantan, M. R., Pandiselvam, R., Shill, S., Neenu, S., Hebbar, K. B. (2022). Effect of coconut milk, tender coconut and coconut sugar on the physico-chemical and sensory attributes in ice cream. Journal of Food Science and Technology, 59(7), 2605–2616. https://doi.org/10.1007/s13197-021-05279-y
• Bengoa, A. A., Iraporda, C., Garrote, G. L., Abraham, A. G. (2019). Kefir micro‐organisms: their role in grain assembly and health properties of fermented milk. Journal of Applied Microbiology, 126(3), 686–700. https://doi.org/10.1111/jam.14107
• Butnariu, M., Sarac, I. (2019). Functional food. International Journal of Nutrition, 3(3), 7–16. https://doi.org/10.14302/issn.2379-7835.ijn-19-2615
• Cruz, A. G., Antunes, A. E., Sousa, A. L. O., Faria, J. A., Saad, S. M. (2009). Ice-cream as a probiotic food carrier. Food Research International, 42(9), 1233–1239. https://doi.org/10.1016/j.foodres.2009.03.020
• Çam, G., Akın, N., Konak Göktepe, Ç., Demirci, T. (2023). Pea (Pisum sativum L.) pod powder as a potential enhancer of probiotic Enterococcus faecium M74 in ice cream and its physicochemical, structural, and sensory effects. Journal of the Science of Food and Agriculture, 103(6), 3184–3193. https://doi.org/10.1002/jsfa.12276
• Dayısoylu, K. S., Gezginç, Y., & Cingöz, A. (2014). Fonksiyonel gıda mı, fonksiyonel bileşen mi? Gıdalarda fonksiyonellik. Gıda, 39(1), 57-62. http://dx.doi.org/10.5505/gida.03511 (in Turkish)
• Da Silva, P. D. L., de Fátima Bezerra, M., dos Santos, K. M. O., Correia, R. T. P. (2015). Potentially probiotic ice cream from goat's milk: Characterization and cell viability during processing, storage and simulated gastrointestinal conditions. LWT-Food Science and Technology, 62(1), 452–457. https://doi.org/10.1016/j.lwt.2014.02.055
• Demirci, M., & Gündüz, H. (1991). Süt teknoloğunun el kitabı. Hasad Yayıncılık. (in Turkish)
• Doğan, E. (2020). The utilization of whey powder in the production of ice cream. PhD Thesis, Ankara University, Institute of Natural Sciences, Ankara.
• Ebner, J., Arslan, A. A., Fedorova, M., Hoffmann, R., Küçükçetin, A., Pischetsrieder, M. (2015). Peptide profiling of bovine kefir reveals 236 unique peptides released from caseins during its production by starter culture or kefir grains. Journal of Proteomics, 117, 41–57. https://doi.org/10.1016/j.jprot.2015.01.005
• Guler-Akin, M. B., Goncu, B., Akin, M. S. (2016). Some properties of probiotic yoghurt ice cream supplemented with carob extract and whey powder. Advances in Microbiology, 6(14), 1010–1020. http://dx.doi.org/10.4236/aim.2016.614095
• Gürsoy, A., Türkmen, N. (2020). Süt ve süt ürünleri analiz yöntemleri. Dondurma analizleri. (Editör: Zübeyde Öner. Hatice Şanlıdere Aloğlu), Sidaş Medya Ltd. Şti., s.359-404. (in Turkish)
• Hagen, M., & Narvhus, J. A. (1999). Production of ice cream containing probiotic bacteria. Milchwissenschaft, 54(5), 265–268.
• Inoue, K., Shiota, K., Ito, T. (1998). Preparation and properties of ice cream type frozen yogurt. International Journal of Dairy Technology, 51(2), 44–50. https://doi.org/10.1111/j.1471-0307.1998.tb02506.x
• Kanca, N., Baş, B., Albayrak Delialioğlu, R., Gürsoy, A. (2023). Physical properties and bacterial viability of functional ice cream enriched with kefir. Veterinary Journal of Ankara University, 70(3). https://doi.org/10.33988/auvfd.1008552
• Kesenkas, H., Yerlikaya, O., Akpınar, A., Açu, M. (2013). Kefir dondurması üretiminde soya sütünün kullanım olanakları üzerine bir araştırma. Journal of Ege University Faculty of Agriculture (Türkiye), 50(1). (in Turkish)
• Kurt, A., Çakmakçı, S., & Çağlar, A. (1993). Süt ve Mamülleri Muayene ve Analiz Metotları Rehberi, 5 Baskı AÜ Yay No: 252/d Zir Fak Yay No: 18. Ders Kitapları Serisi No, 252. (in Turkish)
• Legassa, O. (2020). Ice cream nutrition and its health impacts. International Journal of Food and Nutritional Science, 7(1), 19–27. https://doi.org/10.15436/2377-0619.20.2678
• Onoruoiza, M. A., Ayodele, A. M., David-Momoh, T. E. (2024). A review on the effects of functional food on humans and microorganisms. International Journal of Probiotics and Dietetics, 4(1), 01–14.
• Özkaya, F., & Kuleaşan, H. (2000). Maya ve Küf “Gıda Mikrobiyolojisi ve Uygulamaları”, 2. Baskı, Sim Matbaacılık, Ankara. (in Turkish)
• Özkök, Ü. I. (1984). Herstellung von Kaschar-Käse mit Hilfe der Ultrafiltration und unter Einsatz von verschiedenen Kulturen mit und ohne Lipasezusatz (Doctoral dissertation, wydawca nieznany).
• Palamutoğlu, R. (2024). Fonksiyonel Probiyotik Gıdalar. Sağlık & Bilim (2024): Beslenme-II, 131. (in Turkish)
• Peate, I. (2021). The gastrointestinal system. British Journal of Healthcare Assistants, 15(3), 132–137. https://doi.org/10.12968/bjha.2021.15.3.132
• Pozhidaeva, E., Popov, E., Dymovskih, Y. (2019). Exopolysaccharide fermented milk ice cream. In 1st International Symposium Innovations in Life Sciences (ISILS 2019) (pp. 352–355). Atlantis Press. https://doi.org/10.2991/isils-19.2019.61
• Rolon, M. L., Bakke, A. J., Coupland, J. N., Hayes, J. E., Roberts, R. F. (2017). Effect of fat content on the physical properties and consumer acceptability of vanilla ice cream. Journal of Dairy Science, 100(7), 5217–5227. https://doi.org/10.3168/jds.2016-12379
• Salem, M. M., Fathi, F. A., Awad, R. A. (2005). Production of probiotic ice cream. Polish Journal of Food and Nutrition Sciences, 55(3), 267–271.
• Sedefoğlu, S., Ortakcı, F., Sert, S. (2022). Enkapsüle edilmiş ve serbest formda probiyotik Lactobacillus acidophilus ATCC 4356 suşunun dondurma depolama periyodunda stabilitesinin incelenmesi. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 53(1), 14–23. https://doi.org/10.17097/ataunizfd.913445 (in Turkish)
• Shazly, A. B., Fouad, M. T., Elaaser, M., Sayed, R. S., Abd El‐Aziz, M. (2022). Probiotic coffee ice cream as an innovative functional dairy food. Journal of Food Processing and Preservation, 46(12), e17253. https://doi.org/10.1111/jfpp.17253
• Süren, B. N., Salman, S., Kaya, E., Büyükkal, Y., Kutlu, G., Törnük, F. (2024). Symbiotic ice-cream production using Lactiplantibacillus plantarum and oleaster (Elaeagnus angustifolia L.) flour. Harran Tarım ve Gıda Bilimleri Dergisi, 28(3), 444–458. https://doi.org/10.29050/harranziraat.1484737
• Toommuangpak, W., Thaiudom, S. (2024). Yogurt ice cream with Bifidobacterium longum subsp. longum TISTR 2195: Effects of fat content and prebiotics on physicochemical and rheological properties, ice cream microstructure, and probiotic survival. International Journal of Food Properties, 27(1), 641–656. https://doi.org/10.1080/10942912.2024.2344565
• Turgut, T., Cakmakci, S. (2009). Investigation of the possible use of probiotics in ice cream manufacture. International Journal of Dairy Technology, 62(3), 444–451. https://doi.org/10.1111/j.1471-0307.2009.00494.x