Influence of migratory and stationary beekeeping on honey bee wing morphology and propolis chemical composition

Özge Tozkar; Cengiz Erkan

doi:10.31015/jaefs.2026.1.28

Influence of migratory and stationary beekeeping on honey bee wing morphology and propolis chemical composition

Abstract

This study examines the effects of migratory and stationary beekeeping practices on the chemical composition of propolis and the morphological variations in honeybees from Eastern Anatolia region of Turkey. The chemical composition of propolis (21 samples) was examined through HPLC, focusing on phenolic compounds, total tannin content, and FRAP antioxidant values. To assess honeybee morphology, geometric morphometric analysis of wings was conducted on 564 worker bee samples from 18 hives across 4 locations, revealing significant differences between migratory and stationary colonies (p < 0.0001). Honeybees from migratory colonies exhibited greater morphological diversity, likely reflecting exposure to a wider range of environmental conditions. The key phenolic compounds identified included caffeic acid, resveratrol, and quercetin. Migratory beekeeping resulted in significantly higher levels of caffeic acid, chrysin, and resveratrol (p ≤ 0.01) in propolis, while stationary beekeeping produced products with stronger antioxidant capacities, as indicated by higher FRAP values (2410.44 vs 2056.48 µmol TE/100 g, p < 0.05). Correlation analyses between phenolic compounds revealed strong positive relationships (r = 0.63–0.97, p < 0.01), indicating synergistic interactions that enhance the bioactivity of propolis. The results suggest that migratory beekeeping enhances the chemical richness of propolis, while stationary beekeeping promotes stronger antioxidant capacity, offering different benefits depending on the intended use of propolis. These findings indicate that beekeeping management practices can directly influence both propolis phenolic profiles and honey bee wing morphometry. Specifically, migratory practices should be adopted for propolis with enhanced bioactive compound concentrations, while stationary methods are preferable for maximizing antioxidant potential in pharmaceutical formulations.

Keywords

FRAP assay, HPLC, Migratory beekeeping, Phenolic compounds, Stationary beekeeping, Tannin content

Supporting Institution

The authors are grateful to the Scientific Research Projects Department of Van Yüzüncü Yıl University (project no: FHD-2022-10107) for financial support.

Project Number

FHD-2022-10107

References

Ahn, M.R., S. Kumazawa, Y. Usui, J. Nakamura, M. Matsuka, F. Zhu and T. Nakayama (2007). Antioxidant activity and constituents of propolis collected in various areas of China. Food Chem. 101(4): 1383-1392. https://doi.org/10.1016/j.foodchem.2006.03.045
Aliyazıcıoğlu, R., H. Şahin, O. Ertürk, E. Ulusoy and S. Kolaylı (2013). Properties of phenolic composition and biological activity of propolis from Turkey. Int. J. Food Prop. 16: 277-287. https://doi.org/10.1080/10942912.2010.551312
Alvear, M., E. Santos, F. Cabezas, A. Pérez-SanMartín, M. Lespinasse and J. Veloz (2021). Geographic area of collection determines the chemical composition and antimicrobial potential of three extracts of Chilean propolis. Plants 10(8): 1543. https://doi.org/10.3390/plants10081543
Bayram, N., K. Sorkun, G. Öz, B. Salih and G. Topcu (2018). Chemical characterization of 64 propolis samples from Hakkari, Turkey. Rec. Nat. Prod. 12(6): 569-581. https://doi.org/10.25135/rnp.78.16.12.585
Bhargava, P., D. Mahanta, A. Kaul, Y. Ishida, K. Terao, R. Wadhwa and S. Kaul (2021). Experimental evidence for therapeutic potentials of propolis. Nutrients 13: 2528. https://doi.org/10.3390/nu13082528
Bookstein, F.L. (1991). Morphometric Tools for Landmark Data: Geometry and Biology. Cambridge University Press; Cambridge, UK. 435 p. https://doi.org/10.1017/CBO9780511573064
Bouga, M., C. Alaux, M. Bienkowska, R. Büchler, N.L. Carreck, E. Cauia, R. Chlebo, B. Dahle, R. Dall'Olio, P. de La Rúa, A. Gregorc, E. Ivanova, A. Kence, M. Kence, N. Kezić, H. Kiprijanovska, P. Kozmus, P. Kryge, Y. Le Conte, M. Lodesani, A.M. Murilhas, A. Siceanu, G. Soland, A. Uzunov and J. Wilde (2011). A review of methods for discrimination of honey bee populations as applied to European beekeeping. J. Apicult. Res. 50(1): 51-84. https://doi.org/10.3896/IBRA.1.50.1.06
Can, Z., Y. Kara, S. Kolaylı and İ. Çakmak (2024). Antioxidant activity and phenolic composition of propolis from Marmara Region, Turkey. J. Apicult. Res. 63(3), 542–548. https://doi.org/10.1080/00218839.2022.2157582
Can, Z., O. Yıldız, H. Şahin, A. Asadov and S. Kolaylı (2015). Phenolic profile and antioxidant potential of propolis from Azerbaijan. Mellifera 15(1): 16-28. Retrieved from https://dergipark.org.tr/tr/pub/mellifera/issue/17834/186990 (Accessed: 14 February 2026).
Christov, R., B. Trusheva, M. Popova, V. Bankova and M. Bertrand (2006). Chemical composition of propolis from Canada, its antiradical activity and plant origin. Nat. Prod. Res. 20(6): 531-536. https://doi.org/10.1080/14786410512331328159

Cirasino, L., A. Pisati and F. Fasani (1987). Contact dermatitis from propolis. Contact Dermatitis 16: 110-111. https://doi.org/10.1111/j.1600-0536.1987.tb01394.x
Çelemli, Ö.G. (2015). Chemical classification of propolis samples collected from different regions of Turkey in geographical region base. Hacettepe J. Biol. Chem. 43(1): 49-57. Retrieved from https://dergipark.org.tr/en/pub/hjbc/issue/61895/926205 (Accessed: 14 February 2026).
Davis, P.H. (1965-2000). Flora of Turkey and East Aegean Islands. University Press; Edinburgh, UK. Retrieved from https://www.jstor.org/stable/10.3366/j.ctvxcrdfg (Accessed: 14 February 2026).
FAO (Food and Agriculture Organization of the United Nations). (2024). Leading producers of natural honey worldwide in 2022. FAOSTAT Database. FAO; Rome (Italy). Retrieved from https://www.fao.org/faostat/en/#data (Accessed: 14 February 2026).
Fujimoto, T., J. Nakamura and M. Matsuka (2001). Diversity of propolis, Part 1. Propolis from the world. Honeybee Sci. 22: 9-16. Retrieved from https://www.researchgate.net/publication/292023892_Diversity_of_propolis_Part_1_Propolis_from_the_world (Accessed: 14 February 2026).
Gençay, Ö. and B. Salih (2009). GC-MS analysis of propolis samples from 17 different regions of Turkey, four different regions of Brazil and one from Japan. Mellifera 9: 19-28.
Ghisalberti, E.L. (1979). Propolis: A review. Bee World 60(2): 59-84. https://doi.org/10.1080/0005772X.1979.11097738
Greenaway, W., J. May, T. Scaysbrook and F. Whatley (1991). Identification by gas chromatography-mass spectrometry of 150 compounds in propolis. Z. Naturforsch. 46c: 111-121. https://doi.org/10.1515/znc-1991-1-218
Gülçin, I., E. Bursal, M.H. Şehitoğlu, M. Bilsel and A.C. Gören (2010). Polyphenol contents and antioxidant activity of lyophilized aqueous extract of propolis from Erzurum, Turkey. Food Chem. Toxicol. 48(8-9): 2227-2238. https://doi.org/10.1016/j.fct.2010.05.053
Herrera-López, M., P. Richomme, L. Peña-Rodríguez and L. Calvo-Irabién (2023). Bee species, botanical sources and the chemical composition of propolis from Yucatan, Mexico. J. Chem. Ecol. 49: 1-10. https://doi.org/10.1007/s10886-023-01429-y
Işıldak, Ö., İ. Yıldız, N. Genç, D. Sabancı and İ. Işıldak (2023). New potentiometric PVC membrane electrode for Ferric Reduction Antioxidant Power assay. Food Chem. 423: 136261. https://doi.org/10.1016/j.foodchem.2023.136261
Klingenberg, C.P. (2011). MorphoJ: An integrated software package for geometric morphometrics. Mol. Ecol. Resour. 11(2): 353-357. https://doi.org/10.1111/j.1755-0998.2010.02924.x
Koru, O., F. Toksoy, C.H. Acikel, Y.M. Tunca, M. Baysallar, A. Uskudar Guclu, E. Akca, A. Ozkok Tuylu, K. Sorkun, M. Tanyuksel and B. Salih (2007). In vitro antimicrobial activity of propolis samples from different geographical origins against certain oral pathogens. Anaerobe 13(3-4): 140-145. https://doi.org/10.1016/j.anaerobe.2007.02.001
Marcucci, M.C. (1995). Propolis: chemical composition, biological properties and therapeutic activity. Apidologie 26: 83-99. https://doi.org/10.1051/apido:19950202
Markham, K.R., K.A. Mitchell, A.L. Wilkins, J.A. Daldy and Y. Lu (1996). HPLC and GC-MS identification of the major organic constituents in New Zealand propolis. Phytochemistry 42: 205-211. https://doi.org/10.1016/0031-9422(96)83286-9
Mayworm, M.A.S., C.A. Lima, A.C.B. Tomba, C.C. Fernandes-Silva, M.L.F. Salatino and A. Salatino (2014). Does propolis contain tannins? Complement. Altern. Med. 2014: 613647. https://doi.org/10.1155/2014/613647
Mercan-Dogan, N., İ. Kivrak, M. Duru, H. Katırcıoğlu, S. Gülcan, S. Malcı, G. Acar and B. Salih (2006). Chemical composition effects onto antimicrobial and antioxidant activities of propolis collected from different regions of Turkey. Ann. Microbiol. 56: 373-378. https://doi.org/10.1007/BF03175035
Mohammadzadeh, S., M. Shariatpanahi, M. Hamedi, R. Ahmadkhaniha, N. Samadi and S.N. Ostad (2007). Chemical composition, oral toxicity and antimicrobial activity of Iranian propolis. Food Chem. 103(4): 1097-1103. https://doi.org/10.1016/j.foodchem.2006.10.006
Moreira, L., L.G. Dias, J.A. Pereira and L. Estevinho (2008). Antioxidant properties, total phenols and pollen analysis of propolis samples from Portugal. Food Chem. Toxicol. 46: 3482-3485. https://doi.org/10.1016/j.fct.2008.08.025
Oruc, H., M. Cayci, A. Sorucu, E. Uzabacı and N. Ramadhan (2023). Characterization of commercially available propolis products in Turkey based on individual phenolic compounds. J. Apicult. Res. 62(5), 1225–1232. https://doi.org/10.1080/00218839.2021.1962110
Öztürk, N., M. Tunçel and N.B. Tunçel (2007). Determination of phenolic acids by a modified HPLC: Its application to various plant materials. J. Liq. Chromatogr. Relat. Technol. 30: 587-596. https://doi.org/10.1080/10826070601093911
Palmer, M.R., D.R. Smith and O. Kaftanoğlu (2000). Turkish honeybees: Genetic variation and evidence for a fourth lineage of Apis mellifera mtDNA. J. Hered. 91: 42-46. https://doi.org/10.1093/jhered/91.1.42
Rohlf, F.J. (2000). Statistical power comparisons among alternative morphometric methods. Am. J. Phys. Anthropol. 111: 463-478.
Rohlf, F.J. (2018). TpsDig Version 2.31. Ecology & Evolution, SUNY at Stony Brook; USA.
Rohlf, F.J. (2019). TpsUtil32 v.1.78 software: tpsUtil (Version 1.78). SUNY Stony Brook; USA.
Ruttner, F. (1988). Morphometric analysis and classification. Biogeography and Taxonomy of Honey Bees 66-78. https://doi.org/10.1007/978-3-642-72649-1_6
Salomão, K., A.P. Dantas, C.M. Borba, L.C. Campos, D.G. Machado, F.R. Aquino Neto and S.L. de Castro (2004). Chemical composition and microbicidal activity of extracts from Brazilian and Bulgarian propolis. Lett. Appl. Microbiol. 38(2): 87-92. https://doi.org/10.1111/j.1472-765x.2003.01458.x
Saral, O., M. Kılıçarslan, H. Şahin, O. Yıldız and B. Dinçer (2019). Evaluation of antioxidant activity of bee products of different bee races in Turkey. Turk. J. Vet. Anim. Sci. 43: 441-447. https://doi.org/10.3906/vet-1901-3
Sarıkaya, A., E. Ulusoy, N. Öztürk, M. Tunçel and S. Kolaylı (2009). Antioxidant activity and phenolic acid constituents of chestnut (Castanea sativa Mill.) honey and propolis. J. Food Biochem. 33: 470-481. https://doi.org/10.1111/j.1745-4514.2009.00231.x
Schnitzler, P., A. Neuner, S. Nolkemper, C. Zundel, H. Nowack, K.H. Sensch and J. Reichling (2010). Antiviral activity and mode of action of propolis extracts and selected compounds. Phytother. Res. 24: S20-S28. https://doi.org/10.1002/ptr.2868
Sulaiman, G., K. Sammarrae, M. Zucchetti, R. Frapolli, E. Bello, E. Erba, M. D'Incalci and R. Bagnati (2011). Chemical characterization of Iraqi propolis samples and assessing their antioxidant potentials. Food Chem. Toxicol. 49: 2415-2421. https://doi.org/10.1016/j.fct.2011.06.060
Takaisi-Kikuni, N.B. and H. Schilcher (1994). Electron microscopic and microcalorimetric investigations of the possible mechanism of the antibacterial action of a defined propolis provenance. Planta Med. 60: 222-227. https://doi.org/10.1055/s-2006-959463
Tapia, A., J. Rodriguez, C. Theoduloz, S. Lopez, G.E. Feresin and G. Schmeda-Hirschmann (2004). Free radical scavengers and antioxidants from Baccharis grisebachii. J. Ethnopharmacol. 95(2-3): 155-161. https://doi.org/10.1016/j.jep.2004.06.035
Uzel, A., K. Sorkun, O. Onçağ, D. Coğulu, O. Gençay and B. Salih (2005). Chemical compositions and antimicrobial activities of four different Anatolian propolis samples. Microbiol. Res. 160(2): 189-195. https://doi.org/10.1016/j.micres.2005.01.002
Velazquez, C., M. Navarro, A. Acosta, A. Angulo, Z. Dominguez, R. Robles, R. Robles-Zepeda, E. Lugo, F.M. Goycoolea, E.F. Velazquez, H. Astiazaran and J. Hernandez (2007). Antibacterial and free-radical scavenging activities of Sonoran propolis. Appl. Microbiol. 103: 1747-1756. https://doi.org/10.1111/j.1365-2672.2007.03409.x
Woźniak, M., A. Sip, L. Mrówczyńska, J. Broniarczyk, A. Waśkiewicz and I. Ratajczak (2023). Biological activity and chemical composition of propolis from various regions of Poland. Molecules 28(1): 141. https://doi.org/10.3390/molecules28010141

Details

Primary Language

English

Subjects

Zootechny (Other), Agricultural Biotechnology (Other)

Journal Section

Research Article

Authors

Özge Tozkar ^*
0000-0003-2135-955X
Türkiye

Cengiz Erkan
0000-0003-3510-2800
Türkiye

Early Pub Date

March 21, 2026

Publication Date

March 21, 2026

Submission Date

February 11, 2026

Acceptance Date

March 16, 2026

Published in Issue

Year 2026 Volume: 10 Number: 1

DOI

https://doi.org/10.31015/jaefs.2026.1.28

IZ

https://izlik.org/JA35GF27GU

APA

Tozkar, Ö., & Erkan, C. (2026). Influence of migratory and stationary beekeeping on honey bee wing morphology and propolis chemical composition. International Journal of Agriculture Environment and Food Sciences, 10(1), 258-271. https://doi.org/10.31015/jaefs.2026.1.28

AMA

1.Tozkar Ö, Erkan C. Influence of migratory and stationary beekeeping on honey bee wing morphology and propolis chemical composition. int. j. agric. environ. food sci. 2026;10(1):258-271. doi:10.31015/jaefs.2026.1.28

Chicago

Tozkar, Özge, and Cengiz Erkan. 2026. “Influence of Migratory and Stationary Beekeeping on Honey Bee Wing Morphology and Propolis Chemical Composition”. International Journal of Agriculture Environment and Food Sciences 10 (1): 258-71. https://doi.org/10.31015/jaefs.2026.1.28.

EndNote

Tozkar Ö, Erkan C (March 1, 2026) Influence of migratory and stationary beekeeping on honey bee wing morphology and propolis chemical composition. International Journal of Agriculture Environment and Food Sciences 10 1 258–271.

IEEE

[1]Ö. Tozkar and C. Erkan, “Influence of migratory and stationary beekeeping on honey bee wing morphology and propolis chemical composition”, int. j. agric. environ. food sci., vol. 10, no. 1, pp. 258–271, Mar. 2026, doi: 10.31015/jaefs.2026.1.28.

ISNAD

Tozkar, Özge - Erkan, Cengiz. “Influence of Migratory and Stationary Beekeeping on Honey Bee Wing Morphology and Propolis Chemical Composition”. International Journal of Agriculture Environment and Food Sciences 10/1 (March 1, 2026): 258-271. https://doi.org/10.31015/jaefs.2026.1.28.

JAMA

1.Tozkar Ö, Erkan C. Influence of migratory and stationary beekeeping on honey bee wing morphology and propolis chemical composition. int. j. agric. environ. food sci. 2026;10:258–271.

MLA

Tozkar, Özge, and Cengiz Erkan. “Influence of Migratory and Stationary Beekeeping on Honey Bee Wing Morphology and Propolis Chemical Composition”. International Journal of Agriculture Environment and Food Sciences, vol. 10, no. 1, Mar. 2026, pp. 258-71, doi:10.31015/jaefs.2026.1.28.

Vancouver

1.Özge Tozkar, Cengiz Erkan. Influence of migratory and stationary beekeeping on honey bee wing morphology and propolis chemical composition. int. j. agric. environ. food sci. 2026 Mar. 1;10(1):258-71. doi:10.31015/jaefs.2026.1.28

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Influence of migratory and stationary beekeeping on honey bee wing morphology and propolis chemical composition

Influence of migratory and stationary beekeeping on honey bee wing morphology and propolis chemical composition

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