Investigation of biological activities and bioactive compounds of Rhynchostegium megapolitanum collected from Aydın, Türkiye

Birsen Kırım; Rukiye Yavaşer Boncooğlu; Mehran Aksel; Ali Özmen; Esin Poyrazoğlu; Safiye Emirdağ; Hacı Halil Bıyık; A. Alev Karagözler; İlke Demirkaya; İlknur Babahan Bırcan

doi:10.31015/jaefs.2026.3.5

Investigation of biological activities and bioactive compounds of Rhynchostegium megapolitanum collected from Aydın, Türkiye

Abstract

This study examines the antioxidant, antimicrobial, and anticancer properties of methanol, chloroform, and n-hexane extracts from Rhynchostegium megapolitanum, a moss species collected in Aydın, Türkiye. Total phenolic, flavonoid, and tannin contents were quantified. Antioxidant activity was evaluated using DPPH and ABTS assays, yielding 76.0%, 71.4% and 7.93% DPPH and 59.1%, 21.3% and 4.44% ABTS radical scavenging activities for methanol, chloroform and hexane extracts, respectively. Antimicrobial activity was tested against 17 bacteria and 4 yeasts, showing significant efficacy, particularly against Gram-positive bacteria. Methanol extracts of R. megapolitanum exhibited notable antibacterial effects. Anticancer activity was evaluated on PC-3 and MCF-7 cancer cell lines. Cytotoxicity was assessed using Hoechst/PI double staining, and paclitaxel (1 µM) served as a positive control. The extracts, in apoptosis mechanism demonstrated concentration-dependent antiproliferative effects (5–40 µM), supporting their potential as natural therapeutic agents in cancer research.

Keywords

Bryophytes, Rhynchostegium megapolitanum, Antioxidant, Antimicrobial, Anticancer

Supporting Institution

No funding.

Thanks

The authors are grateful to Prof. Dr. Adnan Erdag, from biology department (Aydin Adnan Menderes University, Faculty of Sciences, Aydin, Republic of Türkiye) for Rhynchostegium megapolitanum identification.

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Details

Primary Language

English

Subjects

Aquaculture and Fisheries (Other)

Journal Section

Research Article

Authors

Birsen Kırım ^*
0000-0003-3292-1368
Türkiye

Rukiye Yavaşer Boncooğlu
0000-0003-2295-4617
Türkiye

Mehran Aksel
0000-0002-3942-2257
Türkiye

Ali Özmen
0000-0003-4364-4853
Türkiye

Esin Poyrazoğlu
0000-0002-3921-5362
Türkiye

Safiye Emirdağ
0000-0002-1676-2881
Türkiye

Hacı Halil Bıyık
0000-0003-0258-054X
Türkiye

A. Alev Karagözler
0000-0002-1884-0089
Türkiye

İlke Demirkaya
0000-0002-9402-6724
Türkiye

İlknur Babahan Bırcan
0000-0002-1336-671X
Türkiye

Early Pub Date

August 29, 2026

Publication Date

-

Submission Date

June 5, 2026

Acceptance Date

August 15, 2026

Published in Issue

Year 2026 Number: Advanced Online Publication

DOI

https://doi.org/10.31015/jaefs.2026.3.5

IZ

https://izlik.org/JA49HP62PK

APA

Kırım, B., Yavaşer Boncooğlu, R., Aksel, M., Özmen, A., Poyrazoğlu, E., Emirdağ, S., Bıyık, H. H., Karagözler, A. A., Demirkaya, İ., & Babahan Bırcan, İ. (2026). Investigation of biological activities and bioactive compounds of Rhynchostegium megapolitanum collected from Aydın, Türkiye. International Journal of Agriculture Environment and Food Sciences, Advanced Online Publication, 579-595. https://doi.org/10.31015/jaefs.2026.3.5

AMA

1.Kırım B, Yavaşer Boncooğlu R, Aksel M, et al. Investigation of biological activities and bioactive compounds of Rhynchostegium megapolitanum collected from Aydın, Türkiye. int. j. agric. environ. food sci. 2026;(Advanced Online Publication):579-595. doi:10.31015/jaefs.2026.3.5

Chicago

Kırım, Birsen, Rukiye Yavaşer Boncooğlu, Mehran Aksel, et al. 2026. “Investigation of Biological Activities and Bioactive Compounds of Rhynchostegium Megapolitanum Collected from Aydın, Türkiye”. International Journal of Agriculture Environment and Food Sciences, no. Advanced Online Publication: 579-95. https://doi.org/10.31015/jaefs.2026.3.5.

EndNote

Kırım B, Yavaşer Boncooğlu R, Aksel M, Özmen A, Poyrazoğlu E, Emirdağ S, Bıyık HH, Karagözler AA, Demirkaya İ, Babahan Bırcan İ (August 1, 2026) Investigation of biological activities and bioactive compounds of Rhynchostegium megapolitanum collected from Aydın, Türkiye. International Journal of Agriculture Environment and Food Sciences Advanced Online Publication 579–595.

IEEE

[1]B. Kırım et al., “Investigation of biological activities and bioactive compounds of Rhynchostegium megapolitanum collected from Aydın, Türkiye”, int. j. agric. environ. food sci., no. Advanced Online Publication, pp. 579–595, Aug. 2026, doi: 10.31015/jaefs.2026.3.5.

ISNAD

Kırım, Birsen - Yavaşer Boncooğlu, Rukiye - Aksel, Mehran - Özmen, Ali - Poyrazoğlu, Esin - Emirdağ, Safiye - Bıyık, Hacı Halil - Karagözler, A. Alev - Demirkaya, İlke - Babahan Bırcan, İlknur. “Investigation of Biological Activities and Bioactive Compounds of Rhynchostegium Megapolitanum Collected from Aydın, Türkiye”. International Journal of Agriculture Environment and Food Sciences. Advanced Online Publication (August 1, 2026): 579-595. https://doi.org/10.31015/jaefs.2026.3.5.

JAMA

1.Kırım B, Yavaşer Boncooğlu R, Aksel M, Özmen A, Poyrazoğlu E, Emirdağ S, Bıyık HH, Karagözler AA, Demirkaya İ, Babahan Bırcan İ. Investigation of biological activities and bioactive compounds of Rhynchostegium megapolitanum collected from Aydın, Türkiye. int. j. agric. environ. food sci. 2026;:579–595.

MLA

Kırım, Birsen, et al. “Investigation of Biological Activities and Bioactive Compounds of Rhynchostegium Megapolitanum Collected from Aydın, Türkiye”. International Journal of Agriculture Environment and Food Sciences, no. Advanced Online Publication, Aug. 2026, pp. 579-95, doi:10.31015/jaefs.2026.3.5.

Vancouver

1.Birsen Kırım, Rukiye Yavaşer Boncooğlu, Mehran Aksel, Ali Özmen, Esin Poyrazoğlu, Safiye Emirdağ, Hacı Halil Bıyık, A. Alev Karagözler, İlke Demirkaya, İlknur Babahan Bırcan. Investigation of biological activities and bioactive compounds of Rhynchostegium megapolitanum collected from Aydın, Türkiye. int. j. agric. environ. food sci. 2026 Aug. 1;(Advanced Online Publication):579-95. doi:10.31015/jaefs.2026.3.5

International Journal of Agriculture, Environment and Food Sciences

e-ISSN: 2618-5946 | Publisher: Gültekin Özdemir | DOI Prefix: 10.31015

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