Effect of exogenous proline application on yield and yield components of second-crop maize under water deficit conditions
Abstract
Water scarcity is a major constraint limiting maize productivity in arid and semi-arid regions. This study evaluated the effects of exogenous proline application on yield, yield components, and water productivity of second-crop maize under different irrigation levels. The field experiment was conducted at Harran University, Şanlıurfa, Türkiye, using a split-plot design in randomized complete blocks with three replications. Irrigation levels consisted of 100% (IW1), 75% (IW2), and 50% (IW3) of full irrigation, while proline (Pro) treatments included a control and 1 mM exogenous proline. Increasing water deficit reduced plant height, cob length, cob diameter, thousand-grain weight, hectoliter weight, and grain yield. However, proline application improved most traits, particularly under deficit irrigation. Compared with the control, proline increased grain yield by 3.53%, 41.05%, and 43.98% under IW1, IW2, and IW3, respectively. Seasonal irrigation water ranged from 392 to 723 mm, while seasonal crop evapotranspiration varied between 411 and 731 mm. The highest irrigation water productivity (1.77 kg m⁻³) and water productivity (1.70 kg m⁻³) were obtained from the Pro-IW2 treatment. Principal component analysis showed a clear separation of treatments according to irrigation level and proline application. As a result, 1 mM exogenous proline application may be a promising approach for improving yield performance and water productivity of second-crop maize under semi-arid conditions.
Keywords
Deficit Irrigation, Exogenous Proline, Maize (Zea mays L.), Yield Components, Water Stress
Supporting Institution
Project Number
Ethical Statement
Thanks
References
- Abebaw, S. E. (2025). A global review of the impacts of climate change and variability on agricultural productivity and farmers' adaptation strategies. Food Science & Nutrition, 13(5), e70260. https://doi.org/10.1002/fsn3.70260
- Ağaçkesen, M. N., & Öktem, A. (2020). Farklı zamanlarda yapılan hasadın Merit tatlı mısır çeşidinde (Zea mays L. Saccharata Sturt) taze koçan verimi ve bazı verim unsurlarına etkisi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 23(1), 69–76. https://doi.org/10.18016/ksutarimdoga.vi.610712
- Akçalı, C. T., & Gözübenli, H. (2020). Effects of Different Irrigation Intervals on Yield Parameters and Popping Quality of Popcorn (Zea Mays Everta Sturt.) Cultivated in Amik Plain as Second Crop. KSU J. Agric Nat ,23 (5): 1184-1191.
- Akdeniz, H., Keskin, B., & Yılmaz, İ. H. (2016). Effects of deficit irrigation on yield and yield components of grain corn (Zea mais L.). In D. Kovačević (Ed.), Proceedings of the VII International Scientific Agriculture Symposium "Agrosym 2016" (pp. 602–611). University of East Sarajevo.
- Akın, S., Şimşek, M., Sarıoğlu, A., & Keskiner, A. D. (2020). Mikoriza uygulaması ve farklı sulama seviyelerinin geç dönemde yetiştirilen hıyarın verim ve verim bileşenleri üzerine etkisi. Harran Tarım ve Gıda Bilimleri Dergisi, 24(2), 241–249. https://doi.org/10.29050/harranziraat.660670
- Akram, S., Kibria, M. G., Murata, Y., & Hoque, M. A. (2021). Exogenously applied proline modulates drought tolerance in maize (Zea Mays L). International Journal of Plant & Soil Science, 33(19), 198–212.
- Ali, Q., Anwar, F., Ashraf, M., Saari, N., & Perveen, R. (2013). Ameliorating effects of exogenously applied proline on seed composition, seed oil quality and oil antioxidant activity of maize (Zea mays L.) under drought stress. International Journal of Molecular Sciences, 14(1), 818–835.
- Alkhafagi, H. H. A., Al-Janabi, Y. A., & Al-Khafagi, K. F. H. (2025). Effect of exogenous proline on drought stress tolerance in wheat (Triticum aestivum L.) cultivars. Plant Science Today, 12(4). https://doi.org/10.14719/pst.9266
- Bölük, E. (2016). Aydeniz iklim sınıflandırmasına göre Türkiye iklimi. Meteoroloji Genel Müdürlüğü. Ankara.
- Çakır, R. (2004). Effect of water stress at different development stages on vegetative and reproductive growth of corn. Field Crops Research, 89(1), 1–16.


