Interaction between culture filtrates of Fusarium culmorum isolates and some root lesion nematodes

Year 2021, Volume: 5 Issue: 1, 85 - 91, 31.03.2021

Fatma Gül Göze Özdemir , Bülent Yaşar , Şerife Evrim Arıcı

https://doi.org/10.31015/jaefs.2021.1.12

Cited By: 1

https://izlik.org/JA62WX45XK

Abstract

This study was conducted with root lesion nematodes Pratylenchus penetrans, P. thornei and P. neglectus and three isolates of Fusarium culmorum culture filtrates (ISP, B4, FC5) in vitro conditions. The culture filtrates were diluted 1/0,1/10, 1/20, 1/30, 1/40, 1/50, 1/60, 1/70, 1/80, 90, 1/100 in 1.5 ml microtubes, 250 µl of root lesion nematodes adult+larvae were placed in each microtubes that containing different concentrations of culture filtrate with 50 μl of purified water together with micropipette and incubated at 25±1°C. Nematode mortality were determined after 24 h, 48 h and 72 h. As a result, The mortality effect of culture filtrate of FC5 isolates was determined to be high on three lesion nematode species. Fusarium culmorum culture filtrates of B4 and ISP isolates caused more deaths on P. penetrans than P.thornei and P. neglectus. It has been found to mortality rate increase over time in vitro. The lowest mortality rates were generally found at concentrations of culture filtrates of 1/100 and 1/90, while the highest mortality rates were found at concentrations of 1/0 and 1/10. No difference on P. thornei mortality was detected between the three isolates of F. culmorum culture filtrate at each diluted concentration. In the study, the antagonistic relationship between culture filtrates from F. culmorum isolates and root lesion nematodes were determined in vitro conditions.

Keywords

Antagonism , Culture filtrate , Fusarium culmorum , Root lesion nematodes , Interaction

Supporting Institution

Isparta Uygulamalı Bilimler Üniversitesi ÖYP Kurum Kordinatörlüğü

Project Number

ÖYP05551-DR-14

Thanks

Acknowledgements We thank to Isparta University of Applied Sciences Teaching Staff Training Program should be greatly appreciated for the contributions of providing financial support for the project “ OYP05551-DR-14”

References

• Arıcı, Ş. E., Arap, Ü. and Yatağan, F. B. (2013). Identification of Soilborne Fungal Dieases on Wheat Field in Province of Isparta and Burdur. Suleyman Demirel University, Journal of Natural and Applied Science, 17(2), 26-30.
• Arıcı, Ş. E. (2006). In Vitro Selection for Resıstans to Head Blight (Fusarium Spp.) Vıa Somaclonal Variatıon in Wheat (Triticum aestivum L.) Phd Thesıs, University of Çukurova Institute Of Natural and Applied Sciences Department of Plant, Adana.
• Athman, S. Y., Dubois, T., Viljoen, A., Labuschagne, N., Coyne, D., Ragama, P., Gold, C. and Niere, B. (2006). In vitro antagonism of endophytic Fusarium oxysporum isolates against the burrowing nematode Radopholus similis. Journal of Nematology, 8(4), 627–636.
• Back, M. A., Haydock, P. P. J. and Jenkinson, P. (2002). Disease complexes involving plant parasitic nematodes and soilborne pathogens. Plant pathology, 51(6), 683-697.
• Badea, E., Sandulescu, D., Gozia, O., Radutoıu, E., Raicu, P. and Gorenflot, R. (1997). Effets du filtrat de culture de Fusarium graminearum et de la température sur l’embryogenèse somatique chez Triticum aestivum L. Revue de cytologie et de biologie végétales, Le Botaniste, 20(3-4), 3-10.
• Bankole, S. A. and Adebanjo, A. (2003). Mycotoxins in food in West Africa: current situation and possibilities of controlling it. African Journal of Biotechnology, 2(9), 254-263.
• Bhagawati, B., Goswami, B. K., and Singh, C. S. (2000). Management of disease complex of tomato caused by Meloidogyne incognita and Fusarium oxysporum f. sp. lycopersici through bioagent. Indian Journal of Nematology, 30(1), 16-22.
• Bowers, J. H., Nameth, S. T., Riedel, R. M. and Rowe, R. C. (1996). Infection and colonization of potato roots by Verticillium dahliae as affected by Pratylenchus penetrans and P. crenatus. Phytopathology, 86(6), 614-621.
• Cayrol, J. C., C. Djian and L. Pijarowski (1989). Study of the nematicidal properties of the culture filtrate of the nematophagous fungus Paecilomyces lilacinus. Revue de Nematologie, 12(4): 331-336.
• Cayrol, J. C. and Djian, C. (1990). Study of the toxicity of Fusarium roseum var. arthrosporioides to the nematode Meloidogyne arenaria . Comptes-rendus de l’Academie d’Agriculture de France, 76(8), 121–129.
• Ciancio, A. (1995). Observations on the nematicidal properties of some mycotoxins. Fundamental and Applied Nematology, 18(5), 451-454.
• Dolar, F. S., Yeğin, N. Z. and Filiz, Ü. (2019). Kırşehir ve Kırıkkale illerinde buğday ve arpa ekim alanlarında görülen kök ve kök boğazı hastalıklarının belirlenmesi. Bitki Koruma Bülteni, 59(1), 71-84. Doi: https: //doi.org/10.16955/bitkorb.424930
• Dubois, T., and Coyne, D. L. (2011). Integrated pest management of banana. Banana breeding: progress and challenges. CRC Press, Washington DC.
• El-Borai, F. E., Duncan, L. W. and Graham, J. H. (2002a). Eggs of Tylenchulus semipenetrans inhibit growth of Phytophthora nicotianae and Fusarium solani in vitro. Journal of Nematology, 34(3), 267.
• El-Borai, F. E., Duncan, L. W. and Graham, J. H. (2002b). Infection of citrus roots by Tylenchulus semipenetrans reduces root infection by Phytophthora nicotianae. Journal of Nematology, 34(4), 384.
• Eriksen, G. S. (1998). Fusarium toxins in cereals: a risk assessment. Nordic Council of Ministers.
• Göze Ozdemir, F. G., Arici Ş. E., Yaşar, B and Elekçioglu İ. H. (2018). Effect Of Fusarium Culmorum Spore Suspension On Mortality Of Root Lesion Nematodes In Vitro Conditions. Black Sea Journal of Agriculture, 1, 51-54.
• Hallmann, J. and Sikora, R. A. (1996). Toxicity of fungal endophyte secondary metabolites to plant parasitic nematodes and soil-borne plant pathogenic fungi. European Journal of Plant Pathology, 102(2), 155-162.
• Hoseini, S. M. N., Pourjam, E. and Goltapeh, E. M. (2010). Synergistic studies on interaction of nematode-fungal system of tea plant in Iran. Journal of Agricultural Technology, 6(3), 487-496.
• Jones, M. G. K. and Fosu-Nyarko, J. (2014). Molecular biology of root lesion nematodes (Pratylenchus spp.) and their interaction with host plants. Annals of Applied Biology, 164 (2): 163-181.
• LaMondia, J. A. (2003). Interaction of Pratylenchus penetrans and Rhizoctonia fragariae in strawberry black root rot. Journal of Nematology, 35(1), 17.
• Mallaiah, B., Muthamilan, M., Prabhu, S. and Ananthan, R. (2014). Studies on interaction of nematode, Pratylenchus delattrei and fungal pathogen, Fusarium incarnatum associated with crossandra wilt in Tamil Nadu, India. Current Biotica, 8(2), 157-164.
• Mauza, B. E. & Webster, J. M. (1992). Suppression of alfalfa growth by concominitant population of Pratylenchus penetrans and two Fusarium sp. Journal of Nematology, 14(3): 364-367.
• Mani, A. and Sethi, C. L. (1986). Isolation and identification of nematoxins produced by Fusarium solani (mart) sacc. Indian Journal of Nematology, 16(2), 247-251.
• Miedaner, T., Cumagun, C. J. R. and Chakraborty, S. (2008). Population genetics of three important head blight pathogens Fusarium graminearum, F. pseudograminearum and F. culmorum. Journal of Phytopathology, 156(3), 129-139. Doi: https: //doi.org/10.1111/j.1439-0434.2007.01394.x
• Mwaura, P., Dubois, T., Losenge, T., Coyne, D. and Kahangi, E. (2010). Effect of endophytic Fusarium oxysporum on paralysis and mortality of Pratylenchus goodeyi. African Journal of Biotechnology, 9(8), 1130-1134.
• Nitao, J. K., Meyer, S. L. and Chitwood, D. J. (1999). In-vitro assays of Meloidogyne incognita and Heterodera glycines for detection of nematode-antagonistic fungal compounds. Journal of Nematology, 31(2), 172-183.
• Nitao, J. K., Meyer, S. L., Schmidt, W. F., Fettinger, J. C. and Chitwood, D. J. (2001). Nematode-antagonistic trichothecenes from Fusarium equiseti. Journal of Chemical Ecology, 27(5), 859-869.
• Poornima, K., Angappan, K., Kannan, R., Kumar, N., Kavino, M. and Balamohan, T. N. (2007). Interactions of nematodes with the fungal Panama wilt disease of banana and its management. Nematologia Mediterranea, 35(1), 35-39.
• Poole, G. J., Smiley, R. W., Paulitz, T. C., Walker, C. A., Carter, A. H., See, D. R. and Garland-Campbell, K. (2012). Identification of quantitative trait loci (QTL) for resistance to Fusarium crown rot (Fusarium pseudograminearum) in multiple assay environments in the Pacific Northwestern US. Theoretical and Applied Genetics, 125(1), 91-107. Doi: https: //doi.org/10.1007/s00122-012-1818-6
• Rotter, B. A. (1996). Invited review: Toxicology of deoxynivalenol (vomitoxin). Journal of Toxicology and Environmental Health Part A, 48(1), 1-34.
• Sankaralingam, A. and McGawley, E. C. (1994). Interrelationships of Rotylenchulus reniformis with Rhizoctonia solani on cotton. Journal of nematology, 26(4), 475.
• Scherm, B., Balmas, V., Spanu, F., Pani, G., Delogu, G., Pasquali, M., and Migheli, Q. (2013). Fusarium culmorum: causal agent of foot and root rot and head blight on wheat. Molecular plant pathology, 14(4), 323-341.
• Sikora, R. A., Niere, B. and Kimenju, J. (2003) Endophytic microbial biodiversity and plant nematode management in African agriculture. Biological control in IPM systems in Africa, 2, 179-192.
• Smiley, R. W. and Nicol, J. M. (2009). Nematodes which challange global wheat production. In Wheat: Science and trade. (pp. 171-187). Doi: https: //doi.org/10.1002/9780813818832.ch8
• Söğüt, M. A. and Devran, Z. (2011). Distribution and Molecular Identification of Root Lesion Nematodes in Temperate Fruit Orchards of Turkey. Nematropica, 41(1), 91-99. 
• Taheri, A., Hollamby, G. J., Vanstone, V. A. and Neate, S. M. (1994). Interaction between root lesion nematode, Pratylenchus neglectus (Rensch 1924) Chitwood and Oteifa 1952, and root rotting fungi of wheat. New Zealand Journal of Crop and Horticultural Science, 22(2), 181-185. Doi: https: //doi.org/10.1080/01140671.1994.9513823
• Turkington, T.K., Petran, A., Yonow, T. and Kriticos, D.J. (2014). Fusarium graminearum online. Retrieved from https: //www.researchgate.net/publication/313144182_Fusarium_graminearum_Pest_Geography
• Van Dessel, P., Coyne, D., Dubois, T., De Waele, D. and Franco, J. (2011). In vitro nematicidal effect of endophytic Fusarium oxysporum against Radopholus similis, Pratylenchus goodeyi and Helicotylenchus multicinctus. Nematropica, 41(2), 154-160.
• Vu, T., Sikora, R. and Hauschild, R. (2006). Fusarium oxysporum endophytes induced systemic resistance against Radopholus similis on banana. Journal of Nematology, 8(6), 847-852. Doi: https: //doi.org/10.1163/156854106779799259
• Yan, X. N., Sikora, R. A. and Zheng, J. W. (2011). Potential use of cucumber (Cucumis sativus L.) endophytic fungi as seed treatment agents against root-knot nematode Meloidogyne incognita. Journal of Zhejiang University Scıence B, 12(3), 219-225. Doi: https: //doi.org/10.1631/jzus.B1.000165
• Yavuzaslanoglu, E., I. H. Elekçioglu, J. M. Nicol, O. Yorgancilar and D. Hodson, 2012. Distribution, frequency and occurrence of cereal nematodes on the Central Anatolian Plateau in Turkey and their relationship with soil physicochemical properties. Nematology, 14 (7): 839-854. Doi: https: //doi.org/10.1163/156854112X631926
• Yavuzaslanoglu, E., M. S. Karaca, Ö. A. Sönmezoğlu, A. Öcal, I. H. Elekcioğlu and M. Aydoğdu, 2020. Occurrence and abundance of cereal nematodes in Konya and Karaman Provinces in Turkey. Turkish Journal of Entomology, 44 (2): 223-236. Doi: https://doi.org/10.16970/entoted.668561
• Yeates, G. W., T. Bongers, R. G. M. de Ggoede, D. W. Freckman and Georgieva, S.S. (1993). Feeding habits in soil nematode families and genera – an outline for soil ecologists. Journal of Nematology, 25 (3): 315-331.
• Yumurtaci, A., Sipahi, H., Al-Abdallat, A., Jighly, A. and Baum, M. (2017). Construction of new EST-SSRs for Fusarium resistant wheat breeding. Computational biology and chemistry, 68, 22-28. Doi: https: //doi.org/10.1016/j.compbiolchem.2017.02.003
• Zareen, A., Siddiqui, I. A., Aleem, F., Zaki, M. J. and Shaukat, S. S. (2001). Observations on the nematicidal effect of Fusarium solani on the root-knot nematode, Meloidogyne javanica. Journal of Plant Pathology, 207-214.
• Zuckerman, B. M., Mai, W. F. and Harrison, M. B. (1985). Plant Nematology Laboratory Manual. The University of Massachusetts Agricultural Experiment Station Amherst, Massachusetts 01003, pp. 212.