Screening for in vitro antioxidant activity and antifungal effect of Artemisia campestris L.

Nouı Hendel; Sarrı Djamel; Sarrı Madani; Mounir Selloum; Faiza Boussakra; Ouahiba Drıche

doi:10.31015/jaefs.2021.3.1

EN

Screening for in vitro antioxidant activity and antifungal effect of Artemisia campestris L.

Abstract

In this study, the methanolic extract (ME) and the essential oil (EO) of the medicinal plant Artemisia campestris L. were investigated for their antioxidant activity and their antifungal efficacy on the postharvest storage decays; Botrytis cinerea Pers. and Penicillium expansum Link. The total polyphenolic and flavonoid contents were determined. The ME had higher total polyphenolic and flavonoid contents (400.64 µg GAE/mg and 43.13 ± 0.14 μg QE/mg, respectively) than EO (27.47 ± 0.44 µg GAE/mg and 14.04 ± 0.82 µg QE/mg, respectively). The ME presented higher radical scavenging power than the BHT and its IC50 values were 11.71, 40.96 and 23.32 µg/mL for the DPPH, β-carotene bleaching and reducing power respectively. In the antifungal activity, the EO had the stronger effect on both molds, particularly at concentrations > 15 µL, ≥ 800 µL/L and ≥ 15 µL by fumigation, incorporation and disc-diffusion methods respectively, resulting in higher than 80% inhibition of B. cinerea mycelial growth, and from 50 to > 80% inhibition on P. expansum mycelial growth. Methanolic extract showed nearby 50% inhibition on both fungi. The EO MIC was less than 2.5 µL/mL which was shown as MFC for both molds. The bio-autography test has shown separated compounds of the ME having an inhibitor effect on spore germination. These results offer an advantage of suggesting A. campestris could be used as a material for extraction of certain antifungal chemicals for preventing spoilage in food items.

Keywords

Antifungal activity, Antioxidant activity, Artemisia campestris, Botrytis cinerea, Penicillium expansum

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Details

Primary Language

English

Subjects

Botany, Food Engineering, Horticultural Production

Journal Section

Research Article

Authors

Nouı Hendel ^*
0000-0002-6577-925X
Algeria

Sarrı Djamel
0000-0003-3617-1162
Algeria

Sarrı Madani
0000-0003-3617-1162
Algeria

Mounir Selloum This is me
0000-0002-0521-6842
Algeria

Faiza Boussakra This is me
0000-0002-1961-0429
Algeria

Ouahiba Drıche This is me
0000-0001-7904-7546
Algeria

Publication Date

September 15, 2021

Submission Date

February 12, 2021

Acceptance Date

May 29, 2021

Published in Issue

Year 2021 Volume: 5 Number: 3

DOI

https://doi.org/10.31015/jaefs.2021.3.1

IZ

https://izlik.org/JA67RN95RC

APA

Hendel, N., Djamel, S., Madani, S., Selloum, M., Boussakra, F., & Drıche, O. (2021). Screening for in vitro antioxidant activity and antifungal effect of Artemisia campestris L. International Journal of Agriculture Environment and Food Sciences, 5(3), 251-259. https://doi.org/10.31015/jaefs.2021.3.1

AMA

1.Hendel N, Djamel S, Madani S, Selloum M, Boussakra F, Drıche O. Screening for in vitro antioxidant activity and antifungal effect of Artemisia campestris L. int. j. agric. environ. food sci. 2021;5(3):251-259. doi:10.31015/jaefs.2021.3.1

Chicago

Hendel, Nouı, Sarrı Djamel, Sarrı Madani, Mounir Selloum, Faiza Boussakra, and Ouahiba Drıche. 2021. “Screening for in Vitro Antioxidant Activity and Antifungal Effect of Artemisia Campestris L”. International Journal of Agriculture Environment and Food Sciences 5 (3): 251-59. https://doi.org/10.31015/jaefs.2021.3.1.

EndNote

Hendel N, Djamel S, Madani S, Selloum M, Boussakra F, Drıche O (September 1, 2021) Screening for in vitro antioxidant activity and antifungal effect of Artemisia campestris L. International Journal of Agriculture Environment and Food Sciences 5 3 251–259.

IEEE

[1]N. Hendel, S. Djamel, S. Madani, M. Selloum, F. Boussakra, and O. Drıche, “Screening for in vitro antioxidant activity and antifungal effect of Artemisia campestris L”., int. j. agric. environ. food sci., vol. 5, no. 3, pp. 251–259, Sept. 2021, doi: 10.31015/jaefs.2021.3.1.

ISNAD

Hendel, Nouı - Djamel, Sarrı - Madani, Sarrı - Selloum, Mounir - Boussakra, Faiza - Drıche, Ouahiba. “Screening for in Vitro Antioxidant Activity and Antifungal Effect of Artemisia Campestris L”. International Journal of Agriculture Environment and Food Sciences 5/3 (September 1, 2021): 251-259. https://doi.org/10.31015/jaefs.2021.3.1.

JAMA

1.Hendel N, Djamel S, Madani S, Selloum M, Boussakra F, Drıche O. Screening for in vitro antioxidant activity and antifungal effect of Artemisia campestris L. int. j. agric. environ. food sci. 2021;5:251–259.

MLA

Hendel, Nouı, et al. “Screening for in Vitro Antioxidant Activity and Antifungal Effect of Artemisia Campestris L”. International Journal of Agriculture Environment and Food Sciences, vol. 5, no. 3, Sept. 2021, pp. 251-9, doi:10.31015/jaefs.2021.3.1.

Vancouver

1.Nouı Hendel, Sarrı Djamel, Sarrı Madani, Mounir Selloum, Faiza Boussakra, Ouahiba Drıche. Screening for in vitro antioxidant activity and antifungal effect of Artemisia campestris L. int. j. agric. environ. food sci. 2021 Sep. 1;5(3):251-9. doi:10.31015/jaefs.2021.3.1

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Screening for in vitro antioxidant activity and antifungal effect of Artemisia campestris L.

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