Statistical determination of in-vitro antimicrobial effects of extracts of marjoram (Origanum majorana L.) from Muğla, Turkey

Nur Ceyhan Güvensen; Dilek Keskin

doi:10.31015/jaefs.2021.3.19

EN

Statistical determination of in-vitro antimicrobial effects of extracts of marjoram (Origanum majorana L.) from Muğla, Turkey

Abstract

To determine the chemical composition and antimicrobial potential of marjoram (Origanum majorana L.) leaves extratcs obtained from Muğla, Turkey. The extract of ethanol, methanol, hexane, chloroform, isopropanol and water of marjoram were tested for antimicrobial activity against eleven bacterial strains and one yeast by disc diffusion method. The minimum inhibitory concentrations (MICs) of isopropanolic extract of marjoram leaves were determined using broth serial dilution method. The constitituents were analyzed by gas chromatography-mass spectrometry (GC/MS). The Shapiro-Wilk of Normal Distribution Test, Kruskal-Wallis and Mann-Whitney Tests were applied for statistical analysis in the study. All of the tested extracts, isopropanolic extracts of marjoram leaves showed the best inhibition zones against Klebsiella pneumoniae CCM 2318 (24 mm) and Candida albicans ATCC 10239 (28 mm), methanolic extracts against Staphylococcus aureus ATCC 6538/P (26 mm). In our study, the results of the inhibition zones are varied 6 to 26 mm against tested bacteria. The results of Mann-Whitney Tests showed that isopropanolic extract is a significant different from chloroform, water and hexane extracts, statistically (Pisopropanolic-chloroform=0.003, Pisopropanolic-water=0.000, Pisopropanolic-hexane=0.000). Isopropanolic extracts of O.marjorana were among the most active with the MIC values ranging from 0.008-64 mg/mL. MICs of O.marjorana L. different extracts obtained by the broth serial dilution method. The GC/MS analyses allowed fifteen compounds to be determined; the main constituents of the of isopropanolic extract of marjoram leaves was carvacrol %86.33. Carvacrol, major constituent of the marjoram isopropanolic leaf extract in our study, had possible antimicrobial activity by testing commercial carvacrol. It has been observed that the extracts can be used in the food industry to inhibit the growth of pathogens or as natural preservatives in foods.

Keywords

Origanum majorana L. (marjoram), Leaf extract, Antimicrobial activity, GC/MS analysis, Statistical analysis

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Details

Primary Language

English

Subjects

Food Engineering

Journal Section

Research Article

Authors

Nur Ceyhan Güvensen ^*
0000-0001-8753-2664
Türkiye

Dilek Keskin
0000-0003-2479-0070
Türkiye

Publication Date

September 15, 2021

Submission Date

March 29, 2021

Acceptance Date

July 9, 2021

Published in Issue

Year 2021 Volume: 5 Number: 3

DOI

https://doi.org/10.31015/jaefs.2021.3.19

IZ

https://izlik.org/JA47ST52GN

APA

Ceyhan Güvensen, N., & Keskin, D. (2021). Statistical determination of in-vitro antimicrobial effects of extracts of marjoram (Origanum majorana L.) from Muğla, Turkey. International Journal of Agriculture Environment and Food Sciences, 5(3), 398-404. https://doi.org/10.31015/jaefs.2021.3.19

AMA

1.Ceyhan Güvensen N, Keskin D. Statistical determination of in-vitro antimicrobial effects of extracts of marjoram (Origanum majorana L.) from Muğla, Turkey. int. j. agric. environ. food sci. 2021;5(3):398-404. doi:10.31015/jaefs.2021.3.19

Chicago

Ceyhan Güvensen, Nur, and Dilek Keskin. 2021. “Statistical Determination of In-Vitro Antimicrobial Effects of Extracts of Marjoram (Origanum Majorana L.) from Muğla, Turkey”. International Journal of Agriculture Environment and Food Sciences 5 (3): 398-404. https://doi.org/10.31015/jaefs.2021.3.19.

EndNote

Ceyhan Güvensen N, Keskin D (September 1, 2021) Statistical determination of in-vitro antimicrobial effects of extracts of marjoram (Origanum majorana L.) from Muğla, Turkey. International Journal of Agriculture Environment and Food Sciences 5 3 398–404.

IEEE

[1]N. Ceyhan Güvensen and D. Keskin, “Statistical determination of in-vitro antimicrobial effects of extracts of marjoram (Origanum majorana L.) from Muğla, Turkey”, int. j. agric. environ. food sci., vol. 5, no. 3, pp. 398–404, Sept. 2021, doi: 10.31015/jaefs.2021.3.19.

ISNAD

Ceyhan Güvensen, Nur - Keskin, Dilek. “Statistical Determination of In-Vitro Antimicrobial Effects of Extracts of Marjoram (Origanum Majorana L.) from Muğla, Turkey”. International Journal of Agriculture Environment and Food Sciences 5/3 (September 1, 2021): 398-404. https://doi.org/10.31015/jaefs.2021.3.19.

JAMA

1.Ceyhan Güvensen N, Keskin D. Statistical determination of in-vitro antimicrobial effects of extracts of marjoram (Origanum majorana L.) from Muğla, Turkey. int. j. agric. environ. food sci. 2021;5:398–404.

MLA

Ceyhan Güvensen, Nur, and Dilek Keskin. “Statistical Determination of In-Vitro Antimicrobial Effects of Extracts of Marjoram (Origanum Majorana L.) from Muğla, Turkey”. International Journal of Agriculture Environment and Food Sciences, vol. 5, no. 3, Sept. 2021, pp. 398-04, doi:10.31015/jaefs.2021.3.19.

Vancouver

1.Nur Ceyhan Güvensen, Dilek Keskin. Statistical determination of in-vitro antimicrobial effects of extracts of marjoram (Origanum majorana L.) from Muğla, Turkey. int. j. agric. environ. food sci. 2021 Sep. 1;5(3):398-404. doi:10.31015/jaefs.2021.3.19

Cited By

Origanum majorana L. Essential Oil-Coated Paper Acts as an Antimicrobial and Antioxidant Agent against Meat Spoilage

ACS Omega

https://doi.org/10.1021/acsomega.2c00237

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Abstract

Statistical determination of in-vitro antimicrobial effects of extracts of marjoram (Origanum majorana L.) from Muğla, Turkey

Abstract

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Origanum majorana L. Essential Oil-Coated Paper Acts as an Antimicrobial and Antioxidant Agent against Meat Spoilage

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