Investigation of antimicrobial properties and chemical composition of different extracts of Sweet gum leaves (Liquidambar orientalis)
Abstract
Different extracts of Liquidambar orientalisleaves were tested for their antimicrobial activity against eleven bacterial and one yeast strain by disc diffusion method. The antimicrobial activity was measured by disc diffusion and MIC. After that among extracts the best antimicrobial activity was detected.Volatile components of the ethanolic extracts of leaves of sweetgum analysed by GC/MS.
Ethanolic extracts of sweetgum leaves showed the best inhibition zones against Klebsiella pneumoniae (32mm). K.pneumoniae CCM 2318 showed the lowest sensitivity to 0.008 mg/mL concentration of ethanolic extracts. Ethanolic extracts of L. orientalis var. orientalis were showed the best antimicrobial activity MIC values of ethanolic extracts ranging from 0.008-64 mg.mL-1 to the tested bacteria. The GC/MS analyses allowed seven compounds to be determined; benzenepropanol (%49.30) and cinnamic acid (%35.89) were the main constituents of the of sweetgum leaves extract. All the extracts of L. orientalis leaves showed varying degrees of antimicrobial activity on the microorganisms tested. The antimicrobial activity was due to the presence the essential oils. Among the tested extracts the ethanolic extracts were the most active against the microorganisms tested compared to the reference antibiotics.
Keywords
antimicrobial activity, extract, Sweet gum, Chemical Composition, Leaves
Project Number
CMYO! 13-01
References
- Adams, R.P. (1995). Identification of essential oil components by gas chromatography/masss spectrometry. Allured Publishing:CarolStream,ILRetrivedfromhttps://www.scirp.org/(S(i43dyn45teexjx455qlt3d2q))/reference/ReferencesPapers.aspx?ReferenceID=394487
- Ali, N.A., W.D. Juelich, C. Kusnick and U. Lindequist (2001). Screening of Yemeni medicinal plants for antibacterial and cytotoxic activities. Journal of. Ethnopharmacology., 74, 173-179. Doi: 10.1016/s0378-8741(00)00364-0
- Basim, E. and H. Basim (2013). Antibacterial activity of Turkish endemic sweet gum (Liquidambar orientalis Mill. var. orientalis) storax against agricultural plant pathogenic bacteria and its use as a seed protectant. Journal of Food, Agriculture and Environment, Vol. 11, 2447-2450. . Retrived from[Google Scholar]
- Beatty, G.E., J. Provan (2010). Refugial persistence and postglacial recolonization of North America by the cold tolerant herbaceous plant Orthilia secunda. Molecular Ecology, 19, 5009-502. Retrived from [Google Scholar]
- Bradshaw, L.J. (1992). Laboratory microbiology. 4th Edn., Saunders College Publishing. Fort worth
- Fernandez, X., L. Lizzani-Cuvelier., A.M. Loiseau., C. Perichet., C. Delbecque., J.F. Arnaudo: (2005). Chemical composition of the essential oils from Turkish and Honduras styrax. Flavour and Fragrance Journal., 20, 70–73. Doi:10.1002/ffj.1370
- Gottlieb, O.R., Borin, M.R., de Brito, NR: (2002). Integration of ethnobotany and phytochemistry. dream or reality? Phytochemistry, 60,145-152. Doi: 10.1016/s0031-9422(02)00088-2.
- Ha¨nsel, R., Haas, H. (1984). Therapie mit Phytopharmaka; Springer: Berlin, Germany Retrived from[Google Scholar]
- Jananie, R.K., V. Priya., K, Vijayalakshmia: (2011). Determination of bioactive components of Cynodon dactylon by GC-MS Analysis. New York Science Journal, 4(4), 16-20. Retrived from[Google Scholar]
- Keskin, D., S. Toroglu: (2011). Studies on antimicrobial activities of solvent extracts of different commercial spices. Journal of .Environmental. Biology, 32, 251-256. Retrived from https://pubmed.ncbi.nlm.nih.gov/21882663/
