Breaking seed dormancy and regeneration in Cannabis sativa L.

Burak Önol; Mehmet Uğur Yıldırım

doi:10.31015/jaefs.2021.4.32

EN

Breaking seed dormancy and regeneration in Cannabis sativa L.

Abstract

Cannabis sativa L. is an important medicinal plant species that grow under natural conditions and has been legalized in 20 out of 81 Turkish provinces. The female inflorescence is a highly branched compound raceme with indeterminate habit of growth. This results in different maturing of seeds on the inflorescence and induce physiological dormancy on seeds. The study aimed to improve seed germination percentage using various concentrations of GA₃, GA₃ + BAP, germination on water and water solidified with agar, MS or Gamborg B5 medium. The results showed that the best seed germination was noted on Gamborg B5 medium. Different explants were used to regenerated plantlets on Gamborg B5 medium. All explants were suitable for callus regeneration variably. Only the stem nodes of Samsun Vezirköprü were suitable to induce shoots and plantlets. These plantlets were acclimatized on clay loam soils and transferred to field condition during October 2020, where they acclimatized successfully. These studies provide an effective insight into the mechanism seed dormancy in C. sativa. Further studies using other plant growth regulator concentrations will improve shoot regeneration and aid in utilizing the methods for breeding purpose.

Keywords

Hemp, In vitro, mass propagation, seed germination, TDZ

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Details

Primary Language

English

Subjects

Agricultural Engineering (Other)

Journal Section

Research Article

Authors

Burak Önol ^*
0000-0003-3114-558X
Türkiye

Mehmet Uğur Yıldırım
0000-0002-7419-0682
Türkiye

Publication Date

December 15, 2021

Submission Date

August 22, 2021

Acceptance Date

October 1, 2021

Published in Issue

Year 2021 Volume: 5 Number: 4

DOI

https://doi.org/10.31015/jaefs.2021.4.32

IZ

https://izlik.org/JA29JJ57XW

APA

Önol, B., & Yıldırım, M. U. (2021). Breaking seed dormancy and regeneration in Cannabis sativa L. International Journal of Agriculture Environment and Food Sciences, 5(4), 709-719. https://doi.org/10.31015/jaefs.2021.4.32

AMA

1.Önol B, Yıldırım MU. Breaking seed dormancy and regeneration in Cannabis sativa L. int. j. agric. environ. food sci. 2021;5(4):709-719. doi:10.31015/jaefs.2021.4.32

Chicago

Önol, Burak, and Mehmet Uğur Yıldırım. 2021. “Breaking Seed Dormancy and Regeneration in Cannabis Sativa L”. International Journal of Agriculture Environment and Food Sciences 5 (4): 709-19. https://doi.org/10.31015/jaefs.2021.4.32.

EndNote

Önol B, Yıldırım MU (December 1, 2021) Breaking seed dormancy and regeneration in Cannabis sativa L. International Journal of Agriculture Environment and Food Sciences 5 4 709–719.

IEEE

[1]B. Önol and M. U. Yıldırım, “Breaking seed dormancy and regeneration in Cannabis sativa L”., int. j. agric. environ. food sci., vol. 5, no. 4, pp. 709–719, Dec. 2021, doi: 10.31015/jaefs.2021.4.32.

ISNAD

Önol, Burak - Yıldırım, Mehmet Uğur. “Breaking Seed Dormancy and Regeneration in Cannabis Sativa L”. International Journal of Agriculture Environment and Food Sciences 5/4 (December 1, 2021): 709-719. https://doi.org/10.31015/jaefs.2021.4.32.

JAMA

1.Önol B, Yıldırım MU. Breaking seed dormancy and regeneration in Cannabis sativa L. int. j. agric. environ. food sci. 2021;5:709–719.

MLA

Önol, Burak, and Mehmet Uğur Yıldırım. “Breaking Seed Dormancy and Regeneration in Cannabis Sativa L”. International Journal of Agriculture Environment and Food Sciences, vol. 5, no. 4, Dec. 2021, pp. 709-1, doi:10.31015/jaefs.2021.4.32.

Vancouver

1.Burak Önol, Mehmet Uğur Yıldırım. Breaking seed dormancy and regeneration in Cannabis sativa L. int. j. agric. environ. food sci. 2021 Dec. 1;5(4):709-1. doi:10.31015/jaefs.2021.4.32

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Abstract

Breaking seed dormancy and regeneration in Cannabis sativa L.

Abstract

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Details

Primary Language

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Acceptance Date

Published in Issue

DOI

IZ

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Cited By

Challenges and potentials of new breeding techniques in Cannabis sativa

Seed germination behavior of Teucrium santae Quézel & Simonneau: a vulnerable and endemic Lamiaceae (Northwest Algeria)

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