Isolation of PET-degrading Bacteria from an Old Garbage Dump and Investigation of Their PET-degrading Potential

Esma Ceylan; Aleyna Nalcaoglu Senocak; Dilsat Nigar Colak; Ali Osman Beldüz; Kadriye İnan; Nuran Kahriman; Sabriye Çanakçı

doi:10.35206/jan.1749818

EN TR

Isolation of PET-degrading Bacteria from an Old Garbage Dump and Investigation of Their PET-degrading Potential

Abstract

The increased use of plastic materials, including polyethylene terephthalate (PET), has caused serious environmental pollution. Due to the lack of effective methods for PET degradation and recycling, new methods such as microbial plastic degradation are being investigated. In this study, soil samples were collected from an old waste dumping site located in Trabzon/Turkiye and screened for PET-degrading bacteria. Three isolates were determined to use PET as sole carbon source. Based on 16S rRNA sequences, strain PETDeg 3 and 4 were identified as Bacillus licheniformis, and strain PETDeg 5 as Acinetobacter junii. Biodegradation of PET by bacteria was assessed using weight loss, Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM) methods. In a 28 days of degradation study using PET films, incubation with PETDeg 3, PETDeg 4, and PETDeg 5 resulted in 0.42%, 0.75%, and 0.25% of weight loss respectively. SEM revealed obvious structural changes such as roughness, fissures and cracks on the surfaces of the PET films.

Keywords

Acinetobacter junii , Bacillus licheniformis , Biodegradation , FTIR , PET , SEM

Polietilen Tereftalat (PET) Parçalayan Bakterilerin Eski Bir Çöp Depolama Alanından İzole Edilmesi ve PET Biyobozunum Potansiyellerinin İncelenmesi

Öz

Plastik materyallerin, özellikle polietilen tereftalat (PET) kullanımının artması, ciddi çevresel kirlilik problemlerine yol açmaktadır. PET’in doğada kalıcılığı ve mevcut geri dönüşüm yöntemlerinin yetersizliği, mikrobiyal bozunma gibi alternatif çözüm arayışlarını gündeme getirmiştir. Bu çalışmada, Trabzon/Türkiye’de bulunan eski bir atık döküm sahasından toprak örnekleri alınarak PET bozunumu gerçekleştirebilen bakteriler taranmıştır. Yapılan ön tarama sonucunda üç farklı bakteri izolatının PET’i karbon kaynağı olarak kullanabildiği belirlenmiştir. 16S rRNA gen dizilim analizlerine göre, PETDeg-3 ve PETDeg-4 suşları Bacillus licheniformis, PETDeg-5 suşu ise Acinetobacter junii olarak tanımlanmıştır. Bakteriyel PET biyobozunumu; ağırlık kaybı, Fourier Dönüşümlü Kızılötesi Spektroskopisi (FTIR) ve Taramalı Elektron Mikroskobu (SEM) analizleri ile değerlendirilmiştir. PET film kullanılarak gerçekleştirilen 28 günlük biyobozunma deneyinde, PETDeg-3, PETDeg-4 ve PETDeg-5 suşları ile inkübasyon sonucunda sırasıyla %0,42, %0,75 ve %0,25 oranında ağırlık kaybı tespit edilmiştir. SEM analizleri, PET film yüzeylerinde pürüzlülük, çatlaklar ve yarıklar gibi belirgin yapısal bozulmaların meydana geldiğini göstermiştir. Elde edilen sonuçlar, bu suşların PET biyobozunumu potansiyeline sahip olduğunu ve çevresel plastik kirliliğinin azaltılmasında biyoteknolojik uygulamalarda kullanılabileceklerini göstermektedir.

Anahtar Kelimeler

Acinetobacter junii , Bacillus licheniformis , Biyobozunum , FTIR , PET , SEM

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Kimya Mühendisliği (Diğer)

Bölüm

Araştırma Makalesi

Yazarlar

Esma Ceylan
0000-0002-5559-8869
Türkiye

Aleyna Nalcaoglu Senocak
0000-0003-2528-8744
Türkiye

Dilsat Nigar Colak
0000-0001-9544-3733
Türkiye

Ali Osman Beldüz
0000-0003-2240-7568
Türkiye

Kadriye İnan
0000-0002-5909-588X
Türkiye

Nuran Kahriman
0000-0001-9729-433X
Türkiye

Sabriye Çanakçı ^*
0000-0003-0132-7198
Türkiye

Yayımlanma Tarihi

31 Aralık 2025

Gönderilme Tarihi

30 Temmuz 2025

Kabul Tarihi

20 Ekim 2025

Yayımlandığı Sayı

Yıl 2025 Cilt: 8 Sayı: 2

DOI

https://doi.org/10.35206/jan.1749818

IZ

https://izlik.org/JA82FM39JJ

APA

Ceylan, E., Nalcaoglu Senocak, A., Colak, D. N., Beldüz, A. O., İnan, K., Kahriman, N., & Çanakçı, S. (2025). Isolation of PET-degrading Bacteria from an Old Garbage Dump and Investigation of Their PET-degrading Potential. Journal of Apitherapy and Nature, 8(2), 229-252. https://doi.org/10.35206/jan.1749818

AMA

1.Ceylan E, Nalcaoglu Senocak A, Colak DN, vd. Isolation of PET-degrading Bacteria from an Old Garbage Dump and Investigation of Their PET-degrading Potential. Journal of Apitherapy and Nature. 2025;8(2):229-252. doi:10.35206/jan.1749818

Chicago

Ceylan, Esma, Aleyna Nalcaoglu Senocak, Dilsat Nigar Colak, vd. 2025. “Isolation of PET-degrading Bacteria from an Old Garbage Dump and Investigation of Their PET-degrading Potential”. Journal of Apitherapy and Nature 8 (2): 229-52. https://doi.org/10.35206/jan.1749818.

EndNote

Ceylan E, Nalcaoglu Senocak A, Colak DN, Beldüz AO, İnan K, Kahriman N, Çanakçı S (01 Aralık 2025) Isolation of PET-degrading Bacteria from an Old Garbage Dump and Investigation of Their PET-degrading Potential. Journal of Apitherapy and Nature 8 2 229–252.

IEEE

[1]E. Ceylan vd., “Isolation of PET-degrading Bacteria from an Old Garbage Dump and Investigation of Their PET-degrading Potential”, Journal of Apitherapy and Nature, c. 8, sy 2, ss. 229–252, Ara. 2025, doi: 10.35206/jan.1749818.

ISNAD

Ceylan, Esma - Nalcaoglu Senocak, Aleyna - Colak, Dilsat Nigar - Beldüz, Ali Osman - İnan, Kadriye - Kahriman, Nuran - Çanakçı, Sabriye. “Isolation of PET-degrading Bacteria from an Old Garbage Dump and Investigation of Their PET-degrading Potential”. Journal of Apitherapy and Nature 8/2 (01 Aralık 2025): 229-252. https://doi.org/10.35206/jan.1749818.

JAMA

1.Ceylan E, Nalcaoglu Senocak A, Colak DN, Beldüz AO, İnan K, Kahriman N, Çanakçı S. Isolation of PET-degrading Bacteria from an Old Garbage Dump and Investigation of Their PET-degrading Potential. Journal of Apitherapy and Nature. 2025;8:229–252.

MLA

Ceylan, Esma, vd. “Isolation of PET-degrading Bacteria from an Old Garbage Dump and Investigation of Their PET-degrading Potential”. Journal of Apitherapy and Nature, c. 8, sy 2, Aralık 2025, ss. 229-52, doi:10.35206/jan.1749818.

Vancouver

1.Esma Ceylan, Aleyna Nalcaoglu Senocak, Dilsat Nigar Colak, Ali Osman Beldüz, Kadriye İnan, Nuran Kahriman, Sabriye Çanakçı. Isolation of PET-degrading Bacteria from an Old Garbage Dump and Investigation of Their PET-degrading Potential. Journal of Apitherapy and Nature. 01 Aralık 2025;8(2):229-52. doi:10.35206/jan.1749818

e-ISSN: 2667-4734 Yayın Modeli: Sürekli Yayın Atıf Dizinleri: TR Dizin

Isolation of PET-degrading Bacteria from an Old Garbage Dump and Investigation of Their PET-degrading Potential

Abstract

Keywords

Polietilen Tereftalat (PET) Parçalayan Bakterilerin Eski Bir Çöp Depolama Alanından İzole Edilmesi ve PET Biyobozunum Potansiyellerinin İncelenmesi

Öz

Anahtar Kelimeler

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

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