Preliminary Studies: The Potential Anti-Angiogenic Activities of Two Sulavesi Island (Indonesia) Propolis and Their Chemical Characterization
Öz
Several studies have
previously reported propolis, or its constituents, to inhibit tumour
angiogenesis1. The anti-angiogenic
activity of two Indonesian stingless bee propolis extracts from Sulawesi Island
on vascular cells were assessed. Sample D01 was obtained from the outer side of
bee hives, while D02 was from the inner side of the same hives. Preliminary
results of cell viability and multi-channel wound healing assays on HUVECs
and placenta-derived pericytes demonstrated that D01 elicited a strong cytotoxic
effect and a considerable anti-migratory activity on the vascular cells. Although D02 demonstrated a much weaker cytotoxic
effect on the cell lines compared to D01, it elicited a substantial protective effect on the pericytes against CoCl2-induced dropout in an
experiment to mimic a micro-environment commonly
associated with angiogenesis and tumour growth2. These results demonstrate
modulatory effects of these propolis samples in vascular cells, which requires further
investigation. The extracts were
profiled by using liquid chromatography coupled to high resolution mass
spectrometry. The
most abundant compounds in Indonesian sample D01 from LC-MS analysis seem to be unusual since they do not immediately
fall into a clear class such as flavonoids or terpenoids. Two of the most
abundant compounds
have elemental compositions matching actinopyrones which are antibiotic
compounds isolated from Streptomyces pactum3. Identification on the basis of elemental composition is not definitive
but compounds in D01
are possibly due to unusually modified
terpenoids. Sample D02
has abundant compounds which include four related diterpenes with differing
degrees of oxygenation and some sesquiterpenes.
However, again the profile is unusual.
Anahtar Kelimeler
Kaynakça
- 1. Daleprane JB and Abdalla DS (2013) Emerging roles of propolis: antioxidant, cardioprotective, and antiangiogenic actions. Evid Based Complement Alternat Med, 2013:175135.
- 2. Raza A, Franklin MJ, and Dudek AZ (2010) Pericytes and vessel maturation during tumor angiogenesis and metastasis. Am J Hematol 85(8):593-8.
- 3. Hayakawa Y, Saito J, Izawa M, and Shin-ya K (2014) Actinopyrone D, a new downregulator of the molecular chaperone GRP78 from Streptomyces sp. J Antibiotics 67: 831–834