In vitro Assessments of Cytotoxic and Cytostatic Effects of Propolis in Cells from the Human Colon Carcinoma Cell Line (HCT 116)
Öz
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Based on knowledge
from traditional Arab medicine, this in vitro study aims to
evaluate cytotoxic and cytostatic effects of traditionally used as
anti-cancer propolis from four different regions in Morocco. Propolis samples
were collected from different locations in Morocco, which are characterized
by different plant predominant vegetations. The resin, wax and balsam of
hydroalcoholic extract of propolis content were identified, and the
antioxidant content was analyzed with the use of HPLC and colorimetric
methods. The antioxidant activity was assessed by DPPH, ABTS and ferric
reducing power assays. Cells from the human colon carcinoma cell line (HCT 116)
were treated for 24h (cytotoxic effects) and 72h (cytostatic effects)
with increasing concentrations (0-1000 µg/ml) of methanol extracts from the
four propolis (N1, N2, NC, PJ). Results obtained using the MTT assay indicate that N1, NC
and PJ showed no significant difference between their cytotoxic and
cytostatic effects with IC50 of 300, 750 and 700 µg/ml, respectively. A clear and
highly significant cytostatic effects were
measured with N2-extract with IC50 of 750 µg/ml. Furthermore, propolis with high resin and low wax content had high level of
antioxidant compounds, and strong antioxidant and anticancer activities. These results indicate
that the traditionally known anti-cancer effects of N2-extract might be mediated in part through cytostatic effects. |
Anahtar Kelimeler
Kaynakça
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