Cytotoxic Activity of Four Propolis Colombian Samples Against Canine Osteosarcoma Cells
Öz
The cytotoxicity of
propolis against tumor cells has been widely reported1. Propolis
from tropical areas present components such as triterpenes, diterpenes and
benzophenones2, however, new types of propolis may be recognized and
their biological activities need to be elucidated. Therefore, four Colombian
propolis samples were chemically characterized by gas chromatography-mass
spectrometry (GC–MS) and their cytotoxic action against canine osteosarcoma
(OSA) cells was determined, as well as apoptosis induction and changes in gene
expression of CASPASE 8 and CASPASE 9. OSA cells were treated
with ethanolic extracts of propolis; the lactate dehydrogenase (LDH) assay and
the annexin V/propidium iodide kit were used to evaluate the cytotoxic effect
and apoptosis induction, respectively. Changes in gene expression were evaluated
by quantitative real time PCR (qPCR). The propolis samples were rich in
diterpenes, triterpenes and benzophenones. Propolis samples induced a cytotoxic
effect towards OSA cells that was correlated to the LDH leakage into the
culture medium after cell membrane rupture. The cytotoxic effect increased in a
time-concentration manner after incubation with propolis and the cytotoxic
concentration 50% (CC50) was lower than 36.24 µg/ml after 72 h of
treatment. Colombian propolis samples induced total apoptosis significantly
(untreated cells: 10.77 ± 2.22%; treated cells: from 58.403% ±1.39 to 95.97 ±
2.14% after 48 h). All propolis samples increased the expression of CASPASE
8 and three of them induced the expression of CASPASE 9. Colombian
propolis samples induced apoptosis in canine OSA cells, suggesting their
promising potential as news strategies for cancer therapy.
Acknowledgements: Universidad Antonio Nariño, Bogotá Colombia
Anahtar Kelimeler
Kaynakça
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- 2. Castro V, Negri G, Salatino A, Bandeir M, (2011). A new type of Brazilian propolis: prenylated benzophenones in propolis from Amazon and effects against cariogenic bacteria. Food Chemistry, 125, 966-972.