Caseinates Loaded with Red Propolis Extract
Öz
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This work aimed
to characterize microcapsules of caseinates loaded with red propolis extract
(RPE) using different analytical and biological methods. Five compositions of
microcapsules of caseinates loaded with RPE at concentrations between 10% and
50% were prepared using sodium casein from bovine milk as encapsulating agent
and colloidal silicon dioxide as a flow agent. The caseinates were produced
using colloidal dispersion technique and submitted to the spray-drying
technique. The caseinates were characterized using SEM analysis, thermal
analysis, FTIR, Fluorescence analysis, electrophoresis, dissolution studies,
antioxidant and antibacterial activities.
SEM images showed spherical particles in the range from 3.24 μm to
20.5 μm. FTIR, NMR spectra in
solid-state and thermal analysis also demonstrated the encapsulation of the
RPE. Dissolution study revealed limited to poor water-solubility of flavonoids
in the compositions CSRPE18% and CSRPE28%, but good water solubility of
naringenin in the compositions CSRPE35% and CSRPE50%. Excellent antioxidant
activity and antibacterial activity were demonstrated only from CSRPE50% and
CSRPE35% compositions. Thermal analysis, water-solubility dissolution assay
and antioxidant activity have indicated a high protein binding between sodium
casein and flavonoids from RPE in the CSRPE18% and CSRPE28% compositions
resulting in a controlled release of the flavonoid. However immediate release
of flavonoids in the compositions CSRPE35% and CSRPE50% was detected. The
Fluorescence and thermal analysis allied to the dissolution, antioxidant and
antibacterial tests were important analytical techniques to elucidate the
interaction between flavonoid-protein and flavonoid release kinetic from
caseinates soluble complex.
Acknowledgments. The authors are grateful to CNPq, CAPES, FINEP and FAPEAL for financial support.
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