Comparative Study of Commercial Propolis Based Products – Antibacterial Activity and Bioactive Compounds
Öz
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Propolis is one
of the best natural antibiotic, with high amounts of polyphenols, derived
from plant’s resins from where the bees collect, mix with own substances and
wax, and preserve it in the hive. High variability among propolis samples was
noticed, depending on the geographical origin, plant source, harvesting
procedures and methods of evaluation1. One of the major problems
in labeling the propolis that is commercially available is the real bioactive
compounds concentration. The study was aimed to evaluate the in vitro antibacterial activity and
the chemical composition of different commercially available propolis based
products (tinctures, ethanolic, glycolic and hydroglycolic extracts, products
for veterinary and human use). Spectrophotometric and chromatographic
determinations were used to characterize the propolis products. The in vitro antibacterial activity was
evaluated against reference strains of Staphylococcus
aureus, Staphylococcus pseudintermedius, Bacillus cereus, Enterococcus
faecalis, Salmonella enteritis, Salmonella typhimurium and Escherichia coli using the disc
diffusion assay. The results indicated intense antibacterial properties in
case of both tinctures and ethanolic extracts, especially against Bacillus cereus, Staphylococcus aureus and Staphylococcus
pseudintermedius. While the inhibition zone diameters were significantly
smaller for the Gram negative bacteria, no efficacy was recorded towards Enterococcus faecalis. Total
polyphenols varied from 18-26% and flavonoids (6-10%) were determined in all
samples. The total amount of polyphenols and flavonoids depended both on the
concentration of propolis in the extracts and the solvent type used for
extract preparations and its concentration. These data underline the complex
chemical composition and antimicrobial potential of certain propolis based
products. |
Anahtar Kelimeler
Kaynakça
- Bankova V (2005) Chemical diversity of propolis and the problem of standardization. Journal of Ethnopharmacology 100: 114-117