A Preliminary Study: The Selective Effect of Bee Venom on Inhibition of Cell Migration in Metastatic Breast Cancer Cells Over Normal Cells in Comparison with Cisplatin
Öz
Bee venom is considered as an effective alternative medication in the treatment of some diseases, such cancer, but it has not been applied to patients routinely. Bee venom has in vitro selective cytotoxic effect on some cancer cells examined but the mechanism of action has not been fully elucidated. Cancer is characterized by not only uncontrolled and increased cell proliferation but also by invasive and metastatic properties of proliferating cells. Wound healing assay is used for revealing the migration rate of cells in vitro. The effect of bee venom on healing diabetes-induced wound has been shown before, but to the best of knowledge there is no study showing migration velocity of metastatic breast cancer cells after bee venom treatment. This study aimed (i) to reveal the metastatic behaviour of metastatic breast cancer cells after bee venom compared to cisplatin, and (ii) also to conclude the differences between normal and cancer cells in terms of migration after bee venom and cisplatin. Findings showed that bee venom was more effective on selective inhibition of cancer cell migration, but cisplatin-mediated inhibitory effect on metastasis could not be detected as even low concentrations of cisplatin induced high rate of cell death. The preliminary findings need to be expanded to cover more cell lines and the mechanism for this effect should be disclosed by detailed molecular methodologies for the future as well.
Anahtar Kelimeler
Apitherapy, cancer, cell migration, wound healing
Teşekkür
Authors thank to Mr Sinan Tetikoglu (Department of Molecular Biology and Genetics, Karadeniz Technical University) for assisting experimental background, and to Prof Sevgi Kolayli (Department of Biochemistry, Karadeniz Technical University) for bee venom supply.
Kaynakça
- Badr, G., Hozzein, W. N., Badr, B. M., Al Ghamdi, A., Eldien, H. M. S., & Garraud, O. (2016). Bee Venom Accelerates Wound Healing in Diabetic Mice by Suppressing Activating Transcription Factor-3 (ATF-3) and Inducible Nitric Oxide Synthase (iNOS)-Mediated Oxidative Stress and Recruiting Bone Marrow-Derived Endothelial Progenitor Cells. Journal of Cellular Physiology, 231(10), 2159-2171. doi:10.1002/jcp.25328
- Duffy, C., Sorolla, A., Wang, E., Golden, E., Woodward, E., Davern, K., . . . Blancafort, P. (2020). Honeybee venom and melittin suppress growth factor receptor activation in HER2-enriched and triple-negative breast cancer. Npj Precision Oncology, 4(1). doi:ARTN 24 10.1038/s41698-020-00129-0
- Flake, G., Moore, B., Sutton, D., Kissling, G., Horton, J., Wicker, B., . . . Dixon, D. (2013). The natural history of uterine leiomyomas: light and electron microscopic studies of fibroid phases, interstitial ischemia, inanosis, and reclamation. Obstet Gynecol Int, 528376.
- Gandalovicova, A., Rosel, D., Fernandes, M., Vesely, P., Heneberg, P., Cermak, V., . . . Brabek, J. (2017). Migrastatics-Anti-metastatic and Anti-invasion Drugs: Promises and Challenges. Trends in Cancer, 3(6), 391-406. doi:10.1016/j.trecan.2017.04.008
- Grada, A., Otero-Vinas, M., Prieto-Castrillo, F., Obagi, Z., & Falanga, V. (2017). Research Techniques Made Simple: Analysis of Collective Cell Migration Using the Wound Healing Assay. Journal of Investigative Dermatology, 137(2), E11-E16. doi:10.1016/j.jid.2016.11.020
- Han, S., Lee, K., Yeo, J., Kim, W., & Park, K. (2011). Biological effects of treatment of an animal skin wound with honeybee (Apis melifera. L) venom. Journal of Plastic Reconstructive and Aesthetic Surgery, 64(3), E67-E72. doi:10.1016/j.bjps.2010.08.022
- Horak, C. E., Lee, J. H., Marshall, J. C., Shreeve, S. M., & Steeg, P. S. (2008). The role of metastasis, suppressor genes in metastatic dormancy. Apmis, 116(7-8), 586-601. doi:DOI 10.1111/j.1600-0463.2008.01027.x
- Hozzeinab, W. N., Badr, G., Badr, B. M., Allam, A., Al Ghamdi, A., Al-Wadaan, M. A., & Al-Waili, N. S. (2018). Bee venom improves diabetic wound healing by protecting functional macrophages from apoptosis and enhancing Nrf2, Ang-1 and Tie-2 signaling. Molecular Immunology, 103, 322-335. doi:10.1016/j.molimm.2018.10.016
- Maeng, Y., Kim, K. T., Zhou, X., Jin, L. T., Kim, K. S., Kim, Y. H., . . . Li, X. K. (2018). A novel microbial technique for producing high-quality sophorolipids from horse oil suitable for cosmetic applications. Microbial Biotechnology, 11(5), 917-929. doi:10.1111/1751-7915.13297
- Poulose, N., Sajayan, A., Ravindran, A., Sreechithra, T. V., Vardhan, V., Selvin, J., & Kiran, G. S. (2020). Photoprotective effect of nanomelanin-seaweed concentrate in formulated cosmetic cream: With improved antioxidant and wound healing properties. Journal of Photochemistry and Photobiology B-Biology, 205. doi:10.1016/j.jphotobiol.2020.111816